Cerebral edema in the rat with galactosamine induced severe hepatitis

Summary With D-galactosamine hydrochloride severe hepatitis was induced in rats and the water content of cerebrum, cerebellum and brain stem determined. The animals showed a parallel in cerebral water content and occurrence of cerebral symptoms.     

Effect of vagotomy upon the neurohistochemical and ultrastructural integrity of the inbuilt intrinsic nervous apparatus of the choledocho-duodenal junction

Summary The neurons of the choledocho-duodenal junction of the cat were shown to be neurohistochemically and morphologically independent of their extrinsic vagal connections. The effect of vagotomies upon the intrinsic nerve nets was also quite negligible.     

Interaction of cortisol and epinephrine in the regulation of leucine kinetics in man

To assess the interaction of the two major stress hormones epinephrine and cortisol in the regulation of leucine kinetics in man, epinephrine (50 ng/kg/min) was infused either alone or in combination with cortisol (2 micrograms/kg/min) into two groups of 6 postabsorptive normal male subjects during 180 min. Plasma leucine concentrations decreased by 28% (p less than 0.05) from baseline during epinephrine treatment (plasma levels 515 pg/ml); this was due to a decrease of leucine appearance (determined by 1-13C-leucine infusions) by 23% (p less than 0.025); leucine oxidation decreased by 29% (p less than 0.05). However, when plasma cortisol concentrations were elevated to supraphysiological levels (16.3 mumol/l) during epinephrine administration, the decreases of leucine plasma concentrations, appearance and oxidation were abolished. Plasma glucose and FFA concentrations were similarly elevated during both kinds of treatment. Since leucine appearance represents a measurement of total body protein breakdown and leucine disappearance into non-oxidative pathways reflects protein synthesis, the data indicate that plasma epinephrine concentrations during severe stress exert a protein anabolic effect in man which may counteract catabolic properties of elevated plasma cortisol.     

Incorporation of base analogues for detection of unusual base compositions in plant metaphase chromosomes (Vicia faba,Leguminosae)

Summary Bromodeoxyuridine and bromodeoxycytidine with fluorescence-plus-Giemsa staining resulted in similar patterns of asymmetric bands inVicia faba chromosomes. Limitations as to the use of methods for identification of A+T-and G+C-rich regions are discussed.Acknowledgements. We are grateful to Mr G. Versteegh. Groningen, for kindly providing us with a sample of BrdC and to Ms B. Hildebrandt for skilful technical assistance.     

Monocyte participation in connective tissue repair

Zusammenfassung Monocyten des strömenden Blutes könnenin vitro die morphologischen und funktionellen (Hydroxyprolinbildung) Kriterien von Fibrocyten erwerben. Die quantitative Bedeutung dieses Phänomens für den reparativen Bindegewebsaufbau wurde mit folgender Versuchsanordnung geprüft: Vergleichbare Inokulate von Monocyten (enthalten in der Leucocytenhaut des zentrifugierten Blutes) und von Fibrocyten (aus subcutanem Bindegewebe) von Kaninchen verschiedenen Geschlechts, wurden in Millipore-Kammern eingeschlossen und für 14–21 Tage in das Abdomen eines Kaninchens implantiert. Die Auszählung des Sex-Chromatins in den fibrocytären Kernen am Ende der Züchtungsperiode ergibt, dass ca. 50% monocytären Ursprunges sind. Es wird gefolgert, dass dieses Phänomen bei der eigentlichen Wundheilung noch bedeutungsvoller sein dürfte, da der Zustrom teilungsfähiger Monocyten in einem Wundgebiet kontinuierlich vor sich geht und nicht auf ein initiales Inokulum beschränkt ist.