Effect of chronic hypo and hypervitaminosis C on the brush border enzymes and the intestinal uptake of glucose and alanine

Summary Brush border sucrase and alkaline phosphatase activities are considerably enhanced in the intestine of ascorbic acid deficient guinea-pigs. Similar increase in the uptake of D-glucose and L-alanine also occurs in chronic vitamin C deficiency. However the permeability of D-glucose and L-alanine in the intestine of animals fed with large doses of vitamin C is severely depressed, with a reduction in the levels of sucrase and alkaline phosphatase activities.     

Über die Bindungsart der amidierten Reste derβ-Aminodicarbonsäuren in Eiweißstoffen

Summary Gliadin and chymotrypsinogen, respectively, were treated with alkaline sodium hypochlorite solution equivalent to the carbonamide groups present, and then hydrolysed by acid. The proteins treated with dilute alkaline solution have been assayed in control experiments for their content of N-terminal amino acids. The degradation products identified by paper chromatography seem to indicate that in chymotrypsinogen the amido groups of the amino dicarboxylic acids represent asparaginyl (Ia) and glutaminyl (Ib) residues. In gliadin, besides these residues, the presence of isoglutaminyl residues (IVb) also seems to be probable.     

Interaction of DEAE-dextran with mammalian cells cultivated in vitro

Zusammenfassung Wachstum, morphologische Eigenschaften und Karyotypie kultivierter L-Zellen wurden nach Applikation von DEAE-Dextran im Inkubations-medium untersucht. In einer Konzentration von 500 g/ml (was die Aufnahme von exogener DNS fördert) und bei einer Einwirkung während 30 min verändert DEAE-Dextran die untersuchten Eigenschaften nicht.