全文获取类型
收费全文 | 2122篇 |
免费 | 9篇 |
国内免费 | 18篇 |
专业分类
系统科学 | 35篇 |
丛书文集 | 2篇 |
教育与普及 | 11篇 |
理论与方法论 | 49篇 |
现状及发展 | 258篇 |
研究方法 | 329篇 |
综合类 | 1345篇 |
自然研究 | 120篇 |
出版年
2021年 | 7篇 |
2020年 | 5篇 |
2019年 | 4篇 |
2018年 | 17篇 |
2017年 | 20篇 |
2016年 | 21篇 |
2015年 | 15篇 |
2014年 | 20篇 |
2013年 | 28篇 |
2012年 | 181篇 |
2011年 | 313篇 |
2010年 | 75篇 |
2009年 | 14篇 |
2008年 | 147篇 |
2007年 | 207篇 |
2006年 | 168篇 |
2005年 | 188篇 |
2004年 | 188篇 |
2003年 | 163篇 |
2002年 | 170篇 |
2001年 | 12篇 |
2000年 | 18篇 |
1999年 | 14篇 |
1997年 | 6篇 |
1995年 | 5篇 |
1994年 | 6篇 |
1993年 | 8篇 |
1992年 | 11篇 |
1991年 | 4篇 |
1990年 | 8篇 |
1989年 | 4篇 |
1988年 | 8篇 |
1987年 | 6篇 |
1986年 | 5篇 |
1985年 | 4篇 |
1984年 | 3篇 |
1983年 | 3篇 |
1982年 | 7篇 |
1979年 | 5篇 |
1978年 | 4篇 |
1977年 | 3篇 |
1976年 | 5篇 |
1974年 | 3篇 |
1973年 | 4篇 |
1972年 | 5篇 |
1971年 | 4篇 |
1970年 | 3篇 |
1969年 | 6篇 |
1968年 | 5篇 |
1967年 | 4篇 |
排序方式: 共有2149条查询结果,搜索用时 31 毫秒
181.
The geochemical composition of the Earth's upper mantle is thought to reflect 4.5 billion years of melt extraction, as well as the recycling of crustal materials. The fractionation of rhenium and osmium during partial melting in the upper mantle makes the Re-Os isotopic system well suited for tracing the extraction of melt and recycling of the resulting mid-ocean-ridge basalt. Here we report osmium isotope compositions of more than 700 osmium-rich platinum-group element alloys derived from the upper mantle. The osmium isotopic data form a wide, essentially gaussian distribution, demonstrating that, with respect to Re-Os isotope systematics, the upper mantle is extremely heterogeneous. As depleted and enriched domains can apparently remain unequilibrated on a timescale of billions of years, effective equilibration seems to require high degrees of partial melting, such as occur under mid-ocean ridges or in back-arc settings, where percolating melts enhance the mobility of both osmium and rhenium. We infer that the gaussian shape of the osmium isotope distribution is the signature of a random mixing process between depleted and enriched domains, resulting from a 'plum pudding' distribution in the upper mantle, rather than from individual melt depletion events. 相似文献
182.
183.
A bacteriolytic agent that detects and kills Bacillus anthracis 总被引:17,自引:0,他引:17
The dormant and durable spore form of Bacillus anthracis is an ideal biological weapon of mass destruction. Once inhaled, spores are transported by alveolar macrophages to lymph nodes surrounding the lungs, where they germinate; subsequent vegetative expansion causes an overwhelming flood of bacteria and toxins into the blood, killing up to 99% of untreated victims. Natural and genetically engineered antibiotic-resistant bacilli amplify the threat of spores being used as weapons, and heighten the need for improved treatments and spore-detection methods after an intentional release. We exploited the inherent binding specificity and lytic action of bacteriophage enzymes called lysins for the rapid detection and killing of B. anthracis. Here we show that the PlyG lysin, isolated from the gamma phage of B. anthracis, specifically kills B. anthracis isolates and other members of the B. anthracis 'cluster' of bacilli in vitro and in vivo. Both vegetative cells and germinating spores are susceptible. The lytic specificity of PlyG was also exploited as part of a rapid method for the identification of B. anthracis. We conclude that PlyG is a tool for the treatment and detection of B. anthracis. 相似文献
184.
Bird AW Yu DY Pray-Grant MG Qiu Q Harmon KE Megee PC Grant PA Smith MM Christman MF 《Nature》2002,419(6905):411-415
185.
186.
187.
Hon WC Wilson MI Harlos K Claridge TD Schofield CJ Pugh CW Maxwell PH Ratcliffe PJ Stuart DI Jones EY 《Nature》2002,417(6892):975-978
188.
Widespread use of antimalarial agents can profoundly influence the evolution of the human malaria parasite Plasmodium falciparum. Recent selective sweeps for drug-resistant genotypes may have restricted the genetic diversity of this parasite, resembling effects attributed in current debates to a historic population bottleneck. Chloroquine-resistant (CQR) parasites were initially reported about 45 years ago from two foci in southeast Asia and South America, but the number of CQR founder mutations and the impact of chlorquine on parasite genomes worldwide have been difficult to evaluate. Using 342 highly polymorphic microsatellite markers from a genetic map, here we show that the level of genetic diversity varies substantially among different regions of the parasite genome, revealing extensive linkage disequilibrium surrounding the key CQR gene pfcrt and at least four CQR founder events. This disequilibrium and its decay rate in the pfcrt-flanking region are consistent with strong directional selective sweeps occurring over only approximately 20-80 sexual generations, especially a single resistant pfcrt haplotype spreading to very high frequencies throughout most of Asia and Africa. The presence of linkage disequilibrium provides a basis for mapping genes under drug selection in P. falciparum. 相似文献
189.
190.
Stöck M Lamatsch DK Steinlein C Epplen JT Grosse WR Hock R Klapperstück T Lampert KP Scheer U Schmid M Schartl M 《Nature genetics》2002,30(3):325-328
Green toads are common in the Palaearctic region, where they have differentiated into several taxa. The toads exist with variable amounts of ploidy, similar to other anuran species or reptiles. In vertebrate biology, the very rare occurrence of triploidy is coupled with infertility or unisexuality, or requires the coexistence of individuals of different ploidy in a reproductive community. The reproduction of naturally occurring triploids has been reported to occur only through parthenogenesis, gynogenesis or hybridogenesis. The bisexual reproduction of pure triploids has been considered to be impossible because of the problem of equally distributing three chromosome sets in meiosis. Here we report geographically isolated populations of green toads (Bufo viridis complex) that are all-triploid and reproduce bisexually. 相似文献