(−)-Epigallocatechin gallate,a polyphenolic tea antioxidant,inhibits peroxynitritemediated formation of 8-oxodeoxyguanosine and 3-nitrotyrosine

Reaction with peroxynitrite at pH 7.4 and 37°C was found to increase the 8-oxodeoxyguanosine levels in calf thymus DNA 35-38-fold. This oxidation of deoxyguanosine, as well as the peroxynitrite-mediated nitration of tyrosine to 3-nitrotyrosine, was significantly inhibited by ascorbic acid, glutathione and (–)-epigallocatechin gallate, a polyphenolic antioxidant present in tea. For 50% inhibition of the oxidation of deoxyguanosine to 8-oxodeoxyguanosine, 1.1, 7.6 of 0.25 mM ascorbate, glutathione or (–)-epigallocatechin gallate, respectively, was required. For 50% inhibition of tyrosine nitration, the respective concentrations were 1.4, 4.6 or 0.11 mM. Thus, (–)-epigallocatechin gallate is a significantly better inhibitor of both reactions than either ascorbate or glutathione. Reaction of (–)-epigallocatechin gallate with peroxynitrite alone resulted in the formation of a number of products. Ultraviolet spectra of two of these suggest that the tea polyphenol and/or its oxidation products are nitrated by peroxynitrite.     

饱和脂肪烃一元取代物（C_nH_（2n＋1）Y）异构体总数的计算机求解方法

本文介绍了在微机上用ＰＡＳＣＡＬ语言计算饱和脂肪烃一元取代物的伯、仲、叔异构体数目及总数目的方法。为实现递归计算，突破无动态数组的编程障碍，程序使用查询计算方法，并采用ＰＡＳＣＡＬ语言文件系统存放中间数据和最后结果。计算数据准确可靠。     

库车冬小麦品种演替规律调查分析

对库车县建国以来历年种植的冬小麦品种的来源、引进目的、种植年限、产量表现、推广面积、抗病虫能力、增产潜力、生态特点和淘汰原因等进行了调查，从而为找出该地区最适于种植的高产、优质品种提供依据。     

Growth of Magnetite Nanoparticles under Magnetic Fields

1 Introduction Over the past several years, the preparation and characterization ofnanoscale magnetic materials, especially one-dimensional (1D) nanostructure, have attracted much attention as the nanomaterials would allow investigating the fundamental aspects of magnetic-ordering phenomena in magnetic materials with reduced dimensions and could lead to new potential applications such as data storage technology[1-6].     

Java相关应用技术的兼容性测试

首先介绍了一些兼容性测试的概念,包括概念的引入,兼容性测试的特征及其优越性,随后具体介绍了Sun公司的TCK测试工具,阐述了TCK工具的工作原理,工作界面,结果的处理等情况。     

略论唐朝治桂政策实施的背景

唐朝以前，桂东地区的封建庄园经济。土地私有制已有所发展，而桂西地区大体上仍停留在前封建制阶段，社会经济相当落后，尤其是农业经济与桂东有着相当大的差距。唐朝统治者审时度势，根据当时广西社会发展的变化，调整了其治桂政策，在桂东地区设立正州正县，而在桂西地区则继续沿袭前代的的羁縻政策。     

粘果山羊草Aegilops kotschyi puroindoline a基因的研究

根据已报道的小麦籽粒硬度主效基因之一puroindoline a（Pina）基因的保守序列,设计并合成了一对特异性引物ForA1和RevA1,对粘果山羊草Aegilops kotschyi的三个材料的基因组DNA和互补DNA进行Pina基因克隆、序列测定和表达分析,发现了三个新型Pina突变体,基因序列与六倍体小麦的同源基因存在较大的差异.RT-PCR证实了Pina基因在籽粒胚乳中表达.Southern blot分析结果表明,三种材料中该基因均含有两个拷贝.研究结果显示山羊草是一个丰富的遗传资源库,为小麦籽粒硬度的基因工程改良奠定了基础.     

Minimum information about a microarray experiment (MIAME)-toward standards for microarray data.

Microarray analysis has become a widely used tool for the generation of gene expression data on a genomic scale. Although many significant results have been derived from microarray studies, one limitation has been the lack of standards for presenting and exchanging such data. Here we present a proposal, the Minimum Information About a Microarray Experiment (MIAME), that describes the minimum information required to ensure that microarray data can be easily interpreted and that results derived from its analysis can be independently verified. The ultimate goal of this work is to establish a standard for recording and reporting microarray-based gene expression data, which will in turn facilitate the establishment of databases and public repositories and enable the development of data analysis tools. With respect to MIAME, we concentrate on defining the content and structure of the necessary information rather than the technical format for capturing it.     

Mitochondrial dysfunction and apoptosis in myopathic mice with collagen VI deficiency

Collagen VI is an extracellular matrix protein that forms a microfilamentous network in skeletal muscles and other organs. Inherited mutations in genes encoding collagen VI in humans cause two muscle diseases, Bethlem myopathy and Ullrich congenital muscular dystrophy. We previously generated collagen VI-deficient (Col6a1-/-) mice and showed that they have a muscle phenotype that strongly resembles Bethlem myopathy. The pathophysiological defects and mechanisms leading to the myopathic disorder were not known. Here we show that Col6a1-/- muscles have a loss of contractile strength associated with ultrastructural alterations of sarcoplasmic reticulum (SR) and mitochondria and spontaneous apoptosis. We found a latent mitochondrial dysfunction in myofibers of Col6a1-/- mice on incubation with the selective F1F(O)-ATPase inhibitor oligomycin, which caused mitochondrial depolarization, Ca2+ deregulation and increased apoptosis. These defects were reversible, as they could be normalized by plating Col6a1-/- myofibers on collagen VI or by addition of cyclosporin A (CsA), the inhibitor of mitochondrial permeability transition pore (PTP). Treatment of Col6a1-/- mice with CsA rescued the muscle ultrastructural defects and markedly decreased the number of apoptotic nuclei in vivo. These findings indicate that collagen VI myopathies have an unexpected mitochondrial pathogenesis that could be exploited for therapeutic intervention.