A component of innate immunity prevents bacterial biofilm development

Antimicrobial factors form one arm of the innate immune system, which protects mucosal surfaces from bacterial infection. These factors can rapidly kill bacteria deposited on mucosal surfaces and prevent acute invasive infections. In many chronic infections, however, bacteria live in biofilms, which are distinct, matrix-encased communities specialized for surface persistence. The transition from a free-living, independent existence to a biofilm lifestyle can be devastating, because biofilms notoriously resist killing by host defence mechanisms and antibiotics. We hypothesized that the innate immune system possesses specific activity to protect against biofilm infections. Here we show that lactoferrin, a ubiquitous and abundant constituent of human external secretions, blocks biofilm development by the opportunistic pathogen Pseudomonas aeruginosa. This occurs at lactoferrin concentrations below those that kill or prevent growth. By chelating iron, lactoferrin stimulates twitching, a specialized form of surface motility, causing the bacteria to wander across the surface instead of forming cell clusters and biofilms. These findings reveal a specific anti-biofilm defence mechanism acting at a critical juncture in biofilm development, the time bacteria stop roaming as individuals and aggregate into durable communities.     

Genome analysis of the platypus reveals unique signatures of evolution

We present a draft genome sequence of the platypus, Ornithorhynchus anatinus. This monotreme exhibits a fascinating combination of reptilian and mammalian characters. For example, platypuses have a coat of fur adapted to an aquatic lifestyle; platypus females lactate, yet lay eggs; and males are equipped with venom similar to that of reptiles. Analysis of the first monotreme genome aligned these features with genetic innovations. We find that reptile and platypus venom proteins have been co-opted independently from the same gene families; milk protein genes are conserved despite platypuses laying eggs; and immune gene family expansions are directly related to platypus biology. Expansions of protein, non-protein-coding RNA and microRNA families, as well as repeat elements, are identified. Sequencing of this genome now provides a valuable resource for deep mammalian comparative analyses, as well as for monotreme biology and conservation.     

Global hotspots of species richness are not congruent with endemism or threat

Biodiversity hotspots have a prominent role in conservation biology, but it remains controversial to what extent different types of hotspot are congruent. Previous studies were unable to provide a general answer because they used a single biodiversity index, were geographically restricted, compared areas of unequal size or did not quantitatively compare hotspot types. Here we use a new global database on the breeding distribution of all known extant bird species to test for congruence across three types of hotspot. We demonstrate that hotspots of species richness, threat and endemism do not show the same geographical distribution. Only 2.5% of hotspot areas are common to all three aspects of diversity, with over 80% of hotspots being idiosyncratic. More generally, there is a surprisingly low overall congruence of biodiversity indices, with any one index explaining less than 24% of variation in the other indices. These results suggest that, even within a single taxonomic class, different mechanisms are responsible for the origin and maintenance of different aspects of diversity. Consequently, the different types of hotspots also vary greatly in their utility as conservation tools.     

Rapid assessment of acid phosphatase activity in the mycorrhizosphere and in arbuscular mycorrhizal fungal hyphae

A pot experiment has been carried out under controlled conditions to study the possibility of applying the technique ofin vivo staining for acid phosphatase activity on the roots of mycorrhizal plants and arbuscular mycorrhizal hyphae. The pots had 5 compartments. The central root compartment was separated from the two adjacent hyphal compartments using nylon nets of 30 μm mesh, and the two hyphal compartments were separated from the two outermost compartments with 0.45 μm membranes. Red clover was grown in the root compartment and was either inoculated with the arbuscular mycorrhizal fungus (AMF)Glomus mosseae or unlnoculated. Sodium phytate was applied to all compartments. The results show that AMF can increase acid phosphatase activity of clover roots. The plant roots acquired deep red “mycorrhizal prints”. The external hyphae also had obvious “hyphal prints” on the test papers, indicating the ability of mycorrhizal hyphae to release acid phosphatase.     

Timescale classification in wind forecasting: A review of the state-of-the-art

The intermittency of the wind has been reported to present significant challenges to power and grid systems, which intensifies with increasing penetration levels. Accurate wind forecasting can mitigate these challenges and help in integrating more wind power into the grid. A range of studies have presented algorithms to forecast the wind in terms of wind speeds and wind power generation across different timescales. However, the classification of timescales varies significantly across the different studies (2010–2014). The timescale is important in specifying which methodology to use when, as well in uniting future research, data requirements, etc. This study proposes a generic statement on how to classify the timescales, and further presents different applications of these forecasts across the entire wind power value chain.     

Chromosomal clustering of muscle-expressed genes in Caenorhabditis elegans

Chromosomes are divided into domains of open chromatin, where genes have the potential to be expressed, and domains of closed chromatin, where genes are not expressed. Classic examples of open chromatin domains include 'puffs' on polytene chromosomes in Drosophila and extended loops from lampbrush chromosomes. If multiple genes were typically expressed together from a single open chromatin domain, the position of co-expressed genes along the chromosomes would appear clustered. To investigate whether co-expressed genes are clustered, we examined the chromosomal positions of the genes expressed in the muscle of Caenorhabditis elegans at the first larval stage. Here we show that co-expressed genes in C. elegans are clustered in groups of 2-5 along the chromosomes, suggesting that expression from a chromatin domain can extend over several genes. These observations reveal a higher-order organization of the structure of the genome, in which the order of the genes along the chromosome id correlated with their expression in specific tissues.     

Neurodegenerative disease: amyloid pores from pathogenic mutations

Alzheimer's and Parkinson's diseases are associated with the formation in the brain of amyloid fibrils from beta-amyloid and alpha-synuclein proteins, respectively. It is likely that oligomeric fibrillization intermediates (protofibrils), rather than the fibrils themselves, are pathogenic, but the mechanism by which they cause neuronal death remains a mystery. We show here that mutant amyloid proteins associated with familial Alzheimer's and Parkinson's diseases form morphologically indistinguishable annular protofibrils that resemble a class of pore-forming bacterial toxins, suggesting that inappropriate membrane permeabilization might be the cause of cell dysfunction and even cell death in amyloid diseases.     

Origin of a 'Southern Hemisphere' geochemical signature in the Arctic upper mantle

The Gakkel ridge, which extends under the Arctic ice cap for approximately 1,800 km, is the slowest spreading ocean ridge on Earth. Its spreading created the Eurasian basin, which is isolated from the rest of the oceanic mantle by North America, Eurasia and the Lomonosov ridge. The Gakkel ridge thus provides unique opportunities to investigate the composition of the sub-Arctic mantle and mantle heterogeneity and melting at the lower limits of seafloor spreading. The first results of the 2001 Arctic Mid-Ocean Ridge Expedition (ref. 1) divided the Gakkel ridge into three tectonic segments, composed of robust western and eastern volcanic zones separated by a 'sparsely magmatic zone'. On the basis of Sr-Nd-Pb isotope ratios and trace elements in basalts from the spreading axis, we show that the sparsely magmatic zone contains an abrupt mantle compositional boundary. Basalts to the west of the boundary display affinities to the Southern Hemisphere 'Dupal' isotopic province, whereas those to the east-closest to the Eurasian continent and where the spreading rate is slowest-display affinities to 'Northern Hemisphere' ridges. The western zone is the only known spreading ridge outside the Southern Hemisphere that samples a significant upper-mantle region with Dupal-like characteristics. Although the cause of Dupal mantle has been long debated, we show that the source of this signature beneath the western Gakkel ridge was subcontinental lithospheric mantle that delaminated and became integrated into the convecting Arctic asthenosphere. This occurred as North Atlantic mantle propagated north into the Arctic during the separation of Svalbard and Greenland.