排序方式: 共有56条查询结果,搜索用时 15 毫秒
41.
An outline of necrosome triggers 总被引:1,自引:1,他引:0
Tom Vanden Berghe Behrouz Hassannia Peter Vandenabeele 《Cellular and molecular life sciences : CMLS》2016,73(11-12):2137-2152
42.
Carroll and Chang have derived the symmetric CANDECOMP model from the INDSCAL model, to fit symmetric matrices of approximate
scalar products in the least squares sense. Typically, the CANDECOMP algorithm is used to estimate the parameters. In the
present paper it is shown that negative weights may occur with CANDECOMP. This phenomenon can be suppressed by updating the
weights by the Nonnegative Least Squares Algorithm. A potential drawback of the resulting procedure is that it may produce
two different versions of the stimulus space matrix. To obviate this possibility, a symmetry preserving algorithm is offered,
which can be monitored to produce non-negative weights as well.
This work was partially supported by the Royal Netherlands Academy of Arts and Sciences. 相似文献
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Sara M. Reekmans Thorsten Pflanzner Philip L. S. M. Gordts Simone Isbert Pascale Zimmermann Wim Annaert Sascha Weggen Anton J. M. Roebroek Claus U. Pietrzik 《Cellular and molecular life sciences : CMLS》2010,67(1):135-145
The proximal NPXY and distal NPXYXXL motifs in the intracellular domain of LRP1 play an important role in regulation of the
function of the receptor. The impact of single and double inactivating knock-in mutations of these motifs on receptor maturation,
cell surface expression, and ligand internalization was analyzed in mutant and control wild-type mice and MEFs. Single inactivation
of the proximal NPXY or in combination with inactivation of the distal NPXYXXL motif are both shown to be associated with
an impaired maturation and premature proteasomal degradation of full-length LRP1. Therefore, only a small mature LRP1 pool
is able to reach the cell surface resulting indirectly in severe impairment of ligand internalization. Single inactivation
of the NPXYXXL motif revealed normal maturation, but direct impairment of ligand internalization. In conclusion, the proximal
NPXY motif proves to be essential for early steps in the LRP1 biosynthesis, whereas NPXYXXL appears rather relevant for internalization. 相似文献
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Wortmann SB Vaz FM Gardeitchik T Vissers LE Renkema GH Schuurs-Hoeijmakers JH Kulik W Lammens M Christin C Kluijtmans LA Rodenburg RJ Nijtmans LG Grünewald A Klein C Gerhold JM Kozicz T van Hasselt PM Harakalova M Kloosterman W Barić I Pronicka E Ucar SK Naess K Singhal KK Krumina Z Gilissen C van Bokhoven H Veltman JA Smeitink JA Lefeber DJ Spelbrink JN Wevers RA Morava E de Brouwer AP 《Nature genetics》2012,44(7):797-802
Using exome sequencing, we identify SERAC1 mutations as the cause of MEGDEL syndrome, a recessive disorder of dystonia and deafness with Leigh-like syndrome, impaired oxidative phosphorylation and 3-methylglutaconic aciduria. We localized SERAC1 at the interface between the mitochondria and the endoplasmic reticulum in the mitochondria-associated membrane fraction that is essential for phospholipid exchange. A phospholipid analysis in patient fibroblasts showed elevated concentrations of phosphatidylglycerol-34:1 (where the species nomenclature denotes the number of carbon atoms in the two acyl chains:number of double bonds in the two acyl groups) and decreased concentrations of phosphatidylglycerol-36:1 species, resulting in an altered cardiolipin subspecies composition. We also detected low concentrations of bis(monoacyl-glycerol)-phosphate, leading to the accumulation of free cholesterol, as shown by abnormal filipin staining. Complementation of patient fibroblasts with wild-type human SERAC1 by lentiviral infection led to a decrease and partial normalization of the mean ratio of phosphatidylglycerol-34:1 to phosphatidylglycerol-36:1. Our data identify SERAC1 as a key player in the phosphatidylglycerol remodeling that is essential for both mitochondrial function and intracellular cholesterol trafficking. 相似文献
47.
Effect of temperature on in vitro proliferative activity of human umbilical vein endothelial cells 总被引:2,自引:0,他引:2
Human umbilical vein endothelial cells, skin fibroblasts, and retinal pigment epithelial cells are cultivated in medium supplemented with 15 to 20% serum in our laboratory. The effects of various incubation temperatures on the proliferation of these cells was examined. Our study shows that the mitogenic response of the endothelial cells to a change of temperature differed markedly from that of the fibroblasts and epithelial cells. Cultivation of human umbilical vein endothelial at 37°C required seeding densities as high as 1–2×104 cells/cm2, and yet resulted in a low growth rate and premature senescence. However, under the same culture conditions, but at 33°C, the proliferative capacity of these endothelial cells was potentiated. The results were striking; at 33°C the cells grew actively and the life span was extended. The number of cumulative population doublings increased fourfold compared with that for the same cells cultivated at 37°C. The inoculum size could be reduced, since at 33°C the endothelial cells were able to replicate at seeding densities as low as 20 cells/cm2. The cells serially subcultured at 33°C retained morphological features and specific immunological markers of endothelial cells. 相似文献
48.
R L Stone J Aimi B A Barshop J Jaeken G Van den Berghe H Zalkin J E Dixon 《Nature genetics》1992,1(1):59-63
We have examined the molecular basis of three cases of severe mental retardation with autistic features in one family. A point mutation in a purine nucleotide biosynthetic enzyme, adenylosuccinate lyase (ASL), segregates with the disorder. The affected children are homozygous for the point mutation while the parents and all four unaffected children are heterozygous. The point mutation is absent in control subjects. The point mutation results in a Ser413Pro substitution which leads to structural instability of the recombinant mutant enzyme, and this instability lowers ASL levels in lymphocytes. These observations suggest that the instability of ASL underlies the severe developmental disorder in the affected children, and that mutations in the ASL gene may result in other cases of mental retardation and autistic features. 相似文献
49.
XU Huaping WANG Yapei WANG Zhiqiang LIU Junqiu Mario Smet Wim Dehaen 《科学通报(英文版)》2006,51(19):2315-2321
Glutathione peroxidase (GPx) is a mammalian anti- oxidant seleno-enzyme that protects biomembranes and other cellular components from oxidative damage by catalyzing the reduction of a variety of hydroperoxides (ROOH), using glutathione (GSH) as the reduci… 相似文献
50.
According to the dorsoventral axis-inversion theory, protostomes (such as insects, snails and worms) are organized upside-down by comparison with deuterostomes (vertebrates), in which case their respective ventrally (belly-side) and dorsally (back-side) located nervous systems, as well as their midline regions, should all be derived from a common ancestor. Here we provide experimental evidence for such homology by showing that an orthologue of hedgehog, an important gene in midline patterning in vertebrates, is expressed along the belly of the larva of the limpet Patella vulgata. This finding supports the existence of a similar mechanism for the development of the midline of the nervous system in protostomes and deuterostomes. 相似文献