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861.
Qingyu Kong Yifeng Shen Jun Zhuang Li Zhao Shixiong Qian Yufen Li Xu Li Yanghui Lin Ruifang Cai 《科学通报(英文版)》2002,47(4):284-291
Photofragmentation study of metal fullerides C60M
x
(M = Sm, Pt, Ni and Rh) by excimer laser ablation-TOF mass spectrometry shows that metallofullerenes C2n
M and C2n+1M formed in both the positive and negative ionic modes. The isotopic distributions of the metallofullerenes C2n
M and C2n+1M are consistent with the calculated spectra based on the natural abundance of isotopes of C and M, confirming the formation
of metallofullerenes. The metal atom is supposed to be incorporated into the network of the fullerene cage to replace one
carbon atom of the cage forming substitutional metallofullerene. Odd-numbered high carbon clusters are observed in our laser
ablation study of all the metal fullerides in the negative ion channel. Evolution of mass spectrum with irradiation laser
shots shows that the formation of the substitutionally doped fullerenes is closely related to the production of metal carbide
(MC). The structures, as well as formation mechanism, of metallofullerenes C2n+1M and C2n
M with even and odd numbers of total atoms respectively are discussed based on the structural optimization of the odd-numbered
high carbon clusters. 相似文献
862.
Intensity index series of Subtropical High over the Western Pacific was constructed according to modeling results by using of CCM3.6 atmospheric model with climatologically SST forcing for 30 years, and with observed SST forcing from 1900 to 2000. The index series constructed by modeling closely correlated to the observations for 1951-1999 (c.c. 0.72) and to the statistically reconstructed one for 1900-1950 (c.c. 0.51). The index series for 1900-1999 showed significant 80 a and 40 a periodicity. A minimum of persistence in seasonal variations of the index occurred in Autumn, which was called "Autumn Barrier". It may relate to the "Spring Barrier", found in the seasonal variations of SST over the Eastern Equatorial Pacific. The increasing trend shown in the index series for the 20th century may associate with the global warming caused by enhancement of greenhouse effect. 相似文献
863.
Construction of multivalent DNA vaccines forMycobacterium tuberculosis and its immunogenicity 总被引:1,自引:0,他引:1
The coding regions of Ag85B MPT-64, and ESAT-6 secreted proteins were cloned initially into the eukaryotic expression vector pJW4303, then transformed to E. coli Top 10 strain for plasmid DNA extraction and further analysis. Plasmids containing the right insertion were sequenced to confirm their identity. COS7 cells were transfected with a mixture containing serially diluted plasmid DNA encoding three secreted proteins and Lipofectin (Gibco). The supernatants and pellets prepared from various cell lines were run on SDS-PAGE gel and the expression of these proteins in COS7 cells were demonstrated by immunoblot using polyclonal or monoclonal antiserum of M.TBH37Rv. 21 days after first vaccination of C57BL-6 mice by all three recombinant eukaryotic expressing vectors, antibody titer for Ag85B reached 1∶3200. 21 days after second vaccination, the antibody titer reached 1∶102400. The highest antibody levels induced by multivalent vaccines after the second injection were equal to or even greater than the highest antibody levels of single DNA vaccine reported in literature after third injections. Antibody titer of MPT-64 was 1∶50 after the first injection and it reached 1∶200 after the second injection. No antigen-specific antibody against ESAT-6 was detected in sera harvested from immunized mice 21 days after both injections. Antigen-specific IFN-g level of Ag85B was 110 pg/mL while no antigen-specific IFN- g level of ESAT-6 and MPT-64 was detected even after third injections. To our knowledge, it is the first time that studies of polyvalent recombinant DNA vaccines against TB were carried out in C57BL-6 mice. Our results indicated that multiple DNA vaccines could be used to enhance protective responses against M.TB. 相似文献
864.
Assessment of blood and oxygen delivery to flaps of rhesus using near infrared steady-state spectroscopy 总被引:1,自引:0,他引:1
Near infrared spectroscopy has been considered as a potentially ideal noninvasive technique for the postoperative monitoring of plastic surgery. In this study, 6 flaps were raised on rhesus monkeys' forearms. Blood and oxygen delivery to these flaps was monitored following vascular occlusions and inhalation of pure oxygen. Optical fibers were adopted in the probe of the oximeter so that the cutaneous detection could be performed in reflectance mode. Different and repeatable patterns of changes were measured following vascular occlusions on flaps. It is clear that the near infrared spectroscopy is capable of postoperatively monitoring vascular problems in flaps, especially the venous problem. Near infrared spectroscopy showed high sensitivity to detect the dynamic changes in flaps induced by inhalation of pure oxygen in this study. The experimental results indicated that it was potential to assess tissue viability utilizing the dynamic changes induced by some noninvasive stimulation (such as inhaling pure oxygen). It may be a new assessing method that is rapid, little influenced by other factors and less inconvenient to patients. 相似文献
865.
866.
PFBC-CC发电技术的进展及创新发展 总被引:2,自引:0,他引:2
介绍和分析了增压流化床联合循环(PFBC-CC)发电技术在国外的进展以及国内由东南大学热能工程研究所作为技术依托单位承担的国家重点科技攻关项目--贾汪PFBC-CC中试电站的研究开发、建设和成功试验运行情况,并在此基础上探讨提出了发展部分气化循环床燃烧联合循环(PGCFB-CC)的创新发展思路。 相似文献
867.
依照湍动能理论及异形坯连铸的特点 ,建立了异形坯结晶器三维流场数学模型 ,运用该模型可分析不同水口参数及不同工艺参数条件下的流场分布 ,从而对连铸过程进行离线分析 ,确定最佳参数 ,并可作为在线控制模型的基础 相似文献
868.
本文介绍了一种基于MDT的组合夹具计算机辅助装配系统 ,简要介绍了组合夹具元件图库的建立 ,组合夹具的装配与定位误差分析。采用面向对象开发环境VC ++作为界面开发工具 ,利用ODBC技术实现数据库链接 ,采用Autodesk公司自身提供的ARX接口实现在MDT下应用程序的加载。 相似文献
869.
利用线-筒式荷电装置和高压窄脉冲电源来降低柴油机的微粒排放,分析了不同电源电压和脉冲频率下排气微粒的脱除效率,并在不同转速和负荷下进行了实验研究。研究结果表明,电源电压和脉冲频率是影响微粒脱除效率的两个主要因素,其中电源电压是最主要的影响因素,利用脉冲电晕放电降低柴油机的微粒排放效率明显的,是一种很有发展前途的控制尾气排放的新技术。 相似文献
870.
SIMPLE算法的一个新的改进方案 总被引:5,自引:1,他引:5
提出了SIMPLE算法的一个新的改进方案--MSIMPLE。该采用SIMPLEC中的方法对速度主程进行处理,同时对压力的确定采用SIMPLER中的方法来完成。通过对2个典型的流动传热问题(圆管突扩通道内的流动,正方形空腔内的自然对流)的计算,将该改进方案与SIMPLE,SIMPLEC,SEMPLER算法进行了初步对比,结果表明,改进方案有着更好的收敛特性,并且能节省数值计算的计算时间。 相似文献