全文获取类型
收费全文 | 1375篇 |
免费 | 7篇 |
国内免费 | 16篇 |
专业分类
系统科学 | 9篇 |
丛书文集 | 4篇 |
教育与普及 | 31篇 |
理论与方法论 | 1篇 |
现状及发展 | 623篇 |
研究方法 | 74篇 |
综合类 | 631篇 |
自然研究 | 25篇 |
出版年
2017年 | 9篇 |
2015年 | 9篇 |
2012年 | 23篇 |
2011年 | 29篇 |
2010年 | 10篇 |
2009年 | 37篇 |
2008年 | 33篇 |
2007年 | 48篇 |
2006年 | 24篇 |
2005年 | 26篇 |
2004年 | 55篇 |
2003年 | 28篇 |
2002年 | 32篇 |
2001年 | 80篇 |
2000年 | 58篇 |
1999年 | 71篇 |
1992年 | 30篇 |
1991年 | 19篇 |
1990年 | 16篇 |
1989年 | 15篇 |
1988年 | 20篇 |
1987年 | 11篇 |
1986年 | 12篇 |
1985年 | 24篇 |
1984年 | 14篇 |
1983年 | 11篇 |
1982年 | 11篇 |
1980年 | 18篇 |
1979年 | 31篇 |
1978年 | 20篇 |
1977年 | 19篇 |
1976年 | 23篇 |
1975年 | 32篇 |
1974年 | 20篇 |
1973年 | 28篇 |
1972年 | 19篇 |
1971年 | 44篇 |
1970年 | 35篇 |
1969年 | 31篇 |
1968年 | 37篇 |
1967年 | 33篇 |
1966年 | 33篇 |
1965年 | 22篇 |
1964年 | 16篇 |
1960年 | 9篇 |
1959年 | 14篇 |
1958年 | 24篇 |
1957年 | 21篇 |
1956年 | 13篇 |
1948年 | 8篇 |
排序方式: 共有1398条查询结果,搜索用时 381 毫秒
41.
Angela M. Ortega-León Maricela Villagrán-Santa Cruz J. Jamie Zú?iga-Vega Raúl Cueva-del Castillo Fausto R. Méndez-de la Cruz 《西北部美国博物学家》2011,69(1)
Asynchronous reproduction is a common phenomenon in high-elevation populations of lizards from Central México. Sperm storage in the reproductive tract of females is the mechanism for making oocyte fertilization possible. Our study addresses questions related to functional oviductal sperm storage of females mating on different dates throughout the reproductive season. A population of Sceloporus mucronatus with copulation in the summer and ovulation in the fall was chosen for this experiment. Eleven females that copulated in the field during June and 13 females that copulated in captivity during August were maintained in the laboratory until parturition. The number of pregnant females and the litter sizes produced in each experimental group were indicative of the viability of the stored sperm. Sperm stored in the reproductive tract of females were able to fertilize eggs after 4 months. No significant differences were found in the number of pregnant females between the 2 experimental groups nor in the litter sizes that they produced. We found that the amount of time sperm were held in the female reproductive tract (ca. 3 months) had no effect on the capacity of sperm to fertilize eggs. Histological examination of 8 oviducts collected before the mating season eliminated the possibility of sperm storage from one year to the next. In this system, sperm retention could have evolved as a response mechanism to deal with the asynchrony between sexes in the reproductive cycles. However, we cannot rule out alternative hypotheses. 相似文献
42.
43.
Small G proteins are expressed ubiquitously in lymphoid cells and do not correspond to Bcl-2 总被引:2,自引:0,他引:2
The bcl-2 gene is consistently associated with t(14; 18) chromosomal translocations observed in a large fraction of human B-cell lymphomas. The t(14; 18) translocation results in deregulated expression of the bcl-2 gene and synthesis of inappropriately high levels of the Bcl-2 protein. Gene transfer studies suggest a role for Bcl-2 in cell survival, growth enhancement and oncogenic transformation. To test the suggestion that GTP-binding by Bcl-2 may mediate its biological effects we characterized the GTP-binding proteins in lymphoid cells expressing Bcl-2. Expression of several small GTP-binding proteins was found to be ubiquitous and did not vary with levels of Bcl-2. By using immunological, electrophoretic and cell-fractionation techniques, we separated Bcl-2 from G proteins of small relative molecular mass (Mr) and showed that it is incapable of binding GTP. Our results show that small Mr G proteins are widely expressed in lymphoid cells and that Bcl-2 is not a novel member of this GTP-binding protein family. 相似文献
44.
45.
The function and structure of the metal coordination sites within the glucocorticoid receptor DNA binding domain 总被引:54,自引:0,他引:54
L P Freedman B F Luisi Z R Korszun R Basavappa P B Sigler K R Yamamoto 《Nature》1988,334(6182):543-546
46.
We have developed technologies that simplify genomic library construction and screening, substantially reducing both the time and the cost associated with traditional library screening methods and facilitating the generation of gene-targeting constructs. By taking advantage of homologous recombination in Escherichia coli, we were able to use as little as 80 bp of total sequence homology to screen for a specific gene from a genomic library in plasmid or phage form. This method, called recombination cloning (REC), takes only a few days instead of the several weeks required for traditional plaque-lift methods. In addition, because every clone in the mouse genomic library we have constructed has a negative selection marker adjacent to the genomic insert, REC screening can generate gene-targeting vectors in one step, from library screening to finished construct. Conditional targeting constructs can be generated easily with minimal additional manipulation. 相似文献
47.
研究了硼钛复合纤维在拉伸过程中的行为,结果表明:复合纤维的变形是由纤维的弹性应变与基体的弹塑性应变复合迭加而成,并且在纤维与基体结合十分牢固,基体组元体积分数又较少的情况下,复合纤维表现为高强度,高弹性模量及低塑性,将试验结果同理论计算模型进行了比较,所得结果基本一致。 相似文献
48.
49.
以结构力学理论为依据,以有限元SAP5程序为计算手段,提出了一种直线振动筛侧板的动力学强度的计算方法,并分析了这类振动筛在侧板上产生裂纹的原因. 相似文献
50.
Excavations at the early Neolithic site of Jiahu in Henan Province, China have produced what may be the earliest complete, playable, tightly-dated multinote musical instruments. Jiahu was occupied from 7000 BC to 5700 BC, considerably antedating the well known Peiligang culture. Here we describe six exquisitely made complete flutes which were found in radiocarbon-dated excavation layers, along with fragments of perhaps 30 more. The flutes are made from the ulnae of the red-crowned crane (Grus japonensis Millen) and have 5, 6, 7 and 8 holes. The best preserved flute has been played and tonally analysed. In addition to early musical artefacts, the archaeological record at Jiahu contains important information on the very foundations of Chinese society. We describe the archaeological characteristics of the Jiahu site, details concerning its dating, its place in the prehistory of the Chinese Neolithic, the ethnicity of its population and the results of a tonal analysis of a nearly 9,000-year-old musical instrument found there. 相似文献