Approach to a quantitative differentiation between the respiratory effects evoked from the lung stretch and the lung deflation receptors during thoracic compression

Zusammenfassung Die beiden Annahmen, dass sich bei Thoraxkompression die Verminderung der Impulssequenz der Lungendehnungsrezeptoren analog zum Lungenentdehnungsreflex auswirke, und dass sich dessen inspiratorische Atmungseffekte zu jenen des Lungenkollapsreflexes addieren, erscheinen gerechtfertigt. Diese Annahmen ermöglichen eine quantitative Differenzierung zwischen dem respiratorischen Einfluss der Lungendehnungs-und Lungenkollapsrezeptoren bei Thoraxkompression. Damit können die auf die Erregung der Lungenkollapsrezeptoren zu beziehenden Atmungsveränderungen (f,I/E) erstmals isoliert und quantitativ dargestellt werden.

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Presented at the 2nd Meeting of the Swiss Physiological Society, Zürich, 8 November 1969.     

Expression of a transposable antibiotic resistance element in Saccharomyces

Some eukaryotic genes can be expressed in bacteria but there are few examples of the expression of prokaryotic genes in eukaryotes. Antibiotic G418 is a 2-deoxystreptamine antibiotic that is structurally related to gentamicin but has inhibitory activity against a much wider variety of pro- and eukaryotic organisms. In bacteria, resistance to G418 can be determined by several plasmid-encoded modifiying enzymes and, in view of the broad spectrum of activity of G418, we considered that this antibiotic might be useful as a selective agent for the introduction of these antibiotic resistance genes into a eukaryotic organism such as Saccharomyces cerevisiae. Additional impetus for these experiments came from the knowledge that certain of the G418-resistance determinants in bacteria are carried on transposable elements; a study of the properties of these elements in eukaryotes would be intriguing.