高原鼢鼠油脂中油酸含量定量分析

用气象色谱和气象色谱-质谱联用方法对高原鼢鼠油脂中油酸含量进行了定量分析,并对方法的准确度进行了检验。结果发现,高原鼢鼠油脂中油酸含量为18.57%±0.43%,皂化和酯化最佳时间分别为15min和7min,油酸甲酯标准曲线回归系数r=0.9999,油酸甲酯色谱峰面积的相对偏差为0.0171,样品中油酸甲酯回收率为94.08%。     

SCI及其衍生产品与科研学术绩效评价

介绍了引文索引的功能及其异化过程,探讨了国内外科研学术绩效评价中引文数据的应用,分析了SCI及其衍生产品的科研绩效评价功能,阐述了SCI来源刊及其分区依据。     

三维虚拟现实技术下刚体转动实验的开发与应用

利用3DS MAX及Cult 3D软件操作平台,先建立三维虚拟的刚体转动实验仪器模型,再使用Cult 3D Designer编辑器,通过组织事件、动作与对象的关联编程,实现了该实验的复杂交互动作.完成基于交互式虚拟技术的刚体转动实验浏览与操纵系统.     

涡流无损检测淬火钢轨踏面硬度的数值分析

详细地介绍了一种利用电磁涡流信号检测淬火钢轨硬度的新型无损检测装置 .利用该装置可连续地实现钢轨踏面硬度的检测 ,并且通过应用计算机信号处理技术和图形技术 ,使得该装置的检测精度达到了工业测量的要求 .     

生物类似药在欧盟、美国和俄罗斯的机遇与挑战

正有关生物类似药机会的问题,监管层和生物制药行业已经谈论了十多年。如今,市场价值约1 000亿美元的生物药,包括多年占据全球畅销药首位的修美乐(Humira),将在未来几年里失去在美国和欧洲的关键专利保护;仅在美国,到2021年,预计将有70多个生物药专利到期。生物类似药的机会比以往任何时候都要更加巨大。     

First record of Orbopercularia lichtensteini (Peritrichia: Operculariidae) attached to a hemipteran,Corisella edulis (Nepomorpha: Corixidae), in Hidalgo,Mexico, with notes on its cytology and distributional patterns

Operculariidae includes sessile ciliate species mainly attached to diverse substrates, but records are scarce for their attachment to aquatic true bugs. The goal of this contribution is to provide new cytological data with optical and scanning electron microscopy of Orbopercularia lichtensteini, and some notes about their distribution on a Mexican corixid. We manually collected the corixids in a pond in Mexico during a six-month period, and all individuals were revised to observe the peritrichids. We calculated the abundance and prevalence, with an emphasis on the specific micro-location of the epibiotic peritrich on the host Corisella edulis. A total of 36,205 peritrichids were recorded in 158 corixids. The colonies with fine striated zooids were dichotomously branched and mostly attached to left eyes, but also on other body regions, i.e. left wings and rostrum. We conclude that some morphological colony traits bring some advantages for food particle capture, and aspects of the biology and morphology of Corisella edulis play an important role in determining the spatial distribution of O. lichtensteini.     

Functional genomics reveal that the serine synthesis pathway is essential in breast cancer

Cancer cells adapt their metabolic processes to drive macromolecular biosynthesis for rapid cell growth and proliferation. RNA interference (RNAi)-based loss-of-function screening has proven powerful for the identification of new and interesting cancer targets, and recent studies have used this technology in vivo to identify novel tumour suppressor genes. Here we developed a method for identifying novel cancer targets via negative-selection RNAi screening using a human breast cancer xenograft model at an orthotopic site in the mouse. Using this method, we screened a set of metabolic genes associated with aggressive breast cancer and stemness to identify those required for in vivo tumorigenesis. Among the genes identified, phosphoglycerate dehydrogenase (PHGDH) is in a genomic region of recurrent copy number gain in breast cancer and PHGDH protein levels are elevated in 70% of oestrogen receptor (ER)-negative breast cancers. PHGDH catalyses the first step in the serine biosynthesis pathway, and breast cancer cells with high PHGDH expression have increased serine synthesis flux. Suppression of PHGDH in cell lines with elevated PHGDH expression, but not in those without, causes a strong decrease in cell proliferation and a reduction in serine synthesis. We find that PHGDH suppression does not affect intracellular serine levels, but causes a drop in the levels of α-ketoglutarate, another output of the pathway and a tricarboxylic acid (TCA) cycle intermediate. In cells with high PHGDH expression, the serine synthesis pathway contributes approximately 50% of the total anaplerotic flux of glutamine into the TCA cycle. These results reveal that certain breast cancers are dependent upon increased serine pathway flux caused by PHGDH overexpression and demonstrate the utility of in vivo negative-selection RNAi screens for finding potential anticancer targets.     

产甘油重组大肠杆菌的构建及其耐高渗透压性能的测定

克隆了酿酒酵母(Saccharomyces cerevisiae) 中的3-磷酸甘油脱氢酶和3-磷酸甘油酯酶基因,通过融合PCR构建了双基因共表达载体,将酵母细胞内应答渗透压变化的甘油合成途径引入大肠杆菌（Escherichia coli）。以葡萄糖为底物对重组大肠杆菌进行摇瓶发酵培养,该重组菌的甘油产量为1g/L。渗透压胁迫测试证明该重组菌的耐渗透压性能较出发菌株有明显提高。     

试论汉语语素的类型及其相关概念

科学研究离不开分类 ,本文通过对汉语中最基本的语法单位语素的分类研究 ,不仅准确地定义了汉语虚词 ,而且理清了汉语语法研究中长期混淆不清的两个概念———汉语虚语与附加成分的界限