Effects of insulin hypoglycaemia on the adrenal medulla of the hamster

Résumé Pendant l'hypoglycémie insulinique (4 U/100 g), le hamster maintenu a 19°C présente une augmentation de l'adrénaline et de la noradrénaline plasmatiques, ainsi qu'une réduction de ces deux catécholamines dans les surrénales. Maintenu à 26°C, l'hypoglycémie se produit aussi bien avec 4 U/100 g d'insuline mais il n'y a pas de changements de l'adrénaline et noradrénaline du plasma et des surrénales. Pour obtenir des modifications, il est nécessaire d'augmenter la dose d'insuline à 8 U/100 g. En conclusion, la température extérieure a une influence marquée sur la réponse médullo-surrénale de l'hypoglycémie insulinique.     

Hypophysial feed-back in the hypothalamic regulation of luteinizing hormone secretion

Resumen LH exógeno disminuye LHRF circulante no así estradiol ni estradiol-progesterona, concluyéndose que: eminencia media regula LH siendo su nivel plasmático la señal feed-back.     

Structural and functional conservation of key domains in InsP3 and ryanodine receptors

Inositol-1,4,5-trisphosphate receptors (InsP(3)Rs) and ryanodine receptors (RyRs) are tetrameric intracellular Ca(2+) channels. In each of these receptor families, the pore, which is formed by carboxy-terminal transmembrane domains, is regulated by signals that are detected by large cytosolic structures. InsP(3)R gating is initiated by InsP(3) binding to the InsP(3)-binding core (IBC, residues 224-604 of InsP(3)R1) and it requires the suppressor domain (SD, residues 1-223 of InsP(3)R1). Here we present structures of the amino-terminal region (NT, residues 1-604) of rat InsP(3)R1 with (3.6??) and without (3.0??) InsP(3) bound. The arrangement of the three NT domains, SD, IBC-β and IBC-α, identifies two discrete interfaces (α and β) between the IBC and SD. Similar interfaces occur between equivalent domains (A, B and C) in RyR1 (ref. 9). The orientations of the three domains when docked into a tetrameric structure of InsP(3)R and of the ABC domains docked into RyR are remarkably similar. The importance of the α-interface for activation of InsP(3)R and RyR is confirmed by mutagenesis and, for RyR, by disease-causing mutations. Binding of InsP(3) causes partial closure of the clam-like IBC, disrupting the β-interface and pulling the SD towards the IBC. This reorients an exposed SD loop ('hotspot' (HS) loop) that is essential for InsP(3)R activation. The loop is conserved in RyR and includes mutations that are associated with malignant hyperthermia and central core disease. The HS loop interacts with an adjacent NT, suggesting that activation re-arranges inter-subunit interactions. The A domain of RyR functionally replaced the SD in full-length InsP(3)R, and an InsP(3)R in which its C-terminal transmembrane region was replaced by that from RyR1 was gated by InsP(3) and blocked by ryanodine. Activation mechanisms are conserved between InsP(3)R and RyR. Allosteric modulation of two similar domain interfaces within an N-terminal subunit reorients the first domain (SD or A domain), allowing it, through interactions of the second domain of an adjacent subunit (IBC-β or B domain), to gate the pore.     

Elucidating the diel and seasonal calling behaviour of Elachistocleis matogrosso (Anura: Microhylidae)

ABSTRACT

Acoustic monitoring provides the opportunity to study ecological processes that are difficult to assess with traditional surveys. Elachistocleis matogrosso is an anuran species, described in 2010, for which limited biological information is available. This study investigated the calling activity of the species in the north-eastern portion of the Pantanal, Brazil, a wetland area with marked seasonality between the dry and wet seasons. The calling activity of E. matogrosso was monitored using automated digital recorders in combination with automated signal recognition software over two different annual cycles. The species was vocally active only during the wet season (October – April), with a peak in November-December during the 2013–2014 annual cycle and in February-March during the 2015–2016 annual cycle. The peak calling activity occurred at dusk. This species has nocturnal habits and an explosive breeding activity. The detection of the species was intermittent, which suggests that environmental predictors or site-specific conditions might play an important role in species detection. Moreover, this intermittent occupancy indicated that surveys that employ traditional field techniques would likely fail to detect this species. We describe an effective protocol for detecting E. matogrosso with acoustic monitoring, which requires recording during 20 days in February from 17:01 to 05:00. Our procedure would be easy to adapt to other anuran species, and it could be used for investigating new localities and assessing population changes over time.     

Forecasting with missing data: application to coastal wave heights

This paper presents a comparative analysis of linear and mixed models for short‐term forecasting of a real data series with a high percentage of missing data. Data are the series of significant wave heights registered at regular periods of three hours by a buoy placed in the Bay of Biscay. The series is interpolated with a linear predictor which minimizes the forecast mean square error. The linear models are seasonal ARIMA models and the mixed models have a linear component and a non‐linear seasonal component. The non‐linear component is estimated by a non‐parametric regression of data versus time. Short‐term forecasts, no more than two days ahead, are of interest because they can be used by the port authorities to notify the fleet. Several models are fitted and compared by their forecasting behaviour. Copyright © 1999 John Wiley & Sons, Ltd.     

项目引导型模式在电工电子实验教学中的应用

电工电子实验是电类人才教育的基础,直接影响着电类创新型人才的培养,而长期以来电工电子实验的教学模式因循守旧,已经不符合时代的需要。文中着力研究项目引导型实验教学模式在电工电子教学改革中的应用,通过改革内容、教学方法和评价体系三个方面的探索,证明了此模式对于提高学生的自主学习积极性、动手能力以及创新能力有着积极的效果。此项研究为电工电子实验教学改革以及创新型人才培养提供了一种有效途径,具有一定的现实意义。     

专业级录像机的自动磁迹跟踪重放技术

全面介绍目前专业级录像机的磁迹自动跟踪技术，特别重点讨论了新近发展的ＶＣＭ技术。     

Unravelling the dynamics of RNA degradation by ribonuclease II and its RNA-bound complex

RNA degradation is a determining factor in the control of gene expression. The maturation, turnover and quality control of RNA is performed by many different classes of ribonucleases. Ribonuclease II (RNase II) is a major exoribonuclease that intervenes in all of these fundamental processes; it can act independently or as a component of the exosome, an essential RNA-degrading multiprotein complex. RNase II-like enzymes are found in all three kingdoms of life, but there are no structural data for any of the proteins of this family. Here we report the X-ray crystallographic structures of both the ligand-free (at 2.44 A resolution) and RNA-bound (at 2.74 A resolution) forms of Escherichia coli RNase II. In contrast to sequence predictions, the structures show that RNase II is organized into four domains: two cold-shock domains, one RNB catalytic domain, which has an unprecedented alphabeta-fold, and one S1 domain. The enzyme establishes contacts with RNA in two distinct regions, the 'anchor' and the 'catalytic' regions, which act synergistically to provide catalysis. The active site is buried within the RNB catalytic domain, in a pocket formed by four conserved sequence motifs. The structure shows that the catalytic pocket is only accessible to single-stranded RNA, and explains the specificity for RNA versus DNA cleavage. It also explains the dynamic mechanism of RNA degradation by providing the structural basis for RNA translocation and enzyme processivity. We propose a reaction mechanism for exonucleolytic RNA degradation involving key conserved residues. Our three-dimensional model corroborates all existing biochemical data for RNase II, and elucidates the general basis for RNA degradation. Moreover, it reveals important structural features that can be extrapolated to other members of this family.