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Diep CQ Ma D Deo RC Holm TM Naylor RW Arora N Wingert RA Bollig F Djordjevic G Lichman B Zhu H Ikenaga T Ono F Englert C Cowan CA Hukriede NA Handin RI Davidson AJ 《Nature》2011,470(7332):95-100
Loss of kidney function underlies many renal diseases. Mammals can partly repair their nephrons (the functional units of the kidney), but cannot form new ones. By contrast, fish add nephrons throughout their lifespan and regenerate nephrons de novo after injury, providing a model for understanding how mammalian renal regeneration may be therapeutically activated. Here we trace the source of new nephrons in the adult zebrafish to small cellular aggregates containing nephron progenitors. Transplantation of single aggregates comprising 10-30 cells is sufficient to engraft adults and generate multiple nephrons. Serial transplantation experiments to test self-renewal revealed that nephron progenitors are long-lived and possess significant replicative potential, consistent with stem-cell activity. Transplantation of mixed nephron progenitors tagged with either green or red fluorescent proteins yielded some mosaic nephrons, indicating that multiple nephron progenitors contribute to a single nephron. Consistent with this, live imaging of nephron formation in transparent larvae showed that nephrogenic aggregates form by the coalescence of multiple cells and then differentiate into nephrons. Taken together, these data demonstrate that the zebrafish kidney probably contains self-renewing nephron stem/progenitor cells. The identification of these cells paves the way to isolating or engineering the equivalent cells in mammals and developing novel renal regenerative therapies. 相似文献
894.
Along the margins of playas in northwestern Nevada, a salt-tolerant plant community occupies mounds that dot a largely unvegetated landscape. In this environment we studied soil development and plant-soil relationships. The mounds, averaging 0.3 m in height, are occupied by the shrubs Allenrolfea occidentalis (iodine bush), Sacrobatus vermiculatus (black greasewood), and Atriplex lentiformis spp. torreyi (Torrey saltbush). Distichlis spicata (desert saltgrass) is the only herbaceous plant occupying this community. Soil salinity decreases with depth in this environment, and content of aqueous-extractable solutes is significantly influenced by site-specific vegetation. Content of silt, clay, and salt in mound surface horizons suggests a chronosequence of mound formation, with the youngest at the barren playa interface and the oldest at the upland vegetation border. Plant demography and mound soil stratigraphy suggest that a pulse of plant recruitment and mound building occurred during a time of neoglacial cooling. As a substrate for plant recruitment, mounds have a limited lifespan because deposition of eolian-transported salts and geochemical cycling by plants quickly render them too saline for seed germination. The apparent productivity of mound formation precludes definitive conclusions regarding those mound characteristics favorable for plant recruitment and survivorship. 相似文献
895.
Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} New records for Myotis californicus, M. evotis, M. leibii, M. lucifugus, M. thysanodes, M. volans, M. yumanensis, Lasionycteris noctivagans, Pipistrellus hesperus, Eptesicus fuscus, Lasiurus cinereus, Plecotus townsendii, and Antrozous pallidus and their habitat occurrence in northwestern Colorado are reported. Mortality of 27 bats of six species trapped in an oil sludge pit is described. 相似文献
896.
Seasonal wildlife observations were made along transects on 2 pastures conservatively grazed (36% use of perennial grasses) and 2 pastures moderately grazed (47% use of perennial grasses) in south central New Mexico in non-drought (1997) and drought years (1998). Experimental pastures were similar in soils, terrain, spacing of watering points, and brush cover. Average ecological condition score for the conservatively grazed pastures was 60% compared with 64% for moderately grazed pastures. Throughout the study total standing vegetation understory herbage levels were higher ( P P > 0.05) between conservatively and moderately grazed pastures. Black-tailed jackrabbit ( Lepus californicus ) sightings were higher ( P Antilocapra americana ), scaled quail ( Callipepla squamata ), mourning doves ( Zenaida macroura ), and desert cottontails ( Sylvilagus auduboni ) showed no differences ( P > 0.05) between conservatively and moderately grazed pastures. Dry conditions in 1998 depressed total wildlife sightings by > 50% compared to 1997. Both songbird and gamebird (particularly mourning dove) sightings were severely reduced in the dry compared to wet year ( P < 0.05). Our results are consistent with Nelson et al. (1997) that livestock grazing at intermediate levels had no effect on most Chihuahuan Desert upland wildlife species, and that drought years severely depress wildlife sightings. 相似文献
897.
Systemic Practice and Action Research - 相似文献
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Ruban AV Berera R Ilioaia C van Stokkum IH Kennis JT Pascal AA van Amerongen H Robert B Horton P van Grondelle R 《Nature》2007,450(7169):575-578
Under conditions of excess sunlight the efficient light-harvesting antenna found in the chloroplast membranes of plants is rapidly and reversibly switched into a photoprotected quenched state in which potentially harmful absorbed energy is dissipated as heat, a process measured as the non-photochemical quenching of chlorophyll fluorescence or qE. Although the biological significance of qE is established, the molecular mechanisms involved are not. LHCII, the main light-harvesting complex, has an inbuilt capability to undergo transformation into a dissipative state by conformational change and it was suggested that this provides a molecular basis for qE, but it is not known if such events occur in vivo or how energy is dissipated in this state. The transition into the dissipative state is associated with a twist in the configuration of the LHCII-bound carotenoid neoxanthin, identified using resonance Raman spectroscopy. Applying this technique to study isolated chloroplasts and whole leaves, we show here that the same change in neoxanthin configuration occurs in vivo, to an extent consistent with the magnitude of energy dissipation. Femtosecond transient absorption spectroscopy, performed on purified LHCII in the dissipative state, shows that energy is transferred from chlorophyll a to a low-lying carotenoid excited state, identified as one of the two luteins (lutein 1) in LHCII. Hence, it is experimentally demonstrated that a change in conformation of LHCII occurs in vivo, which opens a channel for energy dissipation by transfer to a bound carotenoid. We suggest that this is the principal mechanism of photoprotection. 相似文献