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71.
U.S. Fish and Wildlife Service staff at Hart Mountain National Antelope Refuge (HMNAR), southeastern Oregon, documented high pronghorn ( Antilocapra americana ) fawn mortality, subsequent low fawn recruitment, and declining pronghorn numbers from 1996 to 1999. Coyote ( Canis latrans ) predation was the primary cause, accounting for 60-85% of fawn mortalities each year, and fawns were not physiologically predisposed to predation. Therefore, we investigated certain coyote population parameters (age structure, survival, density, physiology) to evaluate how or if these factors influence coyote predation rates on pronghorn fawns. We captured 11 coyotes (5 male and 6 female) in December 1998. Age of captured animals ranged from 1.7 to 10.7 yrs ( ̄x = 5.0 years), and all coyotes appeared healthy upon capture. There were no known mortalities through December 1999. We estimated pre-whelping (December through February 1997-1999) density from howling surveys conducted within HMNAR to be 0.40-0.53 km -2 . Compared to other published studies, we found significant ( P ≤ 0.05) differences in selected blood parameters (e.g., blood urea nitrogen, total protein, white blood cell counts), indicating coyote nutrition may be marginal to deficient during winter at HMNAR. A high percentage of coyotes (91%) tested positive for serum-neutralizing antibodies to canine parvovirus. We judged that parasite ( Toxascaris spp., Alaria spp., Sarcocystis spp., and Isospora spp.) prevalence and intensity were not high enough to influence coyote condition. Based on our data, the coyote population at HMNAR is old aged, at a relatively high density, and stable, but their nutrition may be marginal to deficient during winter. Presently, we are unable to draw direct conclusions relating the parameters we sampled with predation rates by this unexploited coyote population.  相似文献   
72.
Revealing the underlying preferences of a forecaster has always been at the core of much controversy. Herein, we build on the multivariate loss function concept and propose a flexible and multivariate family of likelihoods. This allows examining whether a vector of forecast errors, along with control variables, shapes a forecaster's preferences and, therefore, the underlying multivariate, nonseparable, loss function. We estimate the likelihood function using Bayesian exponentially tilted empirical likelihood, which reveals the shape of the parameter and the power of the multivariate loss function. In the empirical section, the reported evidence reveals that the EU Commission forecasts are predominantly asymmetric, leaning towards optimism in the year ahead, while a correction towards pessimism occurs in the current year forecast. There is some variability of this asymmetry across member states, with forecasts, i.e. gross domestic product growth, for large Member States exhibiting more optimism  相似文献   
73.
Clar.  JH Lanca.  M 《自然杂志》2000,22(1):1-6
绿色化学是化学工业走向可持续发展和具有竞争性的有效途径,并日益为政府、工业界及公众接受为社会、经济及环境发展的必要目标.本文对绿色化学的概念、进展及实践作了评介.  相似文献   
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75.
Anaerobic ammonium oxidation (anammox) has become a main focus in oceanography and wastewater treatment. It is also the nitrogen cycle's major remaining biochemical enigma. Among its features, the occurrence of hydrazine as a free intermediate of catabolism, the biosynthesis of ladderane lipids and the role of cytoplasm differentiation are unique in biology. Here we use environmental genomics--the reconstruction of genomic data directly from the environment--to assemble the genome of the uncultured anammox bacterium Kuenenia stuttgartiensis from a complex bioreactor community. The genome data illuminate the evolutionary history of the Planctomycetes and allow us to expose the genetic blueprint of the organism's special properties. Most significantly, we identified candidate genes responsible for ladderane biosynthesis and biological hydrazine metabolism, and discovered unexpected metabolic versatility.  相似文献   
76.
A resource for large-scale RNA-interference-based screens in mammals   总被引:2,自引:0,他引:2  
Gene silencing by RNA interference (RNAi) in mammalian cells using small interfering RNAs (siRNAs) and short hairpin RNAs (shRNAs) has become a valuable genetic tool. Here, we report the construction and application of a shRNA expression library targeting 9,610 human and 5,563 mouse genes. This library is presently composed of about 28,000 sequence-verified shRNA expression cassettes contained within multi-functional vectors, which permit shRNA cassettes to be packaged in retroviruses, tracked in mixed cell populations by means of DNA 'bar codes', and shuttled to customized vectors by bacterial mating. In order to validate the library, we used a genetic screen designed to report defects in human proteasome function. Our results suggest that our large-scale RNAi library can be used in specific, genetic applications in mammals, and will become a valuable resource for gene analysis and discovery.  相似文献   
77.
This paper is a rare example of a large scale (n = 1310), positivist, evaluation of an action research program. It documents how the Royal Navy used the principles of scholarly consulting and pragmatic science to develop, apply and review a systems based tool, and associated ‘new organisation development’ intervention, to help staff explore and share perceptions of working practices, in order to expand their awareness of their current work situation and so uncover opportunities for improvement. Survey results suggest that the majority of individuals found the interventions valuable and participants in the events enjoyed significantly higher levels of understanding of the organisation, and greater collective, and individual, benefit from it. Findings also provide empirical evidence of the importance of involvement to making successful change, especially when dealing with the change averse. Overall the experience, of which the action research based study and associated positivist survey were part, reinforce the importance of what is described as ‘normative realigning pedagogy’—helping people change by facilitating their generation of new forms of understanding.  相似文献   
78.
The idea of the global manufacturing virtual network (GMVN) was introduced in 2000 in order to highlight an emerging as well as an alternative manufacturing system which will have strong impacts on industry and implications to management theory. During the last three years, more than thirty companies - based in the electronics, bio-pharmaceuticals, garment, and home electronics appliance industries - have been studied in the UK and China. The research project addresses the emergence of the collaborative manufacturing phenomenon at three levels - sector, system and enabling technology. This paper summarises preliminary research findings from fieldwork conducted over the last three years. It seeks to clarify the characteristics and functionality of GMVN through the case studies and to contrast it to other types of business model, such as the multinational corporations (MNC) and international strategic alliances (ISAs). It also raises some new research questions and themes for further research into GMVN.  相似文献   
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80.
Bananas (Musa spp.), including dessert and cooking types, are giant perennial monocotyledonous herbs of the order Zingiberales, a sister group to the well-studied Poales, which include cereals. Bananas are vital for food security in many tropical and subtropical countries and the most popular fruit in industrialized countries. The Musa domestication process started some 7,000 years ago in Southeast Asia. It involved hybridizations between diverse species and subspecies, fostered by human migrations, and selection of diploid and triploid seedless, parthenocarpic hybrids thereafter widely dispersed by vegetative propagation. Half of the current production relies on somaclones derived from a single triploid genotype (Cavendish). Pests and diseases have gradually become adapted, representing an imminent danger for global banana production. Here we describe the draft sequence of the 523-megabase genome of a Musa acuminata doubled-haploid genotype, providing a crucial stepping-stone for genetic improvement of banana. We detected three rounds of whole-genome duplications in the Musa lineage, independently of those previously described in the Poales lineage and the one we detected in the Arecales lineage. This first monocotyledon high-continuity whole-genome sequence reported outside Poales represents an essential bridge for comparative genome analysis in plants. As such, it clarifies commelinid-monocotyledon phylogenetic relationships, reveals Poaceae-specific features and has led to the discovery of conserved non-coding sequences predating monocotyledon-eudicotyledon divergence.  相似文献   
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