排序方式: 共有84条查询结果,搜索用时 15 毫秒
61.
Marina El Haddad Cécile Notarnicola Brendan Evano Nour El Khatib Marine Blaquière Anne Bonnieu Shahragim Tajbakhsh Gérald Hugon Barbara Vernus Jacques Mercier Gilles Carnac 《Cellular and molecular life sciences : CMLS》2017,74(10):1923-1936
Muscle satellite cells are resistant to cytotoxic agents, and they express several genes that confer resistance to stress, thus allowing efficient dystrophic muscle regeneration after transplantation. However, once they are activated, this capacity to resist to aggressive agents is diminished resulting in massive death of transplanted cells. Although cell immaturity represents a survival advantage, the signalling pathways involved in the control of the immature state remain to be explored. Here, we show that incubation of human myoblasts with retinoic acid impairs skeletal muscle differentiation through activation of the retinoic-acid receptor family of nuclear receptor. Conversely, pharmacologic or genetic inactivation of endogenous retinoic-acid receptors improved myoblast differentiation. Retinoic acid inhibits the expression of early and late muscle differentiation markers and enhances the expression of myogenic specification genes, such as PAX7 and PAX3. These results suggest that the retinoic-acid-signalling pathway might maintain myoblasts in an undifferentiated/immature stage. To determine the relevance of these observations, we characterised the retinoic-acid-signalling pathways in freshly isolated satellite cells in mice and in siMYOD immature human myoblasts. Our analysis reveals that the immature state of muscle progenitors is correlated with high expression of several genes of the retinoic-acid-signalling pathway both in mice and in human. Taken together, our data provide evidences for an important role of the retinoic-acid-signalling pathway in the regulation of the immature state of muscle progenitors. 相似文献
62.
Luis A. Cea Carlos Puebla Bruno A. Cisterna Rosalba Escamilla Aníbal A. Vargas Marina Frank Paloma Martínez-Montero Carmen Prior Jesús Molano Isabel Esteban-Rodríguez Ignacio Pascual Pía Gallano Gustavo Lorenzo Héctor Pian Luis C. Barrio Klaus Willecke Juan C. Sáez 《Cellular and molecular life sciences : CMLS》2016,73(13):2583-2599
63.
DT Jones N Jäger M Kool T Zichner B Hutter M Sultan YJ Cho TJ Pugh V Hovestadt AM Stütz T Rausch HJ Warnatz M Ryzhova S Bender D Sturm S Pleier H Cin E Pfaff L Sieber A Wittmann M Remke H Witt S Hutter T Tzaridis J Weischenfeldt B Raeder M Avci V Amstislavskiy M Zapatka UD Weber Q Wang B Lasitschka CC Bartholomae M Schmidt C von Kalle V Ast C Lawerenz J Eils R Kabbe V Benes P van Sluis J Koster R Volckmann D Shih MJ Betts RB Russell S Coco GP Tonini U Schüller V Hans N Graf YJ Kim C Monoranu 《Nature》2012,488(7409):100-105
Medulloblastoma is an aggressively growing tumour, arising in the cerebellum or medulla/brain stem. It is the most common malignant brain tumour in children, and shows tremendous biological and clinical heterogeneity. Despite recent treatment advances, approximately 40% of children experience tumour recurrence, and 30% will die from their disease. Those who survive often have a significantly reduced quality of life. Four tumour subgroups with distinct clinical, biological and genetic profiles are currently identified. WNT tumours, showing activated wingless pathway signalling, carry a favourable prognosis under current treatment regimens. SHH tumours show hedgehog pathway activation, and have an intermediate prognosis. Group 3 and 4 tumours are molecularly less well characterized, and also present the greatest clinical challenges. The full repertoire of genetic events driving this distinction, however, remains unclear. Here we describe an integrative deep-sequencing analysis of 125 tumour-normal pairs, conducted as part of the International Cancer Genome Consortium (ICGC) PedBrain Tumor Project. Tetraploidy was identified as a frequent early event in Group 3 and 4 tumours, and a positive correlation between patient age and mutation rate was observed. Several recurrent mutations were identified, both in known medulloblastoma-related genes (CTNNB1, PTCH1, MLL2, SMARCA4) and in genes not previously linked to this tumour (DDX3X, CTDNEP1, KDM6A, TBR1), often in subgroup-specific patterns. RNA sequencing confirmed these alterations, and revealed the expression of what are, to our knowledge, the first medulloblastoma fusion genes identified. Chromatin modifiers were frequently altered across all subgroups. These findings enhance our understanding of the genomic complexity and heterogeneity underlying medulloblastoma, and provide several potential targets for new therapeutics, especially for Group 3 and 4 patients. 相似文献
64.
Hormonal control of cold stress responses in plants 总被引:1,自引:0,他引:1
65.
Marta Ruiz-Guillen Evgeni Gabev Jose I. Quetglas Erkuden Casales María Cristina Ballesteros-Briones Joanna Poutou Alejandro Aranda Eva Martisova Jaione Bezunartea Marina Ondiviela Jesus Prieto Ruben Hernandez-Alcoceba Nicola G. A. Abrescia Cristian Smerdou 《Cellular and molecular life sciences : CMLS》2016,73(20):3897-3916
66.
Diola Marina Núñez-Ramírez Aquiles Solís-Soto Javier López-Miranda Benito Pereyra-Alférez Miriam Rutiaga-Quiñónes Luis Medina-Torres Hiram Medrano-Roldán 《矿物冶金与材料学报》2011,18(5):523-526
The iron concentrate from Hercules Mine of Coahuila, Mexico, which mainly contained pyrite and pyrrhotite, was treated by
the bioleaching process using native strain Acidithiobacillus ferrooxidans (A. ferrooxidans) to determine the ability of these bacteria on the leaching of zinc. The native bacteria were isolated from the iron concentrate
of the mine. The bioleaching experiments were carried out in shake flasks to analyze the effects of pH values, pulp density,
and the ferrous sulfate concentration on the bioleaching process. The results obtained by microbial kinetic analyses for the
evaluation of some aspects of zinc leaching show that the native bacteria A. ferrooxidans, which is enriched with a 9K Silverman medium under the optimum conditions of pH 2.0, 20 g/L pulp density, and 40 g/L FeSO4, increases the zinc extraction considerably observed by monitoring during15 d, i.e., the zinc concentration has a decrease of about 95% in the iron concentrate. 相似文献
67.
Kiave-Yune HoWangYin Estibaliz Alegre Marina Daouya Benoit Favier Edgardo D. Carosella Joel LeMaoult 《Cellular and molecular life sciences : CMLS》2010,67(7):1133-1145
Trogocytosis is the uptake of membranes from one cell by another. Trogocytosis has been demonstrated for monocytes, B cells,
T cells, and NK cells. The acquisition of the tolerogenic molecule HLA-G by T cells and NK cells makes them behave as regulatory
cells. We investigated here whether HLA-G, which is expressed by tumor cells in vivo, could be acquired by monocytes and if
this transfer could have functional consequences. We demonstrate that resting, and even more so, activated monocytes efficiently
acquire membrane-bound HLA-G from HLA-G tumor cells by trogocytosis. However, we demonstrate that HLA-G quickly disappears
from the surface of the monocytes in contrast to the HLA-G acquired by T cells. Consequently, HLA-Gacq+ monocytes do not reliably inhibit the on-going proliferation of autologous activated T cells and do not inhibit their cytokine
production. Thus, we show that the acquirer cell may control the functional outcome of trogocytosis. 相似文献
68.
Tubulin chaperone E binds microtubules and proteasomes and protects against misfolded protein stress
Olga Voloshin Yana Gocheva Marina Gutnick Natalia Movshovich Anya Bakhrat Keren Baranes-Bachar Dudy Bar-Zvi Ruti Parvari Larisa Gheber Dina Raveh 《Cellular and molecular life sciences : CMLS》2010,67(12):2025-2038
Mutation of tubulin chaperone E (TBCE) underlies hypoparathyroidism, retardation, and dysmorphism (HRD) syndrome with defective
microtubule (MT) cytoskeleton. TBCE/yeast Pac2 comprises CAP-Gly, LRR (leucine-rich region), and UbL (ubiquitin-like) domains.
TBCE folds α-tubulin and promotes α/β dimerization. We show that Pac2 functions in MT dynamics: the CAP-Gly domain binds α-tubulin
and MTs, and functions in suppression of benomyl sensitivity of pac2Δ mutants. Pac2 binds proteasomes: the LRR binds Rpn1, and the UbL binds Rpn10; the latter interaction mediates Pac2 turnover.
The UbL also binds the Skp1-Cdc53-F-box (SCF) ubiquitin ligase complex; these competing interactions for the UbL may impact
on MT dynamics. pac2Δ mutants are sensitive to misfolded protein stress. This is suppressed by ectopic PAC2 with both the CAP-Gly and UbL domains being essential. We propose a novel role for Pac2 in the misfolded protein stress response
based on its ability to interact with both the MT cytoskeleton and the proteasomes. 相似文献
69.
Rees MI Harvey K Pearce BR Chung SK Duguid IC Thomas P Beatty S Graham GE Armstrong L Shiang R Abbott KJ Zuberi SM Stephenson JB Owen MJ Tijssen MA van den Maagdenberg AM Smart TG Supplisson S Harvey RJ 《Nature genetics》2006,38(7):801-806
Hyperekplexia is a human neurological disorder characterized by an excessive startle response and is typically caused by missense and nonsense mutations in the gene encoding the inhibitory glycine receptor (GlyR) alpha1 subunit (GLRA1). Genetic heterogeneity has been confirmed in rare sporadic cases, with mutations affecting other postsynaptic glycinergic proteins including the GlyR beta subunit (GLRB), gephyrin (GPHN) and RhoGEF collybistin (ARHGEF9). However, many individuals diagnosed with sporadic hyperekplexia do not carry mutations in these genes. Here we show that missense, nonsense and frameshift mutations in SLC6A5 (ref. 8), encoding the presynaptic glycine transporter 2 (GlyT2), also cause hyperekplexia. Individuals with mutations in SLC6A5 present with hypertonia, an exaggerated startle response to tactile or acoustic stimuli, and life-threatening neonatal apnea episodes. SLC6A5 mutations result in defective subcellular GlyT2 localization, decreased glycine uptake or both, with selected mutations affecting predicted glycine and Na+ binding sites. 相似文献