马纳斯河流域民主参与式的水资源统一管理

本文针对玛纳斯河流域水资源管理存在逐利、水资源被分割管理,以及缺乏民主参与等问题,提出流域实行水资源统一管理,成立利益相关者参与的民主管理监督机构。     

LabVIEW和三维虚拟现实技术在物理实验中的应用研究

虚拟仪器技术主要倾向于数据处理的精确性,三维虚拟技术则倾向于形象的仪器展示.利用两者的优势,我们创造性地实现了三维虚拟现实技术与虚拟仪器技术的完美结合,并以"电子示波器的使用"为例,探讨LabVIEW技术和3D技术在物理实验中的应用.     

Genetic deficiency of tartrate-resistant acid phosphatase associated with skeletal dysplasia, cerebral calcifications and autoimmunity

Vertebral and metaphyseal dysplasia, spasticity with cerebral calcifications, and strong predisposition to autoimmune diseases are the hallmarks of the genetic disorder spondyloenchondrodysplasia. We mapped a locus in five consanguineous families to chromosome 19p13 and identified mutations in ACP5, which encodes tartrate-resistant phosphatase (TRAP), in 14 affected individuals and showed that these mutations abolish enzyme function in the serum and cells of affected individuals. Phosphorylated osteopontin, a protein involved in bone reabsorption and in immune regulation, accumulates in serum, urine and cells cultured from TRAP-deficient individuals. Case-derived dendritic cells exhibit an altered cytokine profile and are more potent than matched control cells in stimulating allogeneic T cell proliferation in mixed lymphocyte reactions. These findings shed new light on the role of osteopontin and its regulation by TRAP in the pathogenesis of common autoimmune disorders.     

全桥变换器中磁通不平衡的抑制

分析了开关电源在采用全桥变换器的时候磁通不平衡现象的产生原因,并提出了实现抑制磁通不平衡的措施。着重介绍了应用阻隔电容实现磁通平衡的措施,同时进行了相关器件的设计与参数的计算。     

医用固体激光器脉冲式强预燃系统控制电路设计

根据激光医疗所需激光脉冲的特点,针对长脉冲Nd:YAG激光器泵浦闪光灯中脉冲式强预燃系统的控制电路展开研究,采用UCC3895芯片控制预燃电路工作状态的变化,提供所需的预燃电流,从而配合其他电路得到相应的激光脉冲。文中主要阐述以UCC3895为核心的脉冲式强预燃系统控制电路的工作原理,并且根据实验,最终验证了该套系统的可行性。     

XPDL与关系数据库之间的映射

XPDL( XML Process Definition Language)是工作流管理联盟(WFMC)制定的工作流模型标准,而关系数据库是当前很多工作流厂商存放工作流数据的首选方法.讨论了XPDL与关系数据库(RDB)之间的映射原理及映射方法,并由此建立了映射文件,从而方便了XPDL与RDB之间的双向数据转换.     

Science and technology for water purification in the coming decades

One of the most pervasive problems afflicting people throughout the world is inadequate access to clean water and sanitation. Problems with water are expected to grow worse in the coming decades, with water scarcity occurring globally, even in regions currently considered water-rich. Addressing these problems calls out for a tremendous amount of research to be conducted to identify robust new methods of purifying water at lower cost and with less energy, while at the same time minimizing the use of chemicals and impact on the environment. Here we highlight some of the science and technology being developed to improve the disinfection and decontamination of water, as well as efforts to increase water supplies through the safe re-use of wastewater and efficient desalination of sea and brackish water.     

Heterochromatin links to centromeric protection by recruiting shugoshin

The centromere of a chromosome is composed mainly of two domains, a kinetochore assembling core centromere and peri-centromeric heterochromatin regions. The crucial role of centromeric heterochromatin is still unknown, because even in simpler unicellular organisms such as the fission yeast Schizosaccharomyces pombe, the heterochromatin protein Swi6 (HP1 homologue) has several functions at centromeres, including silencing gene expression and recombination, enriching cohesin, promoting kinetochore assembly, and, ultimately, preventing erroneous microtubule attachment to the kinetochores. Here we show that the requirement of heterochromatin for mitotic chromosome segregation is largely replaced by forcibly enriching cohesin at centromeres in fission yeast. However, this enrichment of cohesin is not sufficient to replace the meiotic requirement for heterochromatin. We find that the heterochromatin protein Swi6 associates directly with meiosis-specific shugoshin Sgo1, a protector of cohesin at centromeres. A point mutation of Sgo1 (V242E), which abolishes the interaction with Swi6, impairs the centromeric localization and function of Sgo1. The forced centromeric localization of Sgo1 restores proper meiotic chromosome segregation in swi6 cells. We also show that the direct link between HP1 and shugoshin is conserved in human cells. Taken together, our findings suggest that the recruitment of shugoshin is the important primary role for centromeric heterochromatin in ensuring eukaryotic chromosome segregation.     

嵌入式数据库SQLite在家居能源控制子系统中的应用

针对家居能源控制系统测控点多,实时性和可靠性要求高的特点,提出了使用嵌入式数据库SQLite对系统的批量数据进行管理和维护.本文分别介绍了家居能源控制系统的总体设计,软件设计和数据库设计,以及在嵌入式系统WinCE4.2下SQLite的使用方法,并结合实例具体说明了SQLite在家居能源控制系统中的应用,最后用实验证明了SQLite的优越性.SQLite实现简单,可以提高程序的运行效率,增加程序的稳定性和可读性,因此具有广泛的应用前景.     

以MDR1基因启动子为靶点的药物筛选模型的建立

通过构建以MDR1启动子为启动序列的荧光素酶报告基因载体,建立基于双荧光素酶报告基因系统的多药耐药抑制剂筛选模型。从HCT-8细胞中提取DNA并克隆含有MDR1基因启动子的序列。将该序列重组到荧光素酶报告基因载体pGL-3-Basic的启动区域中,从而构建报告基因载体pGL-MDR1。将pGL-MDR1和pRL-TK载体共转染到HCT-8和HCT-8/VCR细胞中,建立用于筛选多药耐药抑制剂的方法。通过调节不同载体的比例来优化转染效率。通过MDR1基因激活剂（热诱导）和抑制剂（EGCG）来验证该方法。通过直接测序法验证了pGL-MDR1含有MDR1基因启动子序列且没有出现碱基突变。在n（pGL-MDR1）∶n（pRL-TK）=5∶5时,转染效率最高并具有最高的荧光素酶活性。通过MDR1基因激活处理后表现为时间依赖性地激活MDR1基因的表达,而MDR1基因抑制剂的作用则相反。