Lymphocyte reactivity in Imuran-treated guinea-pigs and in vitro effect of colchicine

Summary Imuran treatment significantly diminished macrophage migration inhibition in immunized guinea-pigs and the percentage of E-rosette forming cells in all treated animals. 10^–6 M colchicine in vitro significantly increased the percentage of E-rosette forming cells in Imuran-treated animals. The results suggest possible alteration of the lymphocyte receptors after treatment with Imuran.This work was supported by the grants of the Republic Research Fund of Serbia.     

Reinke's crystals at damaged germinal epithelium

Zusammenfassung Überprüfung der Reinkeschen Kristalle in den Leydingschen Zellen an einem bioptischen Material von 220 Hoden steriler Männer (22–45) entsprechend verschiedener Beschädigungsstufen des Samenepithels ergab keinen Einfluss des Keimepithels auf die Kristallbildung.

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Reinke, Arch. mikrosk. Anat. EntwMech.47, 34 (1896).     

A comparative study of the calorigenic action of noradrenaline in the rat and ground squirrel adapted to different temperatures

Résumé La capacité de produire de la chaleur sous l'influence de la même quantité de noradrénaline est beaucoup plus élevée chez le Spermophile que chez le rat. D'autre part, le séjour prolongé à la température de 36°C-contrairement à ce qui se passe chez les rats-ne réduit pas la sensibilité à la noradrénaline chez les Spermophiles examinés en octobre.     

Effect of a growth-promoting factor from calf muscles on the weight gain of hypophysectomized rats

Zusammenfassung Aus Kalbsmuskel wurde nach Sephadex G-25 Chromatographie jene Fraktion gereinigt, die als Sulfationsfaktor bekannt ist und den³⁵S-Sulfateinbau im embryonalen Hühnerknorpel in vitro steigert. Der Faktor kann bei hypophysektomierten Ratten die Wirkung von submaximalen Dosen des menschlichen Wachstumshormons erhöhen.     

Hemmung von Morphin-Effekten durch synthetische Substanz P

Summary Synthetic Substance P and naloxone abolish the proconvulsive action of morphine on pentetrasol-induced seizures. It is suggested that Substance P could be a natural antagonist of morphine in the central nervous system.

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Am 20. März 1976 gestorben.     

Hypertension mediated by the activation of the rat brain 5-hydroxytryptamine receptor sites

Summary 5-Hydroxytryptamine (5-HT) administered intraventricularly (i.vent.) in rats produced hypertension without considerable changes in heart rate. After transsection of the spinal cord or i.vent. administration of methysergide, 5-HT failed to produce the pressor effect. Thus, the hypertension results from the activation of 5-HT receptor sites of the rat brain.This work was supported by Union of Scientific Medical Institutions of Serbia (Grant No. 85).     

Secretory phenomenon of the adenohypophyseal cells viewed with the scanning electron microscope

Summary Exocytosis from rat adenohypophyseal cells, probably somatotrophic, was studied in the scanning electron microscope after freeze fracturation of the glands. This technique permitted the distinguishing of a clearly delimited exoplasm interrupted by the passage of the secretory granules. It was postulated that the exoplasm could be involved in the control of the release of secretory granules in the endocrine cells studied. At the basal cell surface a simultaneous discharge of several secretory granules has been detected.With the technical assistance of Mr P.-A. Milliquet.     

A novel xanthone as secondary metabolite fromCentaurium cachanlahuen

Summary From the plantCentaurium cachanlahuen, swertiaperenine1, swercherine2, decusatine4, oleanolic acid5 and the xanthone 1,8-dihydroxy-2,3,4,6-tetramethoxy-9H-xanthone3 were isolated and characterized.     

Estradiol-induced changes in TSH-like immunoreactivity of pituitary cells in female rats

Beta-thyrotropin (TSH)-producing cells in the pituitary pars distalis of female rats were studied using rabbit anti-rat beta-thyrotropin (TSH) serum and a peroxidase-antiperoxidase (PAP) immunohistochemical procedure. Animals were neonatally treated with 1 mg estradiol-dipropionate (EDP) and sacrificed at different stages of development up to adulthood. Intact females of the corresponding age served as the controls. Morphometry and stereology were used to evaluate the changes in TSH-cell number and volume densities of the cells and nuclei. All morphometric parameters examined in estradiol-treated animals showed a significant decrease in comparison with immunoreactive TSH cells of age-matched controls. The most prominent EDP-induced changes were evident in peripubertal 38-day-old rats, the number and volumetric densities of both TSH cells and their nuclei being reduced by about 90% compared to intact pituitary. This decrease in the number and volume densities of TSH cells in EDP-treated rats explicitly demonstrated that this hormone, applied neonatally, has an inhibitory effect on TSH-immunoreactive cells up to adulthood, in accordance with our earlier data obtained by light and electron microscopy.