The accessory salivary gland as the source of water in the saliva of hemiptera: Heteroptera

Summary Ablation of the accessory salivary glands of a pentatomid and of a coreid reduced quantitatively the flow of alkaline, watery saliva that can be induced by topical application of pilocarpine.     

DISC1-dependent switch from progenitor proliferation to migration in the developing cortex

Regulatory mechanisms governing the sequence from progenitor cell proliferation to neuronal migration during corticogenesis are poorly understood. Here we report that phosphorylation of DISC1, a major susceptibility factor for several mental disorders, acts as a molecular switch from maintaining proliferation of mitotic progenitor cells to activating migration of postmitotic neurons in mice. Unphosphorylated DISC1 regulates canonical Wnt signalling via an interaction with GSK3β, whereas specific phosphorylation at serine 710 (S710) triggers the recruitment of Bardet-Biedl syndrome (BBS) proteins to the centrosome. In support of this model, loss of BBS1 leads to defects in migration, but not proliferation, whereas DISC1 knockdown leads to deficits in both. A phospho-dead mutant can only rescue proliferation, whereas a phospho-mimic mutant rescues exclusively migration defects. These data highlight a dual role for DISC1 in corticogenesis and indicate that phosphorylation of this protein at S710 activates a key developmental switch.     

Peptide sequences of T-cell receptor delta and gamma chains are identical to predicted X and gamma proteins

Although most mature peripheral T lymphocytes express a major histocompatibility complex restricted, CD3-associated, antigen receptor (TCR) which has been well characterized, some T cells carry a different CD3-associated heterodimer on their surface. One of the two disulphide-linked chains of this putative second receptor, which in mice has relative molecular mass (Mr) 35,000 (35K), has been identified as a product of the group of gamma genes. The other chain, termed delta (Mr 45K in mice), is not as well characterized. Although gamma/delta-bearing cells are a minor subset among peripheral T lymphocytes, they are the only CD3+ cells in the thymus early in ontogeny. Taking advantage of these kinetics, we have generated gamma/delta-bearing hybridomas, using a new TCR alpha chain-negative variant of the AKR thymoma BW5147 as tumour parent, fetal thymocytes as normal cell partners, and an anti-CD3 monoclonal antibody (mAb) as screening reagent. Gamma and delta chains from one of these hybrids have been purified and partially sequenced. The sequences obtained indicate that delta is indeed identical to the polypeptide encoded by the recently described gene X, as suggested by Chien et al.     

Influence of scene statistics on colour constancy

The light reflected from an object depends not only on the surface properties of this object but also on the illuminant. The same is true for the excitations of the photoreceptors, which serve as the basis for the perceived colour. However, our visual system has the ability to perceive constant surface colours despite changes in illumination. The average chromaticity of the retinal image of a scene depends on the illumination, and thus might be used by the visual system to estimate the illumination and to modulate the correction that subserves colour constancy. But this measure is not sufficient: a reddish scene under white light can produce the same mean stimulation as a neutral scene in red light. Higher order scene statistics-for example, the correlation between redness and luminance within the image-allow these cases to be distinguished. Here we report that the human visual system does exploit such a statistic when estimating the illuminant, and gives it a weight that is statistically appropriate for the natural environment.     

Antibiotic sensitivity testing by flow microcalorimetry

Summary The proposed flow microcalorimetric method for the diagnosis of bacteriuria has been extended to include antibiotic sensitivity testing. Sensitive organisms rapidly (4–8 min) show thermal responses to the added antibiotics over the normal range of concentrations (1×, 2×, MIC value).