城市水循环健康的评价方法及其应用——以邯郸市主城区为例

健康良性的水循环是实现区域可持续发展的基本保障。为客观诊断邯郸市水循环健康状况,选用综合指数法对其主城区2005～2016年水循环进行了健康评价,并采用模糊模式识别法和集对分析法进行对比验证。结果表明：邯郸市主城区因受到重工业城市背景和产业结构不合理的影响,城市水循环状态为亚健康;随着邯郸市产业结构的调整和水利民生政策的落实,其水循环健康状况逐年好转,2016年已达健康状态。三种评价方法的结果趋势基本一致,说明评价指标体系的构建较为合理,评价方法也较为适用。可见,研究结果为区域水资源综合管理的进一步优化以及社会经济的高质量发展提供了技术参考。     

基于VMD-LSTM-IPSO-GRU的电力负荷预测

为了挖掘电力负荷数据中的潜藏信息,提高负荷预测的精度,针对电力负荷强非线性、非平稳性等特点,提出一种基于变分模态分解(variational mode decomposition,VMD)、优化长短期神经网络(long-term and short-term memory network,LSTM)、改进的粒子群算法(improve particle swarm optimization,IPSO)优化门控循环单元(gated recurrent unit neural network,GRU)的混合预测模型。首先,使用相关性分析确定确定输入因素,再将负荷数据运用VMD算法结合样本熵分解为一系列本征模态分量(intrinsic mode fuction,IMF)和残差量,更加合理地确定分解层数和惩罚因子;其次,根据过零率将这些量划分为低频和高频,低频分量使用LSTM网络,高频分量利用IPSO-GRU网络分别进行预测;最后,将预测结果重构得到电力负荷的最终结果。仿真结果表明,相对于其它常规模型,该混合模型可有效的提取模态特征,具有更高的预测精度。     

A relevant in vitro rat model for the evaluation of blood-brain barrier translocation of nanoparticles

Poly(MePEG₂₀₀₀cyanoacrylate-co-hexadecylcyanoacrylate) (PEG-PHDCA) nanoparticles have demonstrated their capacity to reach the rat central nervous system after intravenous injection. For insight into the transport of colloidal systems across the blood-brain barrier (BBB), we developed a relevant in vitro rat BBB model consisting of a coculture of rat brain endothelial cells (RBECs) and rat astrocytes. The RBECs used in our model displayed and retained structural characteristics of brain endothelial cells, such as expression of P-glycoprotein, occludin and ZO-1, and immunofluorescence studies showed the specific localization of occludin and ZO1. The high values of transendothelial electrical resistance and low permeability coefficients of marker molecules demonstrated the functionality of this model. The comparative passage of polyhexadecylcyanoacrylate and PEG-PHDCA nanoparticles through this model was investigated, showing a higher passage of PEGylated nanoparticles, presumably by endocytosis. This result was confirmed by confocal microscopy. Thanks to a good in vitro/in vivo correlation, this rat BBB model will help in understanding the mechanisms of nanoparticle translocation and in designing new types of colloidal carriers as brain delivery systems.Received 4 March 2005; accepted 14 April 2005     

Functional and physical communication between oncoproteins and tumor suppressors

The discovery of oncogenes (c-onc’s) and tumor suppressors (TS’s) has led to the concept that cancer arises from defects in each of these classes of genes or their products. More recently, it has been appreciated that c-onc and TS proteins often affect one another’s functions. Within this context, I review the two classical TS’s, p53 and the retinoblastoma protein, and the consequences of their inactivation. The various forms of genomic instability (GI) that underly the high mutation rates of transformed cells are then discussed. Particular emphasis is placed upon the concept that GI is not only an integral part of the transformed state but is a prerequisite. Increased oxidative DNA damage, and/or an inabiliy to repair it, can lead to GI. The review then discusses recent observations showing that loss of the TS protein peroxiredoxin 1 (prdx1) and increased expression of the c-onc protein c-Myc, each leads to increased oxidative DNA damage. The critical nature of the c-onc-TS interaction is underscored by that occurring between prdx1 and c-Myc, with the former protein regulating the production of DNA-damaging reactive oxygen species by the latter. The intimate association between these proteins and others serves as a paradigm for the exquisite balancing act that c-onc’s and TS’s must maintain in order to properly control normal DNA replication and cellular proliferation while simultaneously minimizing the acquisition of potentially neoplastic mutations. Received 10 May 2005; received after revision 3 July 2005; accepted 19 July 2005     

Mechanisms underlying celiac disease and its neurologic manifestations

The extra-intestinal manifestations of celiac disease (CD), including ataxia and peripheral neuropathy, are increasingly being recognized as the presenting symptoms of this autoimmune disease. Although there is a greater understanding of the pathogenesis of the intestinal lesions in CD the mechanisms behind the neurologic manifestations of CD have not been elucidated. In this article, the authors review the cellular and molecular mechanisms behind the histopathologic changes in the intestine, discuss the presentation and characteristics of neurologic manifestations of CD, review the data on the mechanisms behind these manifestations, and discuss the diagnosis and treatment of CD. Molecular mimicry and intermolecular help may play a role in the development of neurologic complications.Received 11 March 2004; received after revision 29 October 2004; accepted 12 November 2004     

Lactoferrin

Mammalian lactoferrin (Lf) receptors are suggested to have pivotal roles for mediating multiple functions of Lf. In this review, we focus on current knowledge of the structure and function of mammalian Lf receptors, mainly the first cloned Lf receptor that has been shown to be expressed in the infant small intestine at high levels but also in virtually all other tissues. The small intestinal Lf receptor takes up iron from Lf into cells and presumably exerts other physiological functions. Other Lf receptors in various tissues have also been reported to mediate some functions of Lf, such as modulating immune function, inhibiting platelet aggregation and enhancing collagen gel contractile strength. The detailed mechanisms behind the receptor-Lf interactions still need to be elucidated.     

Is chlamydial heat shock protein 60 a risk factor for oncogenesis?

Heat shock protein 60 (HSP60) plays an important role in the protein folding of prokaryotic and eukaryotic cells. Most of the papers published on chlamydial HSP60 concern its role in immune response during infection. In the last decade, exposure to Chlamydia trachomatis has been consistently associated with the development of cervical and ovarian cancer. Moreover, it has been suggested that chlamydial HSP60 may have an anti-apoptotic effect during persistent infection. We hypothesize that the accumulation of exogenous chlamydial HSP60 in the cytoplasm of actively replicating eukaryotic cells may interfere with the regulation of the apoptotic pathway. The concomitant expression of viral oncoproteins and/or the presence of mutations may lead to the ability to survive apoptotic stimuli, loss of replicative senescence, uncontrolled proliferation and, finally neoplastic transformation.Received 15 August 2004; received after revision 1 October 2004; accepted 7 October 2004     

基于图像块分割及小波空间频率的多聚焦图像融合法

提出了一种基于图像块分割及小波空间频率的多聚焦图像融合算法.采用块区域局部小波空间频率作为图像是否清晰的判断依据,对于明显的清晰和模糊区域,直接选取清晰区域作为融合后的相应块区域;而对于清晰与模糊的边界区域,采用了基于像素的窗口空问频率的方法进行融合处理.实验对比结果表明,该方法明显优于小波变换方法和图像块方法.