Cytochemically rich neuroglia invest metacercariae in the brain ofPhoxinus phoxinus L.

Summary Histochemical determination of neuroglia investing parasites in naturally infected fish depends upon recognition of the highly soluble or diffusible nature of the cytoplasm.Acknowledgment. M.R.L. Johnston advised me during the course of this work.     

Dystrophic mutation (dy2J) affecting regulation of lactate dehydrogenase (LDH) and pyruvate kinase (PK) in C57BL/6J mice

Summary The genotype difference (dystrophic vs nondystrophic) in the LDH isozymes is observed in kidney. These differences are evident only at birth and at early developmental stages (before the expression of dystrophic symptoms). The tissue specific genotype differences for PK are limited to the thigh muscle (M form) and heart (L form), after the onset of the condition. These differences may reflect the pleiotropic effect of the dy^2J locus during the temporal regulation of these and other enzymes implicated in muscular dystrophy (MD).This research was financed by a Natural Sciences and Engineering Research Council Canada grant to S.M.S.     

Development of an enzyme linked immunosorbent assay (ELISA) for measurement of vitellogenin concentrations in cockroaches

Summary A competitive enzyme linked immunosorbent assay was developed for the quantification of a large lipoprotein, namely the yolk protein vitellogenin, in the haemolymph of cockroaches (Nauphoeta cinerea). This assay was found to be specific, reproducible and it has a high sensitivity (approximately 10 ng).Acknowledgments. We would like to express our thanks to Prof. H. Fey and Ms H. Pfister, Veterinary Bacteriological Institute, University of Berne, for introducing us into the ELISA methodology, to P. Beyeler for technical assistance and to M. Kaltenrieder for drawing the graphs. We also thank the Sandoz Foundation for a fellowship to M. Dumas and the Swiss National Science Foundation (grant No. 3. 188-0.77) for financial support.     

Inhibition of neurotensin (NT)-induced glucagon release by [D-Trp11]-NT1

Summary A synthetic analog of neurotensin (NT), [D-Trp¹¹]-NT, antagonized NT-induced hyperglucagonemia and hyperglycemia, and also NT-induced glucagon release from pancreatic islets in rats.This work was supported in part by a grant from the Imanaga Medical Research Foundation, Nagoya, Japan. Address for reprint requests: M.U., Department of Surgery, National Nagoya Railway Hospital, Taiko 1-chome, Nakamura-ku, Nagoya 453, Japan.