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41.
The prolyl isomerase Pin1 reveals a mechanism to control p53 functions after genotoxic insults 总被引:5,自引:0,他引:5
Zacchi P Gostissa M Uchida T Salvagno C Avolio F Volinia S Ronai Z Blandino G Schneider C Del Sal G 《Nature》2002,419(6909):853-857
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C. Di Ilio P. Sacchetta G. Del Boccio A. Muccini G. Polidoro 《Cellular and molecular life sciences : CMLS》1985,41(1):66-67
Summary The levels of GSH-related enzyme activities during pregnancy were determined. A significant increase in Selenium-dependent GSH peroxidase and GSH S-transferase E activity was observed. A concomitant increase in -glutamylcysteine synthetase was measured, which indicated an increased ability to synthesize the tripeptide. 相似文献
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Marini I Moschini R Del Corso A Mura U 《Cellular and molecular life sciences : CMLS》2005,62(24):3092-3099
The chaperone behaviour of bovine serum albumin was compared with that of α-crystallin. The chaperone activity was assessed
by measuring: (i) the ability to antagonize protein aggregation induced by heat; (ii) the capability to protect the activity
of thermally stressed enzymes and (iii) the effectiveness in assisting the functional recovery of chemically denatured sorbitol
dehydrogenase. Despite the lack of structural analogies, both proteins show several functional similarities in preventing
inactivation of thermally stressed enzymes and in reactivating chemically denatured sorbitol dehydrogenase. As with α-crystallin,
the chaperone action of bovine serum albumin appears to be ATP independent. Bovine serum albumin appears significantly less
effective than α-crystallin only in preventing thermally induced protein aggregation. A possible relationship between chaperone
function and structural organization is proposed. Together, our results indicate that bovine serum albumin acts as a molecular
chaperone and that, for its particular distribution, can be included in the extracellular chaperone family.
Received 29 August 2005; received after revision 23 September 2005; accepted 12 October 2005 相似文献
47.
Marini I Moschini R Del Corso A Mura U 《Cellular and molecular life sciences : CMLS》2005,62(5):599-605
-Crystallin, the major component of the vertebrate lens, is known to interact with proteins undergoing denaturation and to protect them from aggregation phenomena. Bovine lens sorbitol dehydrogenase (SDH) was previously shown to be completely protected by -crystallin from thermally induced aggregation and inactivation. Here we report that -crystallin, in the presence of the SDH pyridine cofactor NAD(H), can exert a remarkable chaperone action by favoring the recovery of the enzyme activity from chemically denaturated SDH up to 77%. Indeed, even in the absence of the cofactor, -crystallin present at a ratio with SDH of 20:1 (w:w) allows a recovery of 35% of the enzyme activity. The effect of ATP in enhancing -crystallin-promoted SDH renaturation appears to be both nonspecific and to not involve hydrolysis phenomena, thus confirming that the chaperone action of -crystallin is not dependent on ATP as energy donor.Received 28 October 2004; received after revision 22 December 2004; accepted 10 January 2005 相似文献
48.
Lens regeneration in adult newts is a classic example of how cells can faithfully regenerate a complete organ through the process of transdifferentiation. After lens removal, the pigment epithelial cells of the dorsal, but not the ventral, iris dedifferentiate and then differentiate to form a new lens. Understanding how this process is regulated might provide clues about why lens regeneration does not occur in higher vertebrates. The genes six-3 and pax-6 are known to induce ectopic lenses during embryogenesis. Here we tested these genes, as well as members of the bone morphogenetic protein (BMP) pathway that regulate establishment of the dorsal-ventral axis in embryos, for their ability to induce lens regeneration. We show that the lens can be regenerated from the ventral iris when the BMP pathway is inhibited and when the iris is transfected with six-3 and treated with retinoic acid. In intact irises, six-3 is expressed at higher levels in the ventral than in the dorsal iris. During regeneration, however, only expression in the dorsal iris is significantly increased. Such an increase is seen in ventral irises only when they are induced to transdifferentiate by six-3 and retinoic acid or by BMP inhibitors. These data suggest that lens regeneration can be achieved in noncompetent adult tissues and that this regeneration occurs through a gene regulatory mechanism that is more complex than the dorsal expression of lens regeneration-specific genes. 相似文献
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Riassunto Si descrive una semplice e rapida tecnica per trapiantare tumori sperimentali nel cervello di ratto, in modo non traumatizzante. Tra i tumori saggiati, l'epitelioma T8 uterino di Guèrin, si sviluppa nella totalità dei casi, con maggior rapidità rispetto ad altri tumori (Flexner-Jobling, Walker) ed in un tempo sensibilmente costante. Questo modello sperimentale viene utilizzato per studi di chemioterapia antitumorale. 相似文献