全文获取类型
收费全文 | 1429篇 |
免费 | 8篇 |
国内免费 | 16篇 |
专业分类
系统科学 | 9篇 |
丛书文集 | 4篇 |
教育与普及 | 31篇 |
理论与方法论 | 1篇 |
现状及发展 | 652篇 |
研究方法 | 80篇 |
综合类 | 650篇 |
自然研究 | 26篇 |
出版年
2017年 | 10篇 |
2015年 | 10篇 |
2012年 | 26篇 |
2011年 | 34篇 |
2010年 | 11篇 |
2009年 | 37篇 |
2008年 | 34篇 |
2007年 | 48篇 |
2006年 | 25篇 |
2005年 | 29篇 |
2004年 | 58篇 |
2003年 | 28篇 |
2002年 | 35篇 |
2001年 | 83篇 |
2000年 | 58篇 |
1999年 | 72篇 |
1992年 | 30篇 |
1991年 | 19篇 |
1990年 | 16篇 |
1989年 | 15篇 |
1988年 | 20篇 |
1987年 | 13篇 |
1986年 | 12篇 |
1985年 | 26篇 |
1984年 | 14篇 |
1983年 | 11篇 |
1982年 | 12篇 |
1980年 | 18篇 |
1979年 | 31篇 |
1978年 | 21篇 |
1977年 | 19篇 |
1976年 | 25篇 |
1975年 | 32篇 |
1974年 | 23篇 |
1973年 | 31篇 |
1972年 | 19篇 |
1971年 | 45篇 |
1970年 | 37篇 |
1969年 | 32篇 |
1968年 | 37篇 |
1967年 | 33篇 |
1966年 | 34篇 |
1965年 | 27篇 |
1964年 | 16篇 |
1960年 | 10篇 |
1959年 | 14篇 |
1958年 | 24篇 |
1957年 | 21篇 |
1956年 | 13篇 |
1948年 | 8篇 |
排序方式: 共有1453条查询结果,搜索用时 687 毫秒
61.
Summary T-2 toxin, a trichothecene metabolite of several Fusarium spp. causes depigmentation of dark mouse hair at the site of its application. Calcium pantothenate, though usually considered as antigreying factor, caused depigmentation at the site of its i.p. injections, at high concentration. 相似文献
62.
63.
Separation of rat liver mitochondrial amine oxidases 总被引:2,自引:0,他引:2
V Z Gorkin 《Experientia》1969,25(11):1142-1143
64.
65.
66.
67.
68.
HAb18G/CD147-mediated calcium mobilization and hepatoma metastasis require both C-terminal and N-terminal domains 总被引:4,自引:0,他引:4
Jiang JL Chan HC Zhou Q Yu MK Yao XY Lam SY Zhu H Ho LS Leung KM Chen ZN 《Cellular and molecular life sciences : CMLS》2004,61(16):2083-2091
HAb18G/CD147 is a heavily glycosylated protein containing two immunoglobulin superfamily domains. Our previous studies have indicated that overexpression of HAb18G/CD147 enhances metastatic potentials in human hepatoma cells by disrupting the regulation of store-operated Ca2+ entry by nitric oxide (NO)/cGMP. In the present study, we investigated the structure-function of HAb18G/CD147 by transfecting truncated HAb18G/CD147 fragments into human 7721 hepatoma cells. The inhibitory effect of HAb18G/CD147 on 8-bromo-cGMP-regulated thapsigargin-induced Ca2+ entry was reversed by the expression of either C or N terminus truncated HAb18G/CD147 in T7721C and T7721N cells, respectively. The potential effect of HAb18G/CD147 on metastatic potentials, both adhesion and invasion capacities, of hepatoma cells was abolished in T7721C cells, but not affected in T7721N cells. Release and activation of matrix metalloproteinases (MMPs), MMP-2 and MMP-9, were found to be enhanced by the expression of HAb18G/CD147, and this effect was abolished by both truncations. Thapsigargin significantly enhanced release and activation of MMPs (MMP-2 and MMP-9) in non-transfected 7721 cells, and this effect was negatively regulated by SNAP. However, no effects of thapsigargin or SNAP were observed in T7721 cells, and expression of HAb18G/CD147 enhanced secretion and activation of MMPs at a stable and high level. Taken together, these results suggest that both ectodomain and intracellular domains of HAb18G/CD147 are required to mediate the effect of HAb18G/CD147 on the secretion and activation of MMPs and metastasis-related processes in human hepatoma cells by disrupting the regulation of NO/cGMP-sensitive intracellular Ca2+ mobilization although each domain may play different roles.Received 1 April 2004; received after revision 15 June 2004; accepted 22 June 2004 相似文献
69.
Rusznák Z Pocsai K Kovács I Pór A Pál B Bíró T Szücs G 《Cellular and molecular life sciences : CMLS》2004,61(12):1532-1542
In this work, the distributions of some acid-sensitive two-pore-domain K+ channels (TASK-1, TASK-2 and TASK-3) were investigated in the rat and human cerebellum. Astrocytes situated in rat cerebellar tissue sections were positive for TASK-2 channels. Purkinje cells were strongly stained and granule cells and astrocytes were moderately positive for TASK-3. Astrocytes isolated from the hippocampus, cerebellum and cochlear nucleus expressed TASK channels in a primary tissue culture. Our results suggest that TASK channel expression may be significant in the endoplasmic reticulum of the astrocytes. The human cerebellum showed weak TASK-2 immunolabelling. The pia mater, astrocytes, Purkinje and granule cells demonstrated strong TASK-1 and TASK-3 positivities. The TASK-3 labelling was stronger in general, but it was particularly intense in the Purkinje cells and pia mater.Received 25 February 2004; received after revision 19 April 2004; accepted 28 April 2004 相似文献
70.
Ding H Wu X Boström H Kim I Wong N Tsoi B O'Rourke M Koh GY Soriano P Betsholtz C Hart TC Marazita ML Field LL Tam PP Nagy A 《Nature genetics》2004,36(10):1111-1116
PDGF-C is a member of the platelet-derived growth factor (PDGF) family, which signals through PDGF receptor (PDGFR) alphaalpha and alphabeta dimers. Here we show that Pdgfc(-/-) mice die in the perinatal period owing to feeding and respiratory difficulties associated with a complete cleft of the secondary palate. This phenotype was less severe than that of Pdgfra(-/-) embryos. Pdgfc(-/-) Pdgfa(-/-) embryos developed a cleft face, subepidermal blistering, deficiency of renal cortex mesenchyme, spina bifida and skeletal and vascular defects. Complete loss of function of both ligands, therefore, phenocopied the loss of PDGFR-alpha function, suggesting that both PDGF-A and PDGF-C signal through PDGFR-alpha to regulate the development of craniofacial structures, the neural tube and mesodermal organs. Our results also show that PDGF-C signaling is a new pathway in palatogenesis, different from, and independent of, those previously implicated. 相似文献