全文获取类型
收费全文 | 305篇 |
免费 | 0篇 |
国内免费 | 1篇 |
专业分类
系统科学 | 10篇 |
现状及发展 | 48篇 |
研究方法 | 26篇 |
综合类 | 204篇 |
自然研究 | 18篇 |
出版年
2017年 | 1篇 |
2013年 | 1篇 |
2012年 | 15篇 |
2011年 | 33篇 |
2010年 | 5篇 |
2008年 | 16篇 |
2007年 | 9篇 |
2006年 | 17篇 |
2005年 | 12篇 |
2004年 | 8篇 |
2003年 | 15篇 |
2002年 | 8篇 |
2001年 | 12篇 |
2000年 | 13篇 |
1999年 | 6篇 |
1998年 | 1篇 |
1997年 | 2篇 |
1996年 | 2篇 |
1993年 | 1篇 |
1992年 | 8篇 |
1991年 | 2篇 |
1990年 | 9篇 |
1989年 | 8篇 |
1988年 | 5篇 |
1987年 | 3篇 |
1986年 | 1篇 |
1985年 | 5篇 |
1984年 | 2篇 |
1983年 | 3篇 |
1982年 | 3篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1979年 | 3篇 |
1978年 | 7篇 |
1977年 | 4篇 |
1976年 | 4篇 |
1975年 | 6篇 |
1974年 | 4篇 |
1973年 | 7篇 |
1971年 | 4篇 |
1970年 | 12篇 |
1969年 | 5篇 |
1968年 | 5篇 |
1967年 | 4篇 |
1966年 | 6篇 |
1965年 | 5篇 |
1963年 | 1篇 |
1962年 | 1篇 |
排序方式: 共有306条查询结果,搜索用时 70 毫秒
21.
22.
Identifying the genes involved in polygenic traits has been difficult. In the 1950s and 1960s, laboratory selection experiments for extreme geotaxic behavior in fruit flies established for the first time that a complex behavioral trait has a genetic basis. But the specific genes responsible for the behavior have never been identified using this classical model. To identify the individual genes involved in geotaxic response, we used cDNA microarrays to identify candidate genes and assessed fly lines mutant in these genes for behavioral confirmation. We have thus determined the identities of several genes that contribute to the complex, polygenic behavior of geotaxis. 相似文献
23.
Visual abilities change over the visual field. For example, our ability to detect movement is better in peripheral vision than in foveal vision, but colour discrimination is markedly worse. The deterioration of colour vision has been attributed to reduced colour specificity in cells of the midget, parvocellular (PC) visual pathway in the peripheral retina. We have measured the colour specificity (red-green chromatic modulation sensitivity) of PC cells at eccentricities between 20 and 50 degrees in the macaque retina. Here we show that most peripheral PC cells have red-green modulation sensitivity close to that of foveal PC cells. This result is incompatible with the view that PC pathway cells in peripheral retina make indiscriminate connections ('random wiring') with retinal circuits devoted to different spectral types of cone photoreceptors. We show that selective cone connections can be maintained by dendritic field anisotropy, consistent with the morphology of PC cell dendritic fields in peripheral retina. Our results also imply that postretinal mechanisms contribute to the psychophysically demonstrated deterioration of colour discrimination in the peripheral visual field. 相似文献
24.
Areas of exploration for new hydrocarbons are changing as the hydrocarbon industry seeks new resources for economic and political reasons. Attention has turned from easily accessible onshore regions such as the Middle East to offshore continental shelves. Over the past ten years, there has been a marked shift towards deep-water continental margins (500-2,500 m below sea level). In these more hostile regions, the risk and cost of exploration is higher, but the prize is potentially enormous. The key to these endeavours is a quantitative understanding of the structure and evolution of the thinned crust and lithosphere that underlie these margins. 相似文献
25.
DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae 总被引:23,自引:0,他引:23
Heidelberg JF Eisen JA Nelson WC Clayton RA Gwinn ML Dodson RJ Haft DH Hickey EK Peterson JD Umayam L Gill SR Nelson KE Read TD Tettelin H Richardson D Ermolaeva MD Vamathevan J Bass S Qin H Dragoi I Sellers P McDonald L Utterback T Fleishmann RD Nierman WC White O Salzberg SL Smith HO Colwell RR Mekalanos JJ Venter JC Fraser CM 《Nature》2000,406(6795):477-483
26.
27.
Most ornithological literature for Utah reports the status of the Merlin ( Falco columbarius ) as rare or scarce. Only the most recently published checklists (Behle and Perry 1975, Behle et al. 1985) record it as uncommon. Likewise, a 1998 printed checklist (Utah Ornithological Society 1998) list it as uncommon. Also misrepresented in the literature are the status and distribution of the 3 rather distinct subspecies, each of which occupies dissimilar habitats during the breeding season. We made random observations of Merlins in northern Utah beginning in the 1950s and then studied them continuously between 1992 and 1997 in Utah County, Utah. During the 1992-1997 period, we were able to clearly allocate 95 males and 76 females to subspecies, including within our count 58 falcons trapped (some fitted with radio telemetry). The winter habitat in which they occurred was categorized as either urban (mainly dense residential area) or rural (agricultural lands, dairy farms, or scattered homes). There was a statistically significant ( X 2 F. c. columbarius frequenting urban areas 68% of the time, and prairie parkland-breeding F. c. richardsonii frequenting rural habitats 78% of the time. 相似文献
28.
Targets of homeotic gene control in Drosophila 总被引:18,自引:0,他引:18
29.
Genetic mapping and diagnosis of haemophilia A achieved through a BclI polymorphism in the factor VIII gene 总被引:1,自引:0,他引:1
Haemophilia A is the most common inherited bleeding disorder in man, affecting approximately 1 male in 10,000. The disease is caused by a deficiency in the gene for factor VIII, a component of the intrinsic coagulation pathway. Due to the broad range of clotting activity in normal and heterozygous females, it is often difficult to confirm the status of women at risk for carrying the disease. A genetic marker in the form of a restriction fragment length polymorphism (RFLP) within or tightly linked to the factor VIII gene would serve as a tag for the haemophilia gene, thus allowing both accurate carrier detection and improved, earlier prenatal diagnosis by chorionic villi sampling. The recent isolation of the factor VIII gene has allowed a search for RFLPs within the gene, and we report here the identification of a common polymorphism within the factor VIII gene, revealed by the restriction enzyme BclI, which can be used diagnostically in about 42% of all families. Although the disease haemophilia A has been mapped to the distal portion of Xq, the BclI RFLP makes possible higher-resolution genetic linkage mapping with respect to other polymorphic markers on this portion of the X chromosome. We have established close linkage of the factor VIII gene to several useful RFLP markers, including the highly informative marker St14. These markers should also be useful for prenatal diagnosis of haemophilia A and for detection of its carriers. 相似文献
30.
Alteration in crossbridge kinetics caused by mutations in actin 总被引:6,自引:0,他引:6
The generation of force during muscle contraction results from the interaction of myosin and actin. The kinetics of this force generation vary between different muscle types and within the same muscle type in different species. Most attention has focused on the role of myosin isoforms in determining these differences. The role of actin isoforms has received little attention, largely because of the lack of a suitable cell type in which the myosin isoform remains constant yet the actin isoforms vary. An alternative approach would be to examine the effect of actin mutations, however, most of these cause such gross disruption of muscle structure that mechanical measurements are impossible. We have now identified two actin mutations which, despite involving conserved amino acids, can assemble into virtually normal myofibrils. These amino-acid changes in actin significantly affect the kinetics of force generation by muscle fibres. One of the mutations is not in the putative myosin-binding site, demonstrating the importance of long-range effects of amino acids on actin function. 相似文献