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341.
P S Ohashi T W Mak P Van den Elsen Y Yanagi Y Yoshikai A F Calman C Terhorst J D Stobo A Weiss 《Nature》1985,316(6029):606-609
342.
Cloning, sequencing and expression of cDNA for a novel subunit of acetylcholine receptor from calf muscle 总被引:5,自引:0,他引:5
T Takai M Noda M Mishina S Shimizu Y Furutani T Kayano T Ikeda T Kubo H Takahashi T Takahashi 《Nature》1985,315(6022):761-764
The nicotinic acetylcholine receptor (AChR) from fish electric organ has a subunit structure of alpha 2 beta gamma delta, and this is thought to be also the case for the mammalian skeletal muscle AChR. By cloning and sequencing the complementary or genomic DNAs, we have previously elucidated the primary structures of all four subunits of the Torpedo californica electroplax and calf muscle AChR and of the alpha- and gamma-subunits of the human muscle AChR; the primary structures of the gamma-subunit of the T. californica AChR and the alpha-subunit of the Torpedo marmorata AChR have also been deduced elsewhere. We have now cloned DNA complementary to the calf muscle messenger RNA encoding a novel polypeptide (the epsilon-subunit) whose deduced amino-acid sequence has features characteristic of the AChR subunits and which shows higher sequence homology with the gamma-subunit than with the other subunits. cDNA expression studies indicate that the calf epsilon-subunit, as well as the calf gamma-subunit, can replace the Torpedo gamma-subunit to form the functional receptor in combination with the Torpedo alpha-, beta- and delta-subunits. 相似文献
343.
I. Kubo T. Matsumoto F. J. Hanke M. Taniguchi Y. Hayashi 《Cellular and molecular life sciences : CMLS》1985,41(11):1462-1463
Summary A possible new role for the flavonoid (–)-epicatechin (II) is described. It has no growth effects on its own, but when it is added to lettuce and rice seeds together with the known seedling growth inhibitor nagilactone E (I), the growth inhibitor activity ofI can cease and growth stimulation can be observed. 相似文献
344.
I-J epitopes are adaptively acquired by T cells differentiated in the chimaeric condition 总被引:1,自引:0,他引:1
I-J has been defined as a locus mapped in the murine major histocompatibility complex (MHC) which encodes serological markers found primarily on the surface of suppressor T cells (TS) and soluble suppressor factors (TSF). Recent studies have, however, revealed that there is no such specialized locus within the MHC at the DNA level. As the existence of I-J determinants at the protein level on functional T cells, T-cell clones and hybridomas has been confirmed by several serological and biochemical studies, this contradiction has raised serious arguments in the immunological community concerning the nature, origin and expression of I-J determinants. We have raised a number of monoclonal antibodies against the polymorphic structure of I-J molecules, and have studied the expression of I-J epitopes on T cells derived from irradiated bone marrow chimaeras in which stem cells of different genotype differentiated into T cells under the foreign host MHC environment. The results, presented here, indicate that I-J epitopes are not primarily determined by the MHC genes of the stem cells themselves, but are adaptively acquired by T cells differentiated in the chimaeric condition according to the environmental MHC phenotype. Thus, the serologically detectable I-J epitopes are found to be associated with inducible T-cell receptors recognizing self class II MHC antigens. 相似文献
345.
Sulfhydryl compounds such as reduced glutathione, cysteine and 2-mercaptopropionylglycine, a hepato-protective agent, activated Cu, Zn-superoxide dismutase purified from rat liver at low concentrations (below 10 microM). Furthermore we found evidence indicating that this activation is achieved by reducing Cu2+ present in the catalytic site of the dismutase, and thereby promoting the dismutation of superoxide anions. 相似文献
346.
The response of serum luteinizing hormone to glycine has been studied during the estrous cycle in adult female rats. I.p. administration of 200 mg of glycine significantly elevated serum luteinizing hormone levels at all stages of the estrous cycle. 相似文献
347.
348.
Location of functional regions of acetylcholine receptor alpha-subunit by site-directed mutagenesis 总被引:1,自引:0,他引:1
M Mishina T Tobimatsu K Imoto K Tanaka Y Fujita K Fukuda M Kurasaki H Takahashi Y Morimoto T Hirose 《Nature》1985,313(6001):364-369
The availability of cloned cDNAs encoding the four subunits of the Torpedo acetylcholine receptor, which can be expressed to make functional receptors in Xenopus oocytes, has made possible a detailed investigation of the functions of the different structural components of the receptor. The functional analysis of receptors with alpha-subunits altered at specific sites by site-directed mutagenesis of the cDNA has allowed the location of specific regions of the alpha-subunit molecule involved in acetylcholine binding and forming a transmembrane ionic channel. 相似文献
349.
Prednisolone, a water-soluble glucocorticoid hormone, suppressed the secretion of interleukin-1 beta from human peripheral blood mononuclear cells in culture. The prednisolone-induced suppression of the monokine release was dose-related and the half maximal response was observed at 0.1 nM. 相似文献
350.