Formaldehyde-Schiff's reagent as a nucleolar strain.

The use of formaldehyde-Schiff's reagent as a nucleolar stain has been described. Using different digestion procedures, it was confirmed that the stain is specific for RNA. It can be suitably used as a nucleolar stain, particularly in plant materials after a short TCA extraction, which probably extracts the nonbound RNA.     

A convenient thin layer chromatographic method for the detection of cyclochlorotine and simatoxin, toxins from Penicillium islandicum Sopp

A rapid, convenient TLC method is described for detection of cyclochlorotine, a carcinogen produced by the commonly found storage mold Penicillium islandicum Sopp. This method has also led to the isolation of a new toxic metabolite, simatoxin.     

Occurrence of polyploidy and multinuclearity in the differentiating liver of chick embryo

Summary Increase in nuclear size in liver has been used as an index of polyploidy. It has long been considered that the occurrence of polyploidy and multinuclearity are characteristics of mammalian liver. The present study shows the occurrence of these phenomena in the liver of birds, so these features are not confined to mammals. 3 classes of nuclear size groups have been identified. The simultaneous occurrence of polyploidy and binuclearity indicates some sort of interrelationship between them.     

Enzymes of non-agglutinable vibrios and their possible role in the development of enterotoxic factor

Résumé Sachant que le facteur entérotoxique peut être développé dans les vibrions non-agglutinables par transfer animal, nous avons déterminé les activités enzymatiques (mucinase, protéase, lécithinase) de ces vibrions. Après ce transfert l'activité lécithinasique a augmenté, et cette activité est semblable à celle d'un virus (V. cholerae). Nous supposons que l'augmentation du facteur entérotoxique est causée par celle de l'activité de la lécithinase.

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Thanks are due to Dr.A. Mondal for help and Mr.Manzar Alam for his secretarial assistance.     

Stability of erythrocytic reduced glutathione and nicotinadenine dinucleotide phosphate in HbE-thalassaemia disease

Résumé La cause de l'instabilité du GSH érythrocytaire dans la thalassémie HbE est recherchée. Les activités des enzymes G-6-PD et GR sont normales ou élevées; le taux de NADP est diminué, d'où probablement vient l'instabilité du GSH.     

Water regulation in Barmer goat of the Rajasthan desert.

The different body fluid compartments in normally watered and 4-day water-deprived goats of Rajasthan desert, India, were measured in autumn. The goats maintained plasma volume and extracellular fluid volume, but lost gut and cell water considerably under the experimental conditions; indicating that the maintenance of the fluidity of the blood has priority over the body's other fluid requirements in this desert-adapted species during water deprivation.     

A convenient thin layer chromatographic method for the detection of cyclochlorotine and simatoxin,toxins fromPenicillium islandicum Sopp

Summary A rapid, convenient TLC method is described for detection of cyclochlorotine, a carcinogen produced by the commonly found storage moldPenicillium islandicum Sopp. This method has also led to the isolation of a new toxic metabolite, simatoxin.Acknowledgment. This work was supported by Food and Drug Administration, Washington, D.C. (Contract No. FDA-223-74-2209).     

Occurrence of 3-methoxy octadecanoic acid in yeast lipid

Summary The fatty acid composition of a new strain of the yeastRhodotorula glutinis, grown in molasses, has been studied and found to contain palmitic, stearic, oleic, linoleic and linolenic acids, and small amounts of other constituents. In addition, 3-methoxy octadecanoic acid has been shown to be present in the glycolipid fraction.Partly presented at the National Symposium on Natural Product Chemistry held on February 7–8, 1983 at Bose Institute, Calcutta (India).Acknowledgments. Authors are indebted to Prof. S.C. Bhattacharyya, and Prof. A.K. Barua, Department of Chemistry for providing facilities for this work.