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381.
The essential light chains constitute part of the active site of smooth muscle myosin 总被引:5,自引:0,他引:5
Myosin, a major contractile protein, characteristically possesses a long coiled-coil alpha-helical tail and two heads. Each head contains both an actin binding site and an ATPase site and is formed from the NH2-terminal half of one of the two heavy chains (relative molecular mass, Mr, 200,000) and a pair of light chains; the so-called regulatory and essential light chains of approximately Mr 20,000 each. Recently we have identified Trp 130 of the myosin heavy chain from rabbit skeletal muscle as an active-site amino-acid residue after labelling with a new photoaffinity analogue of ADP, N-(4-azido-2-nitrophenyl)-2-aminoethyl diphosphate (NANDP). Nonspecific labelling was eliminated by first trapping NANDP at the active site with thiol crosslinking agents. Exclusive labelling of the heavy chains with no labelling of the light chains agreed with previous findings that the heavy chains alone contain the actin-activated Mg-ATPase activity of rabbit skeletal myosin. Here we report similar photolabelling experiments with smooth muscle myosin (chicken gizzard) in which 3H-NANDP is trapped at the active site with vanadate and which show that both the heavy chains and the essential light chains are labelled. The results indicate that both chains contribute to the ATP binding site and represent the first direct evidence for participation of the essential light chains in the active site of any type of myosin. 相似文献
382.
Absence of cooperative haemoglobin-oxygen binding in Sphenodon, a reptilian relict from the Triassic
It is generally accepted that the sigmoidal nature of the haemoglobin-oxygen dissociation curve (ODC) is necessary for efficient oxygen transport in terrestrial vertebrates because it allows large volumes of oxygen to be bound or released for relatively small changes in the partial pressure of oxygen (PO2) in the blood. Furthermore, the amount of oxygen to tissues is increased by hydrogen ions produced from the dissociation of carbon dioxide in solution. The generality of these key features of cooperative oxygen binding and the Bohr effect holds for reptiles, birds and mammals, including representatives with special respiratory requirements for diving, burrowing and living at high altitude. Sphenodon punctatus is the sole surviving representative of the ancient order of 'beakhead' reptiles (order Rhynchocephalia) which were once widely distributed during the Triassic period before the spectacular radiation of dinosaur faunas. We have now investigated the oxygen transporting properties of blood from Sphenodon and find that the ODC is hyperbolic, with a high affinity for oxygen and very small Bohr effect. This combination of characteristics is unique among terrestrial vertebrates and accords with a low demand for oxygen and limited scope for aerobic activity. 相似文献
383.
The Tetrahymena ribozyme acts like an RNA restriction endonuclease 总被引:41,自引:0,他引:41
A shortened form of the Tetrahymena self-splicing ribosomal RNA intervening sequence acts as an endoribonuclease, catalysing the cleavage of large RNA molecules by a mechanism involving guanosine transfer. The sequence specificity approaches that of the DNA restriction endonucleases. Site-specific mutagenesis of the enzyme active site alters the substrate sequence specificity in a predictable manner, so that endoribonucleases can be synthesized to cut at a variety of tetranucleotide sequences. 相似文献
384.
M. M. Hanumante T. A. Butler M. Fingerman 《Cellular and molecular life sciences : CMLS》1984,40(7):740-741
Summary 5-Hydroxytryptamine (5-HT) antibodies inhibit red pigment dispersion in the fiddler crab,Uca pugilator. This observation supports the hypothesis that 5-HT stimulates release of red pigment-dispersing hormone.The 5-HT antibody preparation was a generous gift from Dr S. Spector, Roche Institute of Molecular Biology, Nutley, New Jersey. 相似文献
385.
M. V. Novotny T. -M. Xie S. Harvey D. Wiesler B. Jemiolo M. Carmack 《Cellular and molecular life sciences : CMLS》1995,51(7):738-743
Two male mouse pheromones, 3,4-dehydro-exo-brevicomin (DHB) and 2-sec-butyldihydrothiazole (SBT), are chiral molecules which were previously tested in their respective bioasays as racemic mixtures. The focus of this study has been to determine the absolute configuration of their natural forms and its relation to stereospecific biological action. DHB was established as the R,R-enantiomer possessing biological activity. Due to an extremely easy racemization of SBT under very mild conditions, enantioselectivity of its transmission and its action at the receptor site appear to be of secondary importance. 相似文献
386.
Effector T lymphocyte line cells migrate to the thymus and persist there 总被引:15,自引:0,他引:15
Y Naparstek J Holoshitz S Eisenstein T Reshef S Rappaport J Chemke A Ben-Nun I R Cohen 《Nature》1982,300(5889):262-264
387.
388.
Summary Melatonin, in concentrations up to 10–3
M, showed no effect on mitosis in cultures of HeLa or KB cells. However, when melatonin at 10–4
M was preincubated with HeLa cells prior to addition of 10–7
M colchicine, a reduction in the mitotic index, in comparison to colchicine alone, was observed.This work was supported by PHS Grant No. CA 16425. 相似文献
389.
Retroviral antigens on gs- chf- leukocytes 总被引:1,自引:0,他引:1
It has recently been suggested that the endogenous retroviruses present in many different species might be involved during stimulation of the immune system of their hosts. We have now studied the expression of two avian retroviral antigens p27 and gp85 in chicken lymphoid cells by indirect immunofluorescence (IIF) and by complement-dependent microcytotoxicity (CDM). We have now found that these viral antigens are expressed in peripheral blood leukocytes of adults and embryos and in splenic and bursal lymphocytes of Spafas gs- chf- chickens but they are not expressed in fibroblasts cultured from the feather follicles of the same individual adult birds nor in fibroblasts cultured from embryos of the same flock. The differential expression of viral antigens in leukocytes may be related to a specific property or function of these cells. 相似文献
390.
Summary 4000 rads of mixed gamma neutron radiation administered to rhesus monkeys released a significant amount of histamine into their circulation. When the monkeys were treated with a mast-cell histamine depleter (compound 48/80) for 4 days and then irradiated, no increase in circulating histamine was seen. When 48/80 was given 20 min after irradiation, only a slight increase in histamine was seen, indicating that 4000 rads had released most of the mast-cell histamine. 相似文献