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Bitoun M Maugenre S Jeannet PY Lacène E Ferrer X Laforêt P Martin JJ Laporte J Lochmüller H Beggs AH Fardeau M Eymard B Romero NB Guicheney P 《Nature genetics》2005,37(11):1207-1209
Autosomal dominant centronuclear myopathy is a rare congenital myopathy characterized by delayed motor milestones and muscular weakness. In 11 families affected by centronuclear myopathy, we identified recurrent and de novo missense mutations in the gene dynamin 2 (DNM2, 19p13.2), which encodes a protein involved in endocytosis and membrane trafficking, actin assembly and centrosome cohesion. The transfected mutants showed reduced labeling in the centrosome, suggesting that DNM2 mutations might cause centronuclear myopathy by interfering with centrosome function. 相似文献
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Hattori M Fujiyama A Taylor TD Watanabe H Yada T Park HS Toyoda A Ishii K Totoki Y Choi DK Groner Y Soeda E Ohki M Takagi T Sakaki Y Taudien S Blechschmidt K Polley A Menzel U Delabar J Kumpf K Lehmann R Patterson D Reichwald K Rump A Schillhabel M Schudy A Zimmermann W Rosenthal A Kudoh J Schibuya K Kawasaki K Asakawa S Shintani A Sasaki T Nagamine K Mitsuyama S Antonarakis SE Minoshima S Shimizu N Nordsiek G Hornischer K Brant P Scharfe M Schon O Desario A Reichelt J Kauer G Blocker H 《Nature》2000,405(6784):311-319
Chromosome 21 is the smallest human autosome. An extra copy of chromosome 21 causes Down syndrome, the most frequent genetic cause of significant mental retardation, which affects up to 1 in 700 live births. Several anonymous loci for monogenic disorders and predispositions for common complex disorders have also been mapped to this chromosome, and loss of heterozygosity has been observed in regions associated with solid tumours. Here we report the sequence and gene catalogue of the long arm of chromosome 21. We have sequenced 33,546,361 base pairs (bp) of DNA with very high accuracy, the largest contig being 25,491,867 bp. Only three small clone gaps and seven sequencing gaps remain, comprising about 100 kilobases. Thus, we achieved 99.7% coverage of 21q. We also sequenced 281,116 bp from the short arm. The structural features identified include duplications that are probably involved in chromosomal abnormalities and repeat structures in the telomeric and pericentromeric regions. Analysis of the chromosome revealed 127 known genes, 98 predicted genes and 59 pseudogenes. 相似文献
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M. Zimmermann 《Cellular and molecular life sciences : CMLS》1954,10(3):145-146
Summary The phloem sap ofRobinia Pseud-Acacia contains only sucrose and does not possess invertase activity. A sucrase is, however, present in nectar collected fromRobinia flowers: this enzyme identified as a transglucosidase, liberates greater amounts of fructose than of glucose from sucrose. Enzymic activity apparently commences 2–5 days before the flowers are fully open.
Die vorliegenden Untersuchungen erfolgten im Rahmen der Arbeitsgemeinschaft Prof. Dr.A. Frey-Wyssling, Zürich, und Dr.A. Maurizio, Bern, mit Unterstützung der Fritz-Hoffmann-La-Roche-Stiftung zur Förderung wissenschaftlicher Arbeitsgemeinschaften in der Schweiz. 相似文献
Die vorliegenden Untersuchungen erfolgten im Rahmen der Arbeitsgemeinschaft Prof. Dr.A. Frey-Wyssling, Zürich, und Dr.A. Maurizio, Bern, mit Unterstützung der Fritz-Hoffmann-La-Roche-Stiftung zur Förderung wissenschaftlicher Arbeitsgemeinschaften in der Schweiz. 相似文献
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Timm Schubert Corinna Gleiser Peter Heiduschka Christoph Franz Kerstin Nagel-Wolfrum Ayse Sahaboglu Nicole Weisschuh Gordon Eske Karin Rohbock Norman Rieger François Paquet-Durand Bernd Wissinger Uwe Wolfrum Bernhard Hirt Wibke Singer Lukas Rüttiger Ulrike Zimmermann Marlies Knipper 《Cellular and molecular life sciences : CMLS》2015,72(20):3953-3969
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Gram-negative bacteria, such as Escherichia coli, expel toxic chemicals through tripartite efflux pumps that span both the inner and outer membrane. The three parts are an inner membrane, substrate-binding transporter; a membrane fusion protein; and an outer-membrane-anchored channel. The fusion protein connects the transporter to the channel within the periplasmic space. A crystallographic model of this tripartite efflux complex has been unavailable because co-crystallization of the various components of the system has proven to be extremely difficult. We previously described the crystal structures of both the inner membrane transporter CusA and the membrane fusion protein CusB of the CusCBA efflux system of E. coli. Here we report the co-crystal structure of the CusBA efflux complex, showing that the transporter (or pump) CusA, which is present as a trimer, interacts with six CusB protomers and that the periplasmic domain of CusA is involved in these interactions. The six CusB molecules seem to form a continuous channel. The affinity of the CusA and CusB interaction was found to be in the micromolar range. Finally, we have predicted a three-dimensional structure for the trimeric CusC outer membrane channel and developed a model of the tripartite efflux assemblage. This CusC(3)-CusB(6)-CusA(3) model shows a 750-kilodalton efflux complex that spans the entire bacterial cell envelope and exports Cu I and Ag I ions. 相似文献
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Monique De Mendonca Pascale Guicheney Marie-Laure Grichois D. Ben-Ishay P. Meyer 《Cellular and molecular life sciences : CMLS》1981,37(10):1087-1089
Summary Plasma catecholamines are increased in sodium-loaded rats under both resting and stress conditions. Under stress, Na+ resistant rats have lower plasma catecholamines than salt-sensitive ones.Supported in part by grants from Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de la Recherche Scientifique (CNRS) and Délégation Générale à la Recherche Scientifique et Technique (DGRST). 相似文献