Simple nanofabrication of a superhydrophobic and transparent biomimetic surface

This paper describes a simple fabrication method for creating superhydrophobic and transparent glass surfaces that mimic natural surfaces such as lotus leaves, moth eyes or cicada wings. Nanostructured glass surfaces were created by a combination of colloidal lithography and plasma etching. A colloidal mask was formed simply by the spin coating of the polystyrene beads and with modification of the interparticle distance between the beads. The etching of the glasses was conducted by CF4 plasma. Tower-shaped nanostructures at an aspect ratio of 1：4 were treated using fluoroalkylsilane selfassembled monolayers （SAMs） to obtain the hydrophobic surfaces. The treated glass surfaces showed superhydrophobicity with a water contact angle of around 150° and a hexadecane contact angle of around 110° Furthermore, the nanostructured glass was transparent to visible light.     

Cloning of a representative genomic library of the human X chromosome after sorting by flow cytometry

A library of 50,000 recombinants representative of the human X chromosome has been constructed. Human X chromosomes were physically separated using a fluorescence-activated cell sorter. The DNA was purified from the chromosomes, digested to completion with the restriction enzyme EcoRI and cloned into the phage lambda gtWES.lambda B. The X-derived nature of the recombinants was confirmed by hybridization to rodent/human cell line DNA containing only the human X chromosome. Such libraries will be particularly useful for the investigation of genetic diseases such as Duchenne muscular dystrophy, where the basic defect has not been elucidated, and of neoplasia, where several specific chromosomal anomalies, particularly for the leukaemias, have been identified.     

韩国国立古宫博物馆世宗朝自击漏复原(英文)

自动时报漏刻(自击宫漏)是宫中都工匠护军蒋英实奉世宗大王(李祹,1397—1450年;1418—1450年在位)之命于1433年制造的。从1434年7月1日开始它成为国家的标准计时仪器。对民众报时的午正、人定和罢漏就是根据这套计时仪器报的。它安装在景福宫的报漏阁里,因此后称报漏阁漏.景福宫是国家天文台"简仪台"所在地。它能够通过操控声音和视觉信号发生系统依朝鲜时代的二元化时制——即十二时辰和五更点法——自动报时。系统自动发出报时声同时显示指示,报出十二时、百刻,以及五更时刻。世宗大王创制的报漏阁自击漏成为模型在朝鲜(1392—1910年)经历过3次复制或修复——1433年的初创,1536年的再创以及1618年的重创。初创之后574年,2004年为了纪念韩国国立古宫博物馆在首尔开馆,文化财厅,博物馆与建国大学校和自击漏硏究会签订了自击漏复原计划,到2007年11月展示了复原的自击漏。复原计划是按照《世宗实录.报漏阁记》中的记载以及原器物的一些残余部分——包括1536年和1618年仪器的现存部分——进行的。用了一年半的时间复制漏器和自击装置。文章从机械学和功能方面的细节系统地描述了复原的过程,包括根据当时的教范《漏筹通义》的记载刻制十一支箭.《漏筹通义》是根据授时历和经过汉阳(当时朝鲜首都)地方时校正的《七政算内篇》编纂的。自击漏包括4个主要部分:(1)漏器部,通过安排三级播水壶,用于两个受水壶递水的计时壶来计时;(2)方木机构,通过在相应的时间落下相应的小铜丸将水平面转换到以数字显示的二元报时时刻标示信号;(3)铁丸放出机构,由复杂的杠杆系统操纵的机构放大动力报时机来操作。(4)视听觉报时部,由两个次一级结构组成。一个是十二时辰报时机部,包括一个钟和木制的小雕像系统,以不同次数的敲钟声和相应的木制的十二支神小雕像举出的时辰牌来报时。另一个是更点机,包括一面鼓、一面锣、以及小雕像,小雕像在不同的更点敲击出相应次数的锣鼓声以报更点。我们对已经失传几个世纪的铁丸作动人形机构的详细复制表明,蒋英实受到了阿拉伯铁丸利用报时法的启发,发明了丸落下时刻标时法(通过落丸来标示时刻的机械装置),并通过改进13世纪阿拉伯时计师al-Jazari的仪器来实现将动力传到对人形机构的推动中。另一方面,苏颂的《新仪象法要》,《元史》中描述的郭守敬的大明殿灯漏以及顺帝的宫漏可能影响了世宗朝的宫廷学者。总的来说,这次复原源于对当时的文献记载和遗物的重建,中国-阿拉伯钟表技术对我们也很有帮助。全部系统现在运行良好。我们的复原将有助于重建古代东亚、甚至阿拉伯的机械水钟。     

台湾在二氧化碳捕获及再利用中的技术研究状况

二氧化碳减量技术包括捕获、封存及再利用技术,是目前国际上亟待发展的减量技术的研究状况.阐述了近年来台湾致力于研发各项减量技术的研究状况,希望通过能源计划将针对化学吸收法、物理吸附法、超重力旋转填充床技术、化学循环程序、CO2转化技术等对台湾未来CO2减量提出一整合性技术.除了基础减量技术的研发之外,国科会通过能源计划整合产学研各界资源,共同开发CO2补获及再利用技术,并建立示范工厂,成为台湾CO2减量的重点技术平台.     

Fungitoxicity of m-fluorophenylalanine-containing peptides towards Pythium ultimum

The tripeptide L-m-fluorophenylalanyl-L-alanyl-L-alanine was much more fungitoxic towards Pythium ultimum than the dipeptide L-m-fluorophenylalanyl-L-alanine or m-fluorophenylalanine. The fungitoxicity of the tripeptide was reduced by L-alanyl peptides and phenylalanine, but not by other amino acids. In contrast, the fungitoxicity of m-fluorophenylalanine was unaffected by peptides, and was antagonized by several amino acids. These results suggest the effective delivery of m-fluorophenylalanine into the cell by a tripeptide carrier.     

Objective analysis of the topological organization of the primate cortical visual system.

The primate cortical visual system is composed of many structurally and functionally distinct areas, each receiving and sending about 10 projections from and to other cortical areas. The visual cortex is thus served by many cortico-cortical connections to form a network of considerable complexity. Thus the gross organization of this cortical processing system presents a formidable topological problem: although the spatial position of the areas in the brain is reasonably well established, the gross 'processing architecture' defined by the connections, is less well understood. Here I report an optimization approach that gives both qualitative and quantitative insight into the connectional topology of the primate cortical visual system. This approach supports suggestions that the system is divided into a dorsal 'stream' and a ventral 'stream' with limited cross-talk, that these two streams reconverge in the region of the principal sulcus (area 46) and in the superior temporal polysensory areas, that the system is hierarchically organized, and that the majority of the connections are from 'nearest-neighbour' and 'next-door-but-one' areas.     

Perinatal lethal osteogenesis imperfecta in transgenic mice bearing an engineered mutant pro-alpha 1(I) collagen gene

Substitutions of single glycine residues of alpha 1(I) collagen have previously been associated with the inherited disease osteogenesis imperfecta type II. Transgenic mice bearing a mutant alpha 1(I) collagen gene into which specific glycine substitutions have been engineered show a dominant lethal phenotype characteristic of the human disease, and demonstrate that as little as 10% mutant gene expression can disrupt normal collagen function.     

Molecular and genetic damage in humans from environmental pollution in Poland.

Extreme environmental pollution such as that found in the highly industrialized Silesian region of Poland has been associated with increased risk of cancer and adverse reproductive outcomes. Among the most prevalent carcinogenic and mutagenic air pollutants in Silesia are the polycyclic aromatic hydrocarbons (PAH) which are largely produced by industrial and residential combustion of coal. Molecular epidemiology aims to prevent disease by using biological markers to identify risks well before clinical onset to allow effective intervention. Here, we use a battery of biological markers to measure molecular and genetic damage in peripheral blood samples from residents of Silesia and from persons living in a rural, less polluted area of Poland. The results show that their exposure to environmental pollution is associated with significant increases in carcinogen-DNA adducts (PAH-DNA and aromatic adducts), in sister chromatid exchange including high-frequency cells, and in chromosomal aberrations as well as a doubling in the frequency of ras oncogene overexpression. We found that aromatic adducts on DNA were significantly correlated with chromosomal mutation, providing us with a molecular link between environmental exposure and a genetic alteration relevant to cancer and reproductive risk.     

Structure of Sindbis virus core protein reveals a chymotrypsin-like serine proteinase and the organization of the virion

Sindbis virus consists of a nucleocapsid core surrounded by a lipid membrane through which penetrate 80 glycoprotein trimers. The structure of the core protein comprising the coat surrounding the genomic RNA has been determined. The polypeptide fold from residue 114 to residue 264 is homologous to that of chymotrypsin-like serine proteinases with catalytic residues His 141, Asp 163 and Ser 215 of the core protein positioned as in other serine proteinases. The C-terminal tryptophan remains in the P1 substrate site subsequent to the autocatalytic cis cleavage of the capsid protein, thus rendering the proteinase inactive. Model building of the Sindbis core protein dimer shows that the nucleocapsid is likely to have T = 4 quasisymmetry.