Interaction between poly（amidoamine） dendrimers and DNA studied by spectroscopic methods

The interaction between amino-terminated, and ethylenedtamine core poly（amtdoamine） （PAMAM） dendrimers and herring sperm DNA was investigated by various spectroscopic methods including UV spectroscopy, fluorescence spectroscopy, microscopic FTIR- and circular dichroism （CD-） spectroscopy. Ethidium bromide （EB） is used as a nucleic acid probe for this study. Experimental results show that PAMAM dendrimers can form stable complexes with DNA and the dendrimers bind to DNA sufficiently strong which cannot be displaced by EB, and we also found that the formation of the complexes can cause the conformation change of the DNA secondary structure. According to the Scatchard analysis, the association constant of PAMAM to DNA is calculated to be 2.53×10^4 mol/L^-1.     

Fabrication of PEDOT/PSS-ZnO nanowire by self-assembly method under vacuum condition

Poly(3,4-ethylenedioxythiophene) (PEDOT) has at- tracted much attention due to its high conductivity, excel- lent environmental stability and chemical processabil- ity[1,2]. PEDOT/PSS dispersion is a water-soluble system using poly(styrene sulfonic acid) (PSS) as the doping counterion during polymerization. The inclusion of PSS results in better solubility and processability to the PE- DOT/PSS aqueous dispersion[3, 4]. Recently, synthesis of one-dimensional (1D) multifunc- tional co…     

欠发达地区高校师生公正观念的表达与高校稳定

纵观历史,影响高校尤其是欠发达地区高校稳定的原因很多,但其中一个重要原因就是高校中存在的不公问题。本文分析了欠发达地区高校师生对不公正待遇的表达方式,研究了在公正问题上影响欠发达地区高校稳定的几个主要因素,并对消除社会不公、做好欠发达地区高校的稳定工作提出了相应的对策。     

Study on molecular interaction between NifA and NifL of Pseudomonas stutzeri A1501 by using the yeast two-hybrid system

Pseudomonas stutzeri AI501 (formerly Alcaligenes faecalis A1501), which was isolated from rice paddies in South China in 1980, can colonize tightly on rhizoplane of the host plants or invade the roots of plants for growth and nitrogen fixation. But A1501 can fix nitrogen only under the micro-aerobic and nitrogen-free conditions. The oxygen concentration and the availability of fixed nitrogen are therefore important factors in the regulation of nitrogenase biosynthesis of associative nitrogen-fixing bacteria.     

Microporous polymer electrolyte based on PVDF-PEO

Since Wright et al. found that the complex of PEO/alkali metals salt had the ability of ionic conductivity in 1973, in-depth studies have been carried out about various polymer electrolytes, which were applied to replacing the liquid electrolytes in lithium ion battery. At present, polymer electrolytes mainly include three kinds:     

Functional identification of gene cluster for the aniline metabolic pathway mediated by transposable element

A convenient and widely applicable method has been developed to clone aniline metabolic gene cluster in this study. Three positive recombinant plasmids pDA1, pDB2 and pDB11 were cloned from genomic library of aniline degradation strain AD9. The result of aniline dioxygenase （AD） activity and catechol 2,3-oxygenase （C230） activity assay showed that pDA1 and pDB11 contain aniline dioxygenase genes and catechol 2,3-dioxygenase genes, respectively. The sequence analysis of the total 24.7-kb region revealed that this region contains 25 ORFs, of which 17 genes involve metabolism of aniline. In the gene cluster, the first five genes （tadQTA1A2B） and the subsequent gene （tadR1） were predicted to encode a multi-component aniline dioxygenase and a LysR-type regulator, respectively, while the others （tadD1C1D2C2EFGIJKL） were expected to encode metacleavage pathway enzymes for catechol degradation. The gene cluster was surrounded by two IS1071 sequences.     

农业专家系统开发平台测试方案研究

主要阐述了对农业专家系统开发平台测试方案的研究及一些测试技术在其中的应用，提出了针对这类开发平台的一种分阶段、分步骤、分领域的测试方案，并运用案例分析了黑盒测试、应用操作覆盖测试和Web应用软件测试技术在测评这类开发平台中的应用。     

N-（2-氯苯甲酰基）-N′-[5-（3-吡啶基）-1，3，4-噻二唑-2-基]-硫脲的相转移催化法合成

在PEG-600为催化剂的固液相转移催化条件下用2-氯苯甲酰氯与硫氰酸铵及胺反应，一锅法制得N-（2-氯苯甲酰基）-N′-[5-（3-吡啶基）-1，3，4-噻二唑-2-基]-硫脲Ⅲ，其结构经元素分析、^1H NMR、IR和MS证实。     

一类二维离散混沌系统在不同系统参数下的自适应混沌同步

基于参量自适应控制中的混沌同步．针对一类二维离散混沌系统，提出了混沌系统在不同系统参数下的混沌同步方法，并给出了理论证明和实际应用例子。其理论结果很容易扩展到高维系统。     

基于IEEE802．11e MAC的联合无线资源管理

联合无线资源管理（JRRM）是针对耦舍多种无线接入技术的异构网络进行无线资源管理的一种新型机制，其目的是基于重配置技术和业务分流来整体优化异构网络的资源利用率。这种资源管理机制对其管理的各个接入网提出了QoS保证机制的要求。IEEE 802．11e的提出为WLAN提供了MAC层的QoS保证机制，从而促使IRRM能够高效工作。对基于IEEE 802．11e MAC的JRRM进行介绍，并分析了在联合会话允许控制和联合资源调度方面，基于IEEE802．11e MAC的方式能够改善异构无线环境中的网络性能。