秋水仙素诱导青虾次级卵母细胞二倍体初探

以青虾卵巢为材料，采用秋水仙素诱导多倍体次级卵母细胞．经滴片试验，确立此实验最佳滴片高度为1．5cm，通过设置5个秋水仙素浓度(0．08～0．40mg／mL)及3个作用时间(6～24h)，观察发生染色体加倍的细胞相对数目．实验结果表明：在使用秋水仙素的浓度为0．08～0．40mg／mL，处理6～24h时都可以获得多倍体卵细胞．在秋水仙素浓度是0．32mg／mL，处理6h可得到最高二倍体突变率(32．58％)．还测试了0．32mg／mL COM作用6h时ATPase活性与生理盐水对照组的变化，结果显示COM会在一定程度上影响细胞的新陈代谢．但是50％左右的成活率表明，这种诱导突变的方法仍具有一定的应用价值．     

适应市场需求积极推进吕梁农业结构高速调整

文章结合吕梁地区实际，对如何适应市场需求确立农村经济结构调整方向和进行产业化经营进行了论证分析。     

谈旧桥加固和桥梁病害处理技术

根据山西省现有公路和桥梁状况，为恢复和提高旧桥的承载能力及通过能力，延长桥梁使用寿命，迫切需要进行评价和加固改造。     

大学《现代教育技术》课程教学中探究式学习模式的应用与思考

本文从探究式学习的定义和特征出发,反对不分教学对象差异的"唯探究主义",在大学《现代教育技术》课程的教学中倡导面向全体学生,开展多样化的探究式学习。这就要求在教学过程中,辩证地处理学生自主与教师指导的关系,强调教师与学生的相互倾听,珍视探究中学生的个人观念、独特感受和体验,并引导学生积极反思。     

模糊Riemann-Stieltjes积分的收敛定理

模糊Riemann-Stieltjes积分是模糊分析中的一类重要的模糊积分,但相应的积分序列的收敛定理尚未见到.将给出模糊数值函数列关于实函数和模糊数值函数关于实函数列的两类模糊Riemann-Stiehjes积分序列的收敛定理.     

Un curare d'une Erythrine africaine

Summary An alcaloid C₁₆H₁₉NO₃ has been isolated fromErythrina tholloniana; the iodohydrate of this erythroidine has a melting point of 225°C and 239°C for its chlorhydrate. It has a powerful curare-like action on the frog or on its isolated sciatic-sartorius preparation; at a concentration of less than 1/1,000,000, a complete neuromuscular block is produced: the electrical stimulation of the motor nerve does not produce any contraction, but the muscle reacts by an end-plate potential having the same characteristics (shape, duration, possibility of summation) as the electrical waves produced in the same preparation curarized by ordinary curare or by quaternary ammonium derivatives. Decurarization by veratrine 1/200,000 is accompanied by the same electrical reactions as those which have been described in preparations treated by curare.On mammals, the alcaloid has little curariform activity; on the isolated phrenic-diaphragm preparation of the rat, incomplete block was produced at a concentration of 1/5000.     

ADO意义.NET在并发控制方面存在的问题及解决方案

针对ADO.NET在处理"丢失的修改"问题时,不支持"关键字和已修改字段"的并发控制问题提出了相应的解决办法;同时对ADO.NET架构本身带来的异常更新问题提供了解决思路.     

Structural Changes of Water Molecules Upon the Reduction of Quinones in The Reaction Center from Rhodobactery Sphaeroides

1 Results The photosynthetic bacterial reaction center (RC) is a membrane protein complex.The RC is composed of three protein subunits and redox components such as bacteriochlorophylls, bacteriopheophytins,and quinones.The RC performs the photochemical electron transfer from the bacteriochlorophyll dimer through a series of electron donor and acceptor molecules to a secondary quinone,QB.QB accepts electrons from a primary quinone,QA,in two sequential electron transfer reactions.The second electron trans...