全文获取类型
收费全文 | 6015篇 |
免费 | 30篇 |
国内免费 | 16篇 |
专业分类
系统科学 | 119篇 |
丛书文集 | 24篇 |
教育与普及 | 6篇 |
理论与方法论 | 61篇 |
现状及发展 | 3239篇 |
研究方法 | 344篇 |
综合类 | 2171篇 |
自然研究 | 97篇 |
出版年
2018年 | 58篇 |
2017年 | 50篇 |
2016年 | 62篇 |
2015年 | 42篇 |
2014年 | 49篇 |
2013年 | 50篇 |
2012年 | 153篇 |
2011年 | 234篇 |
2010年 | 80篇 |
2008年 | 154篇 |
2007年 | 140篇 |
2006年 | 153篇 |
2005年 | 155篇 |
2004年 | 174篇 |
2003年 | 131篇 |
2002年 | 125篇 |
2001年 | 145篇 |
2000年 | 130篇 |
1999年 | 98篇 |
1992年 | 103篇 |
1991年 | 67篇 |
1990年 | 71篇 |
1989年 | 53篇 |
1987年 | 70篇 |
1986年 | 63篇 |
1985年 | 76篇 |
1984年 | 84篇 |
1983年 | 42篇 |
1982年 | 62篇 |
1981年 | 43篇 |
1980年 | 76篇 |
1979年 | 178篇 |
1978年 | 129篇 |
1977年 | 171篇 |
1976年 | 73篇 |
1975年 | 90篇 |
1974年 | 192篇 |
1973年 | 162篇 |
1972年 | 175篇 |
1971年 | 153篇 |
1970年 | 200篇 |
1969年 | 195篇 |
1968年 | 179篇 |
1967年 | 187篇 |
1966年 | 178篇 |
1965年 | 129篇 |
1964年 | 46篇 |
1958年 | 58篇 |
1957年 | 46篇 |
1956年 | 44篇 |
排序方式: 共有6061条查询结果,搜索用时 15 毫秒
951.
Regular meteor showers occur when a planet approaches the orbit of a periodic comet--for example, the Leonid shower is evident around 17 November every year as Earth skims past the dusty trail of comet Tempel-Tuttle. Such showers are expected to occur on Mars as well, and on 7 March last year, the panoramic camera of Spirit, the Mars Exploration Rover, revealed a curious streak across the martian sky. Here we show that the timing and orientation of this streak, and the shape of its light curve, are consistent with the existence of a regular meteor shower associated with the comet Wiseman-Skiff, which could be characterized as martian Cepheids. 相似文献
952.
Suppression of Polycomb group proteins by JNK signalling induces transdetermination in Drosophila imaginal discs 总被引:1,自引:0,他引:1
During the regeneration of Drosophila imaginal discs, cellular identities can switch fate in a process known as transdetermination. For leg-to-wing transdetermination, the underlying mechanism involves morphogens such as Wingless that, when activated outside their normal context, induce ectopic expression of the wing-specific selector gene vestigial. Polycomb group (PcG) proteins maintain cellular fates by controlling the expression patterns of homeotic genes and other developmental regulators. Here we report that transdetermination events are coupled to PcG regulation. We show that the frequency of transdetermination is enhanced in PcG mutant flies. Downregulation of PcG function, as monitored by the reactivation of a silent PcG-regulated reporter gene, is observed in transdetermined cells. This downregulation is directly controlled by the Jun amino-terminal kinase (JNK) signalling pathway, which is activated in cells undergoing regeneration. Accordingly, transdetermination frequency is reduced in a JNK mutant background. This regulatory interaction also occurs in mammalian cells, indicating that the role of this signalling cascade in remodelling cellular fates may be conserved. 相似文献
953.
Eukaryotic members of the CLC gene family function as plasma membrane chloride channels, or may provide neutralizing anion currents for V-type H(+)-ATPases that acidify compartments of the endosomal/lysosomal pathway. Loss-of-function mutations in the endosomal protein ClC-5 impair renal endocytosis and lead to kidney stones, whereas loss of function of the endosomal/lysosomal protein ClC-7 entails osteopetrosis and lysosomal storage disease. Vesicular CLCs have been thought to be Cl- channels, in particular because ClC-4 and ClC-5 mediate plasma membrane Cl- currents upon heterologous expression. Here we show that these two mainly endosomal CLC proteins instead function as electrogenic Cl-/H+ exchangers (also called antiporters), resembling the transport activity of the bacterial protein ClC-e1, the crystal structure of which has already been determined. Neutralization of a critical glutamate residue not only abolished the steep voltage-dependence of transport, but also eliminated the coupling of anion flux to proton counter-transport. ClC-4 and ClC-5 may still compensate the charge accumulation by endosomal proton pumps, but are expected to couple directly vesicular pH gradients to Cl- gradients. 相似文献
954.
Ross AJ May-Simera H Eichers ER Kai M Hill J Jagger DJ Leitch CC Chapple JP Munro PM Fisher S Tan PL Phillips HM Leroux MR Henderson DJ Murdoch JN Copp AJ Eliot MM Lupski JR Kemp DT Dollfus H Tada M Katsanis N Forge A Beales PL 《Nature genetics》2005,37(10):1135-1140
The evolutionarily conserved planar cell polarity (PCP) pathway (or noncanonical Wnt pathway) drives several important cellular processes, including epithelial cell polarization, cell migration and mitotic spindle orientation. In vertebrates, PCP genes have a vital role in polarized convergent extension movements during gastrulation and neurulation. Here we show that mice with mutations in genes involved in Bardet-Biedl syndrome (BBS), a disorder associated with ciliary dysfunction, share phenotypes with PCP mutants including open eyelids, neural tube defects and disrupted cochlear stereociliary bundles. Furthermore, we identify genetic interactions between BBS genes and a PCP gene in both mouse (Ltap, also called Vangl2) and zebrafish (vangl2). In zebrafish, the augmented phenotype results from enhanced defective convergent extension movements. We also show that Vangl2 localizes to the basal body and axoneme of ciliated cells, a pattern reminiscent of that of the BBS proteins. These data suggest that cilia are intrinsically involved in PCP processes. 相似文献
955.
Poulet F Bibring JP Mustard JF Gendrin A Mangold N Langevin Y Arvidson RE Gondet B Gomez C Berthé M Erard S Forni O Manaud N Poulleau G Soufflot A Combes M Drossart P Encrenaz T Fouchet T Melchiorri R Bellucci G Altieri F Formisano V Fonti S Capaccioni F Cerroni P Coradini A Korablev O Kottsov V Ignatiev N Titov D Zasova L Pinet P Schmitt B Sotin C Hauber E Hoffmann H Jaumann R Keller U Forget F;Omega Team 《Nature》2005,438(7068):623-627
The recent identification of large deposits of sulphates by remote sensing and in situ observations has been considered evidence of the past presence of liquid water on Mars. Here we report the unambiguous detection of diverse phyllosilicates, a family of aqueous alteration products, on the basis of observations by the OMEGA imaging spectrometer on board the Mars Express spacecraft. These minerals are mainly associated with Noachian outcrops, which is consistent with an early active hydrological system, sustaining the long-term contact of igneous minerals with liquid water. We infer that the two main families of hydrated alteration products detected-phyllosilicates and sulphates--result from different formation processes. These occurred during two distinct climatic episodes: an early Noachian Mars, resulting in the formation of hydrated silicates, followed by a more acidic environment, in which sulphates formed. 相似文献
956.
Kinchen JM Cabello J Klingele D Wong K Feichtinger R Schnabel H Schnabel R Hengartner MO 《Nature》2005,434(7029):93-99
The removal of apoptotic cells is essential for the physiological well being of the organism. In Caenorhabditis elegans, two conserved, partially redundant genetic pathways regulate this process. In the first pathway, the proteins CED-2, CED-5 and CED-12 (mammalian homologues CrkII, Dock180 and ELMO, respectively) function to activate CED-10 (Rac1). In the second group, the candidate receptor CED-1 (CD91/LRP/SREC) probably recognizes an unknown ligand on the apoptotic cell and signals via its cytoplasmic tail to the adaptor protein CED-6 (hCED-6/GULP), whereas CED-7 (ABCA1) is thought to play a role in membrane dynamics. Molecular understanding of how the second pathway promotes engulfment of the apoptotic cell is lacking. Here, we show that CED-1, CED-6 and CED-7 are required for actin reorganization around the apoptotic cell corpse, and that CED-1 and CED-6 colocalize with each other and with actin around the dead cell. Furthermore, we find that the CED-10(Rac) GTPase acts genetically downstream of these proteins to mediate corpse removal, functionally linking the two engulfment pathways and identifying the CED-1, -6 and -7 signalling module as upstream regulators of Rac activation. 相似文献
957.
The counter-intuitive properties of quantum mechanics have the potential to revolutionize information processing by enabling the development of efficient algorithms with no known classical counterparts. Harnessing this power requires the development of a set of building blocks, one of which is a method to initialize the set of quantum bits (qubits) to a known state. Additionally, fresh ancillary qubits must be available during the course of computation to achieve fault tolerance. In any physical system used to implement quantum computation, one must therefore be able to selectively and dynamically remove entropy from the part of the system that is to be mapped to qubits. One such method is an 'open-system' cooling protocol in which a subset of qubits can be brought into contact with an external system of large heat capacity. Theoretical efforts have led to an implementation-independent cooling procedure, namely heat-bath algorithmic cooling. These efforts have culminated with the proposal of an optimal algorithm, the partner-pairing algorithm, which was used to compute the physical limits of heat-bath algorithmic cooling. Here we report the experimental realization of multi-step cooling of a quantum system via heat-bath algorithmic cooling. The experiment was carried out using nuclear magnetic resonance of a solid-state ensemble three-qubit system. We demonstrate the repeated repolarization of a particular qubit to an effective spin-bath temperature, and alternating logical operations within the three-qubit subspace to ultimately cool a second qubit below this temperature. Demonstration of the control necessary for these operations represents an important step forward in the manipulation of solid-state nuclear magnetic resonance qubits. 相似文献
958.
Establishing the age at death of individuals is an important step in their identification and can be done with high precision up to adolescence by analysis of dentition, but it is more difficult in adults. Here we show that the amount of radiocarbon present in tooth enamel as a result of nuclear bomb testing during 1955-63 is a remarkably accurate indicator of when a person was born. Age is determined to within 1.6 years, whereas the commonly used morphological evaluation of skeletal remains and tooth wear is sensitive to within 5-10 years in adults. 相似文献
959.
A quasar is thought to be powered by the infall of matter onto a supermassive black hole at the centre of a massive galaxy. Because the optical luminosity of quasars exceeds that of their host galaxy, disentangling the two components can be difficult. This led in the 1990s to the controversial claim of the discovery of 'naked' quasars. Since then, the connection between quasars and galaxies has been well established. Here we report the discovery of a quasar lying at the edge of a gas cloud, whose size is comparable to that of a small galaxy, but whose spectrum shows no evidence for stars. The gas in the cloud is excited by the quasar itself. If a host galaxy is present, it is at least six times fainter than would normally be expected for such a bright quasar. The quasar is interacting dynamically with a neighbouring galaxy, whose gas might be feeding the black hole. 相似文献
960.
Gehrels N Sarazin CL O'Brien PT Zhang B Barbier L Barthelmy SD Blustin A Burrows DN Cannizzo J Cummings JR Goad M Holland ST Hurkett CP Kennea JA Levan A Markwardt CB Mason KO Meszaros P Page M Palmer DM Rol E Sakamoto T Willingale R Angelini L Beardmore A Boyd PT Breeveld A Campana S Chester MM Chincarini G Cominsky LR Cusumano G de Pasquale M Fenimore EE Giommi P Gronwall C Grupe D Hill JE Hinshaw D Hjorth J Hullinger D Hurley KC Klose S Kobayashi S Kouveliotou C Krimm HA Mangano V 《Nature》2005,437(7060):851-854
Gamma-ray bursts (GRBs) come in two classes: long (> 2 s), soft-spectrum bursts and short, hard events. Most progress has been made on understanding the long GRBs, which are typically observed at high redshift (z approximately 1) and found in subluminous star-forming host galaxies. They are likely to be produced in core-collapse explosions of massive stars. In contrast, no short GRB had been accurately (< 10') and rapidly (minutes) located. Here we report the detection of the X-ray afterglow from--and the localization of--the short burst GRB 050509B. Its position on the sky is near a luminous, non-star-forming elliptical galaxy at a redshift of 0.225, which is the location one would expect if the origin of this GRB is through the merger of neutron-star or black-hole binaries. The X-ray afterglow was weak and faded below the detection limit within a few hours; no optical afterglow was detected to stringent limits, explaining the past difficulty in localizing short GRBs. 相似文献