Retrograde transport of endocytosed Shiga toxin to the endoplasmic reticulum.

Shiga toxin and some other protein toxins that act on targets in the cytosol have previously been shown to enter the trans-Golgi network. Transport by this route may be necessary for translocation of the toxin to the cytosol and for intoxication, but it is not known whether the enzymatically active part of the toxins actually enters the cytosol from the trans-Golgi network. It has been suggested that such toxins are transported in a retrograde manner to the endoplasmic reticulum and that translocation occurs in this organelle, but retrograde transport of endocytosed material beyond the trans-Golgi network has never been demonstrated. Here we show that in butyric acid-treated A431 cells endocytosed Shiga toxin is not only transported to the trans-Golgi network, but also to all Golgi stacks, to the endoplasmic reticulum and to the nuclear envelope. Furthermore, butyric acid sensitizes the cells to Shiga toxin, which is consistent with the possibility that retrograde transport is required for translocation of the toxin to the cytosol.     

福州盆地南北港冲淤规律与洪涝灾害对策

本文探讨了福州盆地南北港冲淤规律，近年来北港洪水分流量呈上升趋势，洪峰对北港的压力加大，文中还对福州城防洪涝灾害的措施等方面作了研究。     

圣母圣心会呼和浩特传教述论

圣母圣心会在呼和浩特传教分晚清时期、民国时期。晚清时期，圣母圣心会传教士把传教的重点放在乡村，在呼和浩特无所作为。进入民国后，调整传教手段，迁址主教府，兴办医院、学校等公益事业。取得了一定效果。     

舰船·天安门·顶戴花翎——“‘洋务运动‘博物馆“东立面形象反思

通过对“洋务运动博物馆”东立面形象的反思 ,讨论了建筑形态设计中隐喻手法的运用 ;就此引申 ,探讨传统问题 ,指出正确理解传统与创新的四个方面。     

基于虚拟仪器的异步串行通信协议的检测方法

在分析基于RS－232C标准的串行异步通信原理的基础上，讨论了利用RVO2100虚拟数字示波器检测串行异步通信协议的方法，并与传统检测方法进行了比较。     

WebGIS实现方法与应用研究

在互联网高速发展的时代，基于WWW的地理信息系统应运而生。主要介绍了WebGIS的发展历程、技术特点和系统组织结构，并结合一个实际系统对其具体实现过程作了较详细的阐述。该系统构架在开发过程中取得了较好的效果。     

Influence of hydrochlorothiazide on the pain threshold and on the antinociceptive activity of morphine,in rats

Summary Hydrochlorothiazide, acutely injected in rats, has a weak analgesic activity per se and potentiates and prolongs the antinociceptive effect of morphine.This work was supported in part by grants from Consiglio Nazionale delle Ricerche, and by Ministero della Pubblica Istruzione, Roma.     

Resource Search in Unstructured Peer-to-Peer System Based on Multiple-Tree Overlay Structure

We propose a multiple-tree overlay structure for resource discovery in unstructured P2P systems. Peers that have similar interests or hold similar type of resources will be grouped into a tree-like cluster. We exploit the heterogeneity of peers in each cluster by connecting peers with more capacities closer to the root of the tree. The capacity of a peer can be defined in different ways （e.g. higher network bandwidth, larger disk space, more data items of a certain type etc.） according to different needs of users or applications.