Male mouse submaxillary gland secretes highly toxic proteins

Summary Submaxillary gland saliva induced by phenylephrine from male mice was highly toxic to guinea-pigs, rats and hamsters, whereas the toxicity was relatively low to mice. One of the toxic components in the saliva was isolated as a kallikrein-like enzyme.     

Degradation of bacterial lipopolysaccharide by gut juice os the snail Helix pomatia.

Lipopolysaccharides from several bacteria were selectively degarded by gut juice of the snail Helix pomatia with extensive loss of anticomplementary activity and changes in the electrophoretic pattern in polyacrylamide gels. The gut juice had little effect on ketodeoxyoctonate content or immunodominant sugars. The lipid A moiety of the lipopolysaccharide appeared to be the main site of attack.     

Compared expression of murine plasmacytoma associated virus in tumor cells and in mouse embryo cells]

A DNA complementary to the viral genome of C-type particles produced by a Mouse myeloma derived cell line (MF2 cell line) was synthesized. This cDNA was used as a probe to study the viral genome expression among the total RNA and the poly (A)-rich RNA extracted from the MF2 and Balb/c embryonic cells. As evidenced by molecular hybridization experiments, the presence of at least one endogenous Balb/c virus in the MF2 virus stocks is suggested. In the productive cells, the viral RNA sequences are expressed in the poly (A)-rich RNA fraction.     

Accumulation of Phosvel in adipose tissue of hens

Summary Phosvel, an organophosphorus pesticide, was stored in the adipose tissue of hens after they were given daily a single oral dose. The concentration of Phosvel in fat was related to the size of the daily dose.Acknowledgment. The authors are grateful to Prof. Yoshito Tsuji for his valuable suggestions; to Mr Mitsuo Kaneda and Mr Kazuo Sasaki for their technical assistance.     

Monoclonal antibodies to the acetylcholine receptor by a normally functioning auto-anti-idiotypic mechanism

Recently we described a procedure for preparing antibodies to the acetylcholine receptor (AChR) based on immunoglobulin idiotypes and on the hypothesis that, regardless of functional differences, macromolecules of the same specificity will show structural homologies in their binding sites. Antibodies were prepared in rabbits to a structurally constrained agonist of AChR, trans-3,3'-bis[alpha-(trimethylammonio)methyl]azobenzene bromide (BisQ). These antibodies mimicked the binding specificity of AChR in its activated state--agonists were bound with affinities that were in accord with their biological activities and antagonists were bound poorly. Rabbits were then immunized with a specifically purified preparation of anti-BisQ to elicit a population of antibodies specific for the binding sites of anti-BisQ. A portion of the anti-idiotypic antibodies produced in the second set of rabbits cross-reacted with determinants on AChR preparations from Torpedo californica, Electrophorus electricus and rat muscle. Moreover, several of the rabbits showed signs of experimental myasthenia gravis, in which circulating AChR antibodies are typically found. To devise a more direct route to monoclonal anti-receptor antibodies we based our strategy on acceptance of the concept of the anti-idiotypic network theory of Jerne. According to this theory, injection of an antigen elicits, in addition to antibodies to the antigen, other populations that include anti-idiotypic antibodies directed at the combining sites of the antigen-specific antibodies. If the antigen-specific antibodies recognize a ligand of a receptor, then the anti-idiotypic antibodies should bind receptor. Thus, when a mouse is immunized with a bovine serum albumin conjugate of BisQ (BisQ-BSA), it should be possible to expand populations of spleen cells that secrete antibodies which bind anti-BisQ and AChR, in addition to populations specific for BisQ. Fusion of the spleen cells with an appropriate myeloma line should yield monoclonal anti-AChR antibodies. Here we report the success of this approach and its implications.