Efficient expression of human factor Ⅸ cDNA in liver mediated by hydrodynamics-based plasmid administration

The transfection of plasmid DNA into mammalian cells is an indispensable tool in the study of gene transfer and gene function. Since the original report in 1990 on the successful expression of a reporter gene in muscle[1], plasmid DNA injection has been widely used to mediate gene transfer study. As a gene transfer vector, naked DNA has many obvious advantages. It is easy and cheap to be prepared and more safe after transfection. But the plasmid mediated gene expression is low because of t…     

Shift visual cryptography scheme of two secret images

A new visual cryptography scheme of two secret images, which is named the shift visual cryptography of two secret images, is given. The compound function family of a general construction model is also introduced. The uniqueness and validity of this model are proved, and the minimum rows and columns for realizing the scheme are obtained. Compared with visual cryptography of one secret image, the shift visual cryptography scheme can share two secret images with the same size of pixels expansion without losing the contrast. It has randomness and various forms, and furthermore, we can also visually recover two secret images even if the two shares are different in form and color.     

Rapid cold hardening process of insects and its ecologically adaptive significance

In contrast to overwintering cold hardening, a rapid cold hardening process has recently been described in insects. This process can rapidly enhance cold tolerance of insects in several hours or even minutes. The characteristics of rapid cold hardening include inducing conditions, cold hardening effects, and fitness costs. Physiological and biochemical mechanisms of rapid cold hardening include rapid changes in the phospholipid composition and the increase in hemolymph osmolalities, polyols production, proline and cold-induced proteins. Moreover, there are different views about the ecologically adaptive significance of rapid cold hardening in insects. This paper reviews research results about rapid cold hardening of insects since 1987, focusing on forms of cold injury, strategies of cold tolerance, and definitions, characteristics, physiological and biochemical mechanisms and adaptive significance of rapid cold hardening.     

井下掺液抽稠油泵研究

通过对常规泵进行串联、改造 ,研制出了井下掺液抽稠油泵。对泵结构的可行性进行了论证 ,阐述了泵的工作原理 ,并对泵的工作参数进行了分析。该泵是通过从油管掺入液、从油套环空产出液 ,在上冲程时吸入产出液 ,在下冲程时举升混合液 ,从而实现了在柱塞下方掺液 ,避免了出现砂磨柱塞的现象 ,减少了稠油对柱塞运动的阻力 ,克服了泵上掺液不能降低进泵原油粘度的缺点 ,解决了在井筒降粘和稠油抽汲中存在的问题。该泵使用了连动式凡尔与弹簧 ,保证了底阀与顶阀打开与关闭的同步性 ,能够达到吸入产出液、加入掺入液以及举升混合液同时进行的目的。该泵的设计原理可行 ,工艺容易实现 ,是一种理想的新型抽稠油泵。     

湛江港海域台风浪分布特征模拟

利用物理过程考虑得较全面的第三代近岸海浪数值模式(SWAN)，模拟了湛江港海域一次台风浪过程。结果表明：台风风场直接决定了海浪的分布特征。在台风场的驱动下，风向与浪向基本上是一致的，并且波高、波长与周期的发展和传播方向与风向也是一致的；风速的大小决定了波高、波长与周期的大小。此外，海浪的分布特征受台风中心的位置和移动路径影响较大，当台风中心位于湛江港海域的不同位置时，台风浪有效波高、波长与周期的分布特征及传播方向都有着较大的差异，可以为湛江港海域台风浪的预报提供借鉴。     

Building of hFⅨ transgenic mice by spermatogenic cells

Human FⅨ expression vector pCMVⅨ was packaged by effectene^TM reagent and injected into mice seminiferous tubules with glass pipettes.The expressional frame of pCMVⅨ was examined by PCR and Southern blotting among 41 progenies.There were 2(4%) mice being integrated with hFⅨ gene into chromosomes.4.6ng/mL of hFⅨ protein was expressed in plasma of one mouse,which was tested by ELISA.We demonstrated that building of transgenic animals by spermatogonial stem cells is an efficient method.Meanwhile,it has also been proved to be an alternative choice for mammary gland bioreactor.     

微波诱导合成5-芳氧亚甲基-2-[5′-(4″-氯代苯基)- 2′-呋喃甲酰胺基]-1,3,4-噁二唑

在微波辐射条件下,由1 芳氧乙酰基 4 [5′ (4″ 氯代苯基) 2′ 呋喃甲酰基]氨基硫脲(1a～1i)在醋酸汞存在下,快速环合合成了一系列5 芳氧亚甲基 2 [5′ (4″ 氯代苯基) 2′ 呋喃甲酰胺基] 1,3,4 二唑(2a～2i).     

干燥控制化学添加剂在制备硅气凝胶中的应用

以硅溶胶为主要原料,通过硅溶胶体系的凝胶过程中添加干燥控制化学添加剂(DCCA),并结合非超临界干燥技术制备了硅气凝胶,研究了DCCA对凝胶过程和最终所得气凝胶品质的影响。DCCA能使凝胶的生成时间增加,凝胶结构均匀化,构成凝胶的基本微粒的粒径有所减小。在一定的DCCA添加量范围内,随着DCCA添加量的增加,所得气凝胶样品的密度有所减小,比表面积增加,微观结构变得更加完善,孔分布也更加集中。不同DCCA对气凝胶品质的影响规律不尽相同,气凝胶的品质就有可能通过调节添加DCCA的种类和量得到控制。     

演绎数据库虚关系的内部表示

介绍了演绎数据库的系统结构，并着重介绍了基于SQL Serverr之上的递归查询算法和将以规则形式存放于外存的虚关系转化为内部表示的过程。     

四乙基铵碳酸氢盐水主客体加合物的晶体结构研究

合成了(C2H5)4N+HCO-3*H2O加合物并用X射线单晶衍射解析了其晶体结构.晶体属单斜晶系, P21/n空间群, a=0.8186(1) nm, b=1.5330(3) nm, c=0.9562(3)nm, β=97.82(2)°, Z=4, V=1.1888(5) nm3, Mr=209.28, ρc=1.169g*cm-3, μ(MοKα)=0.090mm-1, F(000)=464,对1837个独立衍射点的最终偏离因子R=0.0486.每个不对称单位由1个碳酸氢根离子、1个水分子与1个四乙基铵阳离子组成.阴离子和水分子之间通过氢键连成皱折纽带,被包含在四乙基铵阳离子列所形成的未完全封闭管道中.