Temperature modeling and control of Direct Methanol Fuel Cell based on adaptive neural fuzzy technology

Aiming at on-line controlling of Direct Methanol Fuel Cell （DMFC） stack, an adaptive neural fuzzy inference technology is adopted in the modeling and control of DMFC temperature system. In the modeling process, an Adaptive Neural Fuzzy Inference System （ANFIS） identification model of DMFC stack temperature is developed based on the input-output sampled data, which can avoid the internal complexity of DMFC stack. In the controlling process, with the network model trained well as the reference model of the DMFC control system, a novel fuzzy genetic algorithm is used to regulate the parameters and fuzzy rules of a neural fuzzy controller. In the simulation, compared with the nonlinear Proportional Integral Derivative （PID） and traditional fuzzy algorithm, the improved neural fuzzy controller designed in this paper gets better performance, as demonstrated by the simulation results.     

松花江哈尔滨段硅藻植物初报

本文报道了松花江哈尔滨段硅藻门植物,共发现100个分类单位,包括57种40变种3变型,分别隶属于2纲6目9科23属,初步分析了松花江哈尔滨段硅藻植物群落的种类组成及生态类型,大部分的硅藻属于普生种类,同时出现了一些喜酸种类如:Melosira granulate(Ehr．)Ralfs,Navicular viridula var．rostellata (Kutz．)Cl,Stauroneis anceps Ehr,Eunotia major W．Smith．等,还有少量的嗜碱性种类出现．     

基于跳跃滤波和时变参数估计的中国股市微观结构研究

为了更为有效地探究微观市场结构对股票价格的影响,本文在状态空间模型框架下,同时将交易方向、带方向的交易量、交易间隔、微观噪声以及跳跃因素引入状态方程和观测方程中,建立全面的市场微观结构模型,以反映各变量对交易价格序列产生的暂时性和永久性影响.然后,在采用基于粒子滤波的非参数离群点检测方法检测跳跃并对跳跃进行滤波的基础上,运用贝叶斯最小二乘方法对微观结构模型系数、波动率以及状态变量进行实时更新和估计,在此基础上分析跳跃日度分布、日内分布、个股跳跃和共同跳跃特征,并同时判断不同交易方向、交易量的订单对价格产生的影响.最后选取2015年股灾期间、沪市股票的逐笔数据作为研究样本进行实证研究.实证结果显示:我国股票高频时间序列跳跃和共同跳跃均存在日内季节性,且跳跃频率和大小与市场信息密切相关;买卖订单对交易价格及股票价值的影响并不是完全对称的,交易价格对于微观结构的敏感度、微观结构噪声也随市场波动而发生变化,熊市中都相对更高;考虑跳跃滤波并对参数进行实时估计可以提高价格对于交易的敏感度,能更好地捕捉市场微观结构在价格发现中的作用.     

固定化植物酯酶-显色剂传感膜的制备与性能

采用聚乙烯醇(PVA)与四乙氧基硅烷(TEOS)通过原位溶胶-凝胶法制备了PVA/SiO2有机/无机杂化溶胶,并将显色剂和植物酯酶分散于溶胶中,干燥成膜,得到一类透明度高、亲水-亲丙酮性可调、贮存性好的传感膜.该传感膜在乙酸-α-萘酯溶液中,会于可见光520 nm处出现一个新的吸收峰.传感膜在制备后的12天内活性明显下降,之后基本保持稳定,并且冷藏活性下降率要比室温保存的小很多.但不论是冷藏还是在室温下保存,被固定在杂化膜中的酶活稳定性都比在溶液状态下的明显提高.     

天花粉蛋白单克隆抗体杂交瘤细胞系的制备及鉴定

将小鼠骨髓瘤细胞（SP2／0细胞）与经TCS免疫过的小鼠脾细胞融合,融合后的细胞悬浮于HAT培养液中进行选择性培养,挑选可以分泌TCS单克隆抗体的细胞系,再经克隆化为单克隆细胞系。用间接酶联免疫吸附法检测此细胞系分泌单克隆抗体情况。经细胞融合和2次克隆化后,获得1株可以稳定分泌TCS单克隆抗体杂交瘤细胞系,ELISA检测结果为阳性。获得了可以稳定分泌天花粉蛋白单克隆抗体的杂交瘤细胞系,为TCS鉴定、应用奠定良好的基础。     

天花粉蛋白抗单纯疱疹病毒1型的实验研究

检测用基因工程方法制备的天花粉蛋白抗单纯疱疹病毒1型的活性。将单纯疱疹病毒1型在细胞系中培养．加入天花粉蛋白后,观察细胞病变情况,用酶联免疫吸附（ELISA）的方法检测病毒生长情况。结果显示,加入天花粉蛋白的细胞始终未见细胞病变,ELISA检测为阴性;阴性对照组细胞在第3d以后均出现单纯疱疹病毒1型感染典型细胞病变．ELISA检测结果为阳性;阳性对照组始终未见单纯疱疹病毒1型感染典型细胞病变,ELISA检测结果为阴性。天花粉蛋白在体外培养细胞系中可以抑制单纯疱疹病毒1型。     

Optimal control of multisolution ordinary differential equations in the absence of convexity

<正> In this paper,the authors study an optimal control problem governed by a class of multistateordinary differential equations in the absence of convexity.To overcome the difficulty that thecorresponding approximate optimal control problem may have no solution,relaxed controls are introduced.With the help of relaxation theory,Pontryagin's maximum principle for the optimal pairs ofthe original control problem is obtained.In the end of this paper,the authors discuss the applicationof the maximum principle by an example.     

构建以职业生涯需求为主导的就业指导体系

大学生处于职业生涯发展的探索期,其职业发展既有理想的成分,又不得不考虑现实条件的限制.因此,大学生的职业生涯发展有其特殊的需求.针对大学生职业生涯发展的特殊需求,开展全程化就业指导,是构建当前高校就业指导体系的有效策略.     

Assignment of the μ4-O5 atom in catalytic center for water oxidation in photosystem II

The detailed structure of catalytic center of water oxidation, Mn4Ca-cluster, in photosystem ⅡI (PSII) has been reported recently. However, due to the radiation damage induced by X-ray and the complexity of the Mn4Ca-cluster, the assignment of the μ4-O5 atom coordinated by three Mn and one Ca2+ ions is still lack of essential evidences. In this article, we synthesized one Mn complex containing two μ4-O atoms. It is found that the lengths of all μ4-O-Mn bonds in this Mn complex are in the range of 1.89-2.10 , which are significantly shorter than 2.40-2.61 distance of μ4-O5-Mn bonds in Mn4Ca-cluster observed in the crystal structure of PSII. In addition, DFT calculations have been carried out on the Mn4Ca-cluster. It is found that the O atom of μ4-O or μ4-OH always trends to deviate from the center position of four metal ions, resulting in unequal bond lengths of four μ4-4-M (M=Mn or Ca), which is obviously different with larger and nearly equal distances between μ4-O and four metal ions observed in the crystal structure. Based on these results, we suggest that the μ4-atom in Mn4Ca-cluster of PSII is unlikely to be a μ4-O, μ4-OH or μ4-OH2 , and its assignment is still an open question.     

新时期高校院所国家科技经费管理现状及对策

＂十二五＂时期,我国将进入以创新促转型,以转型促发展的新阶段,面对促进经济转型、创新发展模式的艰巨任务,高校院所是我国开展创新科学研究的一支重要力量。近年来,随着高等教育事业的蓬勃发展,高校院所承担的科研项目和筹措的科技经费均呈现大幅度增长,科技经费已成为高校院所重要的资金来源之一。如何提高国家科技经费的使用率、更好发挥国家财政科技经费的引导作用,是我国提高科技创新能力的一个重要任务。为提高国家财政科技经费资金使用效益,确保科研工作健康发展,需高度重视、加强高校院所国家财政科技经费管理及对策研究。