应用最优化：工程系统的表述与算法

将一个问题用数值表示其出发点是抓住问题本身的直观思想并且转换为恰当的数学语言。本书采用逐步深入的方法刻划如何表述数学问题，通过这种描述人们可以应用现有的软件去解决它。作者根据数值问题的不同类型论述了求解的技术，通过许多来自工程技术领域的典型实例详细地解说了问题由直观叙述到易于控制的数值形式的转换过程，特别包含了许多有效算法。     

Calibration of the HSPF model with a new coupled FTABLE generation method

The hydrological simulation program FORTRAN (HSPF) is a comprehensive watershed model designed to simulate water quantity and quality processes.It is used widely throughout the world.Calculating discharge from a stream reach by HSPF is based mainly on function tables (FTABLEs),which describe the relationship between surface area,stream stage,volume,and discharge.The traditional method of FTABLE generation (using general stream cross-sections and the Manning equation or other discharge relationships) can be time-consuming and inaccurate when there are limited calibration stations and survey data.Recent insights into generalized coupled stage/storage and stage/discharge relationships,however,offer the potential to improve both the speed and accuracy of compiling FTABLEs and thus HSPF models.This study tested the application of these insights in generating FTABLEs.The study domain included 18 gauging stations in a coastal plain environment on the Peace River watershed in West-Central Florida.In the calibration of this model,wetlands were treated as reaches in HSPF with storage-attenuation characteristics.Over the simulation period,the modeled daily flow and stage correlated well with observed data at all ganging stations along the Peace River.The Nash-Sutcliffe efficiency and correlation coefficient computed using observed and simulated daily flows were 0.90 and 0.91,respectively,at the Peace River outlet.Rigorous testing found that the new system for calibrating the FTABLEs improved the model predictive ability and efficiency.Uncertainty in un-calibrated station performance was also reduced.The model parameters obtained in this study could potentially serve as reference values for model application in similar climatic regions.They may also have particular relevance in disaster management,as the simulation period extended over the hurricane season of 2004,and the model domain was within a high hurricane impact zone.The possible effects of hurricanes on discharge are also briefly discussed.     

Direct expression in Escherichia coli of a DNA sequence coding for human growth hormone.

DNA coding for human growth hormone was constructed by using chemically synthesised DNA in conjunction with enzymatically prepared cDNA. This 'hybrid' gene was expressed in Escherichia coli under the control of the lac promoter. A polypeptide was produced having the size and immunological properties characteristic of mature human growth hormone.     

Failure of hemicholinium-3 to inhibit the uptake of3H-choline in mouse brain in vivo

Zusammenfassung Es wird die Wirkung von Hemicholinium-3-dibromid und Troxopyrrolidinium chlorid auf die Aufnahme von [³H]Cholin im Gehirn der Maus studiert. Beide Substanzen hatten keine Wirkung, und es ist wahrscheinlich, dass der Mechanismus der Cholinaufnahme in das Gehirn vom dem der Cholinaufnahme in die cholinergischen Nervendigungen verschieden ist.     

Vascular effects of periarterial mesenteric nerve stimulation after adrenergic neurone blockade

Resume La stimulation électrique du plexus nerveux périartérial mésentérique provoque une vasodilatation résistante à l'atropine de la couche vasculaire mésentérique chez le chat anesthésié après blocage des neurones adrénergiques.     

Polyglutamine expansion of huntingtin impairs its nuclear export

Proteins with polyglutamine (polyQ) expansions accumulate in the nucleus and affect gene expression. The mechanism by which mutant huntingtin (htt) accumulates intranuclearly is not known; wild-type htt, a 350-kDa protein of unknown function, is normally found in the cytoplasm. N-terminal fragments of mutant htt, which contain a polyQ expansion (>37 glutamines), have no conserved nuclear localization sequences or nuclear export sequences but can accumulate in the nucleus and cause neurological problems in transgenic mice. Here we report that N-terminal htt shuttles between the cytoplasm and nucleus in a Ran GTPase-independent manner. Small N-terminal htt fragments interact with the nuclear pore protein translocated promoter region (Tpr), which is involved in nuclear export. PolyQ expansion and aggregation decrease this interaction and increase the nuclear accumulation of htt. Reducing the expression of Tpr by RNA interference or deletion of ten amino acids of N-terminal htt, which are essential for the interaction of htt with Tpr, increased the nuclear accumulation of htt. These results suggest that Tpr has a role in the nuclear export of N-terminal htt and that polyQ expansion reduces this nuclear export to cause the nuclear accumulation of htt.     

Disruption of Bardet-Biedl syndrome ciliary proteins perturbs planar cell polarity in vertebrates

The evolutionarily conserved planar cell polarity (PCP) pathway (or noncanonical Wnt pathway) drives several important cellular processes, including epithelial cell polarization, cell migration and mitotic spindle orientation. In vertebrates, PCP genes have a vital role in polarized convergent extension movements during gastrulation and neurulation. Here we show that mice with mutations in genes involved in Bardet-Biedl syndrome (BBS), a disorder associated with ciliary dysfunction, share phenotypes with PCP mutants including open eyelids, neural tube defects and disrupted cochlear stereociliary bundles. Furthermore, we identify genetic interactions between BBS genes and a PCP gene in both mouse (Ltap, also called Vangl2) and zebrafish (vangl2). In zebrafish, the augmented phenotype results from enhanced defective convergent extension movements. We also show that Vangl2 localizes to the basal body and axoneme of ciliated cells, a pattern reminiscent of that of the BBS proteins. These data suggest that cilia are intrinsically involved in PCP processes.