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91.
Summary A new method for autoradiographic screening of amplified genes in cellular clones is described. The main feature of the device is to keep viable cells from each clone, which can subsequently be regrown. The availability of this biochemical screening method allows screening for recombinants harboring unselectable markers as well. 相似文献
92.
Constrained Latent Class Analysis of Three-Way Three-Mode Data 总被引:1,自引:1,他引:0
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94.
I. Balla C. Michel Hedi Fritz-Niggli 《Cellular and molecular life sciences : CMLS》1981,37(10):1105-1106
Summary The eye region of mouse embryos, irradiated with 90 rad X-rays on day 8 of gestation, was examined 24 h later for cellular damage. Besides the overall developmental retardation, the radiation insult decreased the proliferation rate and altered the mitotic phase ratio. Due to the limited extension of necrotic zones, a reduced number of dead cells was found in the irradiated optic vesicles.Supported by the Swiss National Foundation for Scientific Research. The technical assistance of Mrs E. Frei is gratefully acknowledged. 相似文献
95.
C Michel 《Experientia》1974,30(10):1195-1196
96.
S Naltchayan J Bouhnik R Michel 《Comptes rendus des séances de l'Académie des sciences. Série D, Sciences naturelles》1978,287(11):1047-1049
Serum concentrations of T4 and T3 in 2 year-old Rats were decreased by about 30% compared to 2 month-old animals, but rT3 was similar. Activities of old Rats' liver mitochondria were near those of young thyroidectomized animals. Low circulating T3 partly explains metabolic changes observed during senescence. 相似文献
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99.
Michel Puech 《Foundations of Science》2013,18(2):351-353
This commentary on Gert Goeminne’s paper “Postphenomenology and the politics of sustainable technology” elaborates on the subpolitics of technology as a basis for dealing with sustainability issues. It questions the “sustainable technology” phrasing of the issue and focuses on the political/post-political debate to eventually suggest that the politics of sustainable technology is a possible post-political question. Minor disagreements on some philosophy of science references are briefly expressed. 相似文献
100.
Hakim O Resch W Yamane A Klein I Kieffer-Kwon KR Jankovic M Oliveira T Bothmer A Voss TC Ansarah-Sobrinho C Mathe E Liang G Cobell J Nakahashi H Robbiani DF Nussenzweig A Hager GL Nussenzweig MC Casellas R 《Nature》2012,484(7392):69-74
Recurrent chromosomal translocations underlie both haematopoietic and solid tumours. Their origin has been ascribed to selection of random rearrangements, targeted DNA damage, or frequent nuclear interactions between translocation partners; however, the relative contribution of each of these elements has not been measured directly or on a large scale. Here we examine the role of nuclear architecture and frequency of DNA damage in the genesis of chromosomal translocations by measuring these parameters simultaneously in cultured mouse B lymphocytes. In the absence of recurrent DNA damage, translocations between Igh or Myc and all other genes are directly related to their contact frequency. Conversely, translocations associated with recurrent site-directed DNA damage are proportional to the rate of DNA break formation, as measured by replication protein A accumulation at the site of damage. Thus, non-targeted rearrangements reflect nuclear organization whereas DNA break formation governs the location and frequency of recurrent translocations, including those driving B-cell malignancies. 相似文献