Binding of 3,3',5'-triiodo-L-thyronine (rT3) to human serum proteins]

The rT3-binding and human serum proteins was directly studied with tracer doses of radioactive rT3. Polyacrylamide gel electrophoresis showed 125I-rT3 added to human serum was distributed among two proteins: albumin (carrying 57% of tracer rT3) and TBPA )22%). No binding was observed to TBG, protein binding T4.     

Diagnosis of the presence of meconium in amniotic fluid by dispersion curves of the longitudinal relaxations of protons]

Dispersion curves of the longitudinal relaxation T1 of protons in healthy amniotic fluid in a meconium solution are distinct at low Larmor frequencies (V0 less than 100 kHz). We are thus able to distinguish these fluids by T1 measurements in this range.     

ATP is a mediator of chemosensory transduction in the central nervous system

Extracellular signalling by the purine nucleotide ATP has long been associated with sensory function. In the periphery, ATP mediates nociception, mechanosensitivity, thermal sensitivity and O2 chemosensitivity. These processes share a common mechanism that involves the release of ATP to excite afferent fibres via activation of ionotropic P2X and/or metabotropic P2Y receptors. Chemosensors located in the brainstem are crucial for the maintenance of physiological levels of blood gases through the regulation of breathing. Here we show that an increase in pCO2 in the arterial blood triggers the immediate release of ATP from three chemosensitive regions located on the ventral surface of the medulla oblongata. Blockade of ATP receptors at these sites diminishes the chemosensory control of breathing, suggesting that ATP release constitutes a key step in central chemosensory transduction. These new data suggest that ATP, a phylogenetically ancient, unique and simple molecule, has been widely used in the evolution of afferent systems to mediate distinct forms of sensory transduction not only in the periphery but also within the central nervous system.     

Determination of pentoses

Riassunto Si descrive una modificazione del metodo diDische eBorenfreund per la determinazione dei pentosi, che raddoppia all'incirca la sensibilità. La modifica più importante consiste nel trattamento iniziale della soluzione zuccherina con HCl concentrato e nella riduzione a soli 5 min del tempo di immersione in bagno maria bollente dopo l'aggiunta del reattivo al floroglucinolo.     

Morphogenetic effects of follicle stimulating hormone: Effects on the synthesis of nuclear RNA's by chick embryos

Résumé L'hormone de maturation folliculaire (FSH), qui possède des propriétés morphogénétiques nettes, produit des effets différentiels sur la synthèse des ARN nucléaires chez des embryons de poule dans les différentes étapes de leur développement. Les expériences indiquent que lors du traitement par l'hormone, celle-ci peut stimuler la synthèse de différentes classes d'ARN, selon l'étape du développement.     

Effect of 5-azacytidine on developing mouse embryo

Zusammenfassung 5-Azacytidin wurde trächtigen Mäusen am 4.–6. und 11.–13. Tag verabreicht. Bei der ersten Gruppe zeigte sich eine völlige Resorption der Feutusse, während in der zweiten Gruppe hauptsächlich die Leber und das Nervengewebe geschädigt waren. In der ependymalen Zone werden die in Teilung befindlichen Zellen in der Metaphase gestoppt und unterliegen einer pyknotischen Degeneration.