A radiation hybrid map of the rat genome containing 5,255 markers.

A whole-genome radiation hybrid (RH) panel was used to construct a high-resolution map of the rat genome based on microsatellite and gene markers. These include 3,019 new microsatellite markers described here for the first time and 1,714 microsatellite markers with known genetic locations, allowing comparison and integration of maps from different sources. A robust RH framework map containing 1,030 positions ordered with odds of at least 1,000:1 has been defined as a tool for mapping these markers, and for future RH mapping in the rat. More than 500 genes which have been mapped in mouse and/or human were localized with respect to the rat RH framework, allowing the construction of detailed rat-mouse and rat-human comparative maps and illustrating the power of the RH approach for comparative mapping.     

蠕变断裂试验及初步研究(英文)

混凝土的蠕变断裂试验是一个相当困难的试验,以前还没有人成功地做过这样的试验。我们利用预裂的办法尽量减少混凝土的离散性进行了蠕变断裂试验,获得了蠕变断裂时间和荷载比关系,充分证实了篇变断裂的存在,以及发现在篇变断裂过程中伴随有微裂纹扩展。目前,我们用粘弹性理论蠕变断裂现象进行了初步分析,计算成果和试验非常好地呈现一致性。     

Relations phénétiques entre les familles d'Hamamelididae

Summary The phenetic relationships between the families of the Hamamelididae subclass were studied by using the following multifactorial analytical methods: principal components analysis (PCA) and reciprocal averaging (RA). The results obtained agree appreciably with Takhtajan's classification system.

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Remerciements. Nous remercions Mlle Sylvie Laliberté, Institut botanique de l'Université de Montréal, pour sa révision du manuscrit; M. Michel Labrecque, Institut botanique de l'Université de Montréal, pour avoir dessiné les figures; de même que M. André Bouchard, Jardin Botanique de Montréal et Institut botanique de l'Université de Montréal, de nous avoir permis d'utiliser les programmes dont il se sert pour ses travaux d'écologie.     

A simple procedure for removal of intra- and extra-tumoral macrophages from primary tumor cultures

Summary Improved growth of tumor cells in primary cell suspensions isolated from solid tumors can be achieved by addition of an antifungal agent, mycostatin, to the culture medium. Mycostatin inhibits the cytotoxicity of macrophages and also quickly detaches phagocytes without any apparent retardation of the growth of tumor cells. This method is particularly useful for obtaining cultures of lung tumor cells or cells from lung metastases of other tumors.This research was supported in part by grants CA-22839 and CA-28482 awarded by the National Cancer Institute, DHEW, USA. This is publication No. 406 from the Department of Basic and Clinical Immunology and Microbiology, Medical University of South Carolina.We thank Charles L. Smith for editorial assistance. Address correspondence to Y. Y., Department of Basic and Clinical Immunology and Microbiology, Medical University of South Carolina, 171 Ashley Avenue, Charleston, SC 29403, USA.     

Isolation of (−)-cryptosporiopsin,a chlorinated cyclopentenone fungitoxic metabolite fromPhialophora asteris f. sp.helianthi

Summary The (–)-enantiomer of cryptosporiopsin, a chlorinated cyclopentenone fungitoxic metabolite, was isolated fromPhialophora asteris f. sp.helianthi. Next to a comparable fungitoxic activity as shown by crypstosporiopsin, the product particularly inhibits growth ofSclerotinia sclerotiorum, an important pathogen on sunflower. Two further metabolites were tentatively identified as a stereoisomer of cryptosporiopsin and its dehydrated derivative.The authors are much indebted to Dr.G. M. Strunz (Canadian Forestry Service) for valuable suggestions and the generous gift of cryptosporiopsin and to the University of Utrecht for the part-time fellowship to one of us (Y.T.) given.     

Reduction of ferricytochrome c by human red cells

Summary Human red cells reduced extracellular ferricytochrome c to ferrocytochrome c under various conditions, suggesting that ferricytochrome c reducing systems are present at the outer surface of the red cell membrane.