基于动能密度时间变化率的固/液流动分离判据

在空间场中动能密度的时间变化率有许多极小值,定义流动分离发生在其最小值处．可利用流体的无粘流动解计算动能密度值,而采用保角变换的方法,则可获得复杂几何形状下的有势流场解,从而很容易确定出可能的流动分离位置．将此判据应用于流线外型的设计,对延迟流动分离及减少阻力是有帮助的．对于均匀来流的圆柱绕流问题,该判据预示流动分离发生在离后驻点角度为±５４．７４°处,而已知的实验数据指出：流动分离发生在±５０°与±５８°之间．对于长轴∶短轴＝１∶６的椭圆翼剖面,当来流攻角在７°与８°之间时,将开始发生严重的失速现象．英国皇家空军３４翼剖面具有相同的长短轴比）的实验数据表明,失速开始发生在１２°与１４°攻角之间,可见理论值与实验值接近．文中讨论了其它形状的翼剖面,说明如何通过选择机翼的形状来延迟失速现象的发生．     

Metabolic network analysis of the causes and evolution of enzyme dispensability in yeast

Under laboratory conditions 80% of yeast genes seem not to be essential for viability. This raises the question of what the mechanistic basis for dispensability is, and whether it is the result of selection for buffering or an incidental side product. Here we analyse these issues using an in silico flux model of the yeast metabolic network. The model correctly predicts the knockout fitness effects in 88% of the genes studied and in vivo fluxes. Dispensable genes might be important, but under conditions not yet examined in the laboratory. Our model indicates that this is the dominant explanation for apparent dispensability, accounting for 37-68% of dispensable genes, whereas 15-28% of them are compensated by a duplicate, and only 4-17% are buffered by metabolic network flux reorganization. For over one-half of those not important under nutrient-rich conditions, we can predict conditions when they will be important. As expected, such condition-specific genes have a more restricted phylogenetic distribution. Gene duplicates catalysing the same reaction are not more common for indispensable reactions, suggesting that the reason for their retention is not to provide compensation. Instead their presence is better explained by selection for high enzymatic flux.     

Patterns of dye coupling in the imaginal wing disk of Drosophila melanogaster

Responses of developing tissues to experimental disruption demonstrate that cell interaction is important both in generating positional information and in controlling growth. However, the mechanism by which cells interact and the range over which the interactions are effective are not known. In the imaginal disks of Drosophila melanogaster, experiments on pattern regulation following surgical ablation suggest that the cell interactions are very local in nature; in fact, most of the data can be explained by assuming that cells interact only with their immediate neighbours. In contrast, studies of cell division patterns in the same tissue indicate that the "local' proliferative response to an ablation extends over a distance of up to about eight cell diameters. Still longer-range interactions have been proposed on the basis of theoretical considerations. It is possible that the interactions are mediated by the transfer of small molecules through gap junctions, as gap junctions are abundant in imaginal disks at the appropriate developmental stages. We have explored the range, timing and directionality of dye coupling between the cells of the wing disk as a test of the possible role of gap junctions in imaginal disk patterning. Our results indicate that interactions over different ranges are possible depending on the nature of the molecule being transferred.     

Monoclonal antibody identifies a new Ia-like (p29,34) polymorphic system linked to the HLA-D/DR region

The Ia antigens of the mouse are the basis for the genetic control of the immune response. The HLA-D/DR locus is considered to be the human counterpart of the Ia subregion of the murine major histocompatibility complex. The HLA-D/DR antigens are polymorphic, and eight well defined alleles have been identified using alloantisera. More recently, 'supertypic' antigens (MB and MT) have been defined which identify clusters of HLA-D/DR specificities. Little is known about the molecular basis for the cellular and serological polymorphism of the HLA-D/DR antigens, as alloantisera are usually of very low titre and heteroantisera frequently lack monospecificity. We present here the preparation and characterization of a monoclonal antibody which defines a new polymorphic system of the HLA-D/DR region. This and similar antisera should now begin to provide the reagents with which to correlate molecular structure with the functional repertoire of the human Ia-like antigens.     

Sequence-directed curvature of DNA

DNAs from both prokaryotic and eukaryotic organisms have yielded restriction fragments which manifest markedly anomalous electrophoretic behaviour (reduced mobility) when run on polyacrylamide gels. We have shown previously that the abnormal electrophoretic behaviour of one such fragment is a consequence of stable curvature of the helix axis in solution. The molecules involved tend to contain oligo(dA)-oligo(dT) runs which are approximately in-phase with the helix repeat; however, the precise structural elements responsible for DNA curvature have not been identified. One popular model for curvature invokes a non-coplanar 'wedge-like' conformation of ApA/TpT dinucleotide pairs. Despite a lack of direct evidence in support of this model, it has been used to provide quantitative estimates of curvature. To critically evaluate the ApA wedge model, we have performed an electrophoretic analysis of a series of closely related DNA polymers in which oligo(dA)-oligo(dT) runs of different polarity were compared. We conclude that ApA dinucleotide wedges cannot account for DNA curvature. Therefore, quantitative estimates for ApA wedge deformations, based solely on apparent curvature, cannot be correct.     

The role of microbes in accretion, lamination and early lithification of modern marine stromatolites

For three billion years, before the Cambrian diversification of life, laminated carbonate build-ups called stromatolites were widespread in shallow marine seas. These ancient structures are generally thought to be microbial in origin and potentially preserve evidence of the Earth's earliest biosphere. Despite their evolutionary significance, little is known about stromatolite formation, especially the relative roles of microbial and environmental factors in stromatolite accretion. Here we show that growth of modern marine stromatolites represents a dynamic balance between sedimentation and intermittent lithification of cyanobacterial mats. Periods of rapid sediment accretion, during which stromatolite surfaces are dominated by pioneer communities of gliding filamentous cyanobacteria, alternate with hiatal intervals. These discontinuities in sedimentation are characterized by development of surface films of exopolymer and subsequent heterotrophic bacterial decomposition, forming thin crusts of microcrystalline carbonate. During prolonged hiatal periods, climax communities develop, which include endolithic coccoid cyanobacteria. These coccoids modify the sediment, forming thicker lithified laminae. Preservation of lithified layers at depth creates millimetre-scale lamination. This simple model of modern marine stromatolite growth may be applicable to ancient stromatolites.     

Attribution of observed surface humidity changes to human influence

Water vapour is the most important contributor to the natural greenhouse effect, and the amount of water vapour in the atmosphere is expected to increase under conditions of greenhouse-gas-induced warming, leading to a significant feedback on anthropogenic climate change. Theoretical and modelling studies predict that relative humidity will remain approximately constant at the global scale as the climate warms, leading to an increase in specific humidity. Although significant increases in surface specific humidity have been identified in several regions, and on the global scale in non-homogenized data, it has not been shown whether these changes are due to natural or human influences on climate. Here we use a new quality-controlled and homogenized gridded observational data set of surface humidity, with output from a coupled climate model, to identify and explore the causes of changes in surface specific humidity over the late twentieth century. We identify a significant global-scale increase in surface specific humidity that is attributable mainly to human influence. Specific humidity is found to have increased in response to rising temperatures, with relative humidity remaining approximately constant. These changes may have important implications, because atmospheric humidity is a key variable in determining the geographical distribution and maximum intensity of precipitation, the potential maximum intensity of tropical cyclones, and human heat stress, and has important effects on the biosphere and surface hydrology.     

基于TCP/IP的局域安保监视系统

实现一个由ZR36060完成图像编解码，CS8900芯片实现TCP／IP协议，完成数据传榆，单片机则完成数据的调度、管理，对各部件的初始化和控制，完全不依赖复杂的实时操作系统，仅依靠单片机自身的监控系统和部分自主开发的应用软件，即可实现一个瘦客户型的局域安保监视系统。