排序方式: 共有49条查询结果,搜索用时 15 毫秒
21.
Ricci-Vitiani L Lombardi DG Pilozzi E Biffoni M Todaro M Peschle C De Maria R 《Nature》2007,445(7123):111-115
Colon carcinoma is the second most common cause of death from cancer. The isolation and characterization of tumorigenic colon cancer cells may help to devise novel diagnostic and therapeutic procedures. Although there is increasing evidence that a rare population of undifferentiated cells is responsible for tumour formation and maintenance, this has not been explored for colorectal cancer. Here, we show that tumorigenic cells in colon cancer are included in the high-density CD133+ population, which accounts for about 2.5% of the tumour cells. Subcutaneous injection of colon cancer CD133+ cells readily reproduced the original tumour in immunodeficient mice, whereas CD133- cells did not form tumours. Such tumours were serially transplanted for several generations, in each of which we observed progressively faster tumour growth without significant phenotypic alterations. Unlike CD133- cells, CD133+ colon cancer cells grew exponentially for more than one year in vitro as undifferentiated tumour spheres in serum-free medium, maintaining the ability to engraft and reproduce the same morphological and antigenic pattern of the original tumour. We conclude that colorectal cancer is created and propagated by a small number of undifferentiated tumorigenic CD133+ cells, which should therefore be the target of future therapies. 相似文献
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Lucia Wiest 《Cellular and molecular life sciences : CMLS》1969,25(8):848-848
Résumé Après l'hypophysectomie, chez les rats Wistar mâles, le nombre des cellules mononucléaires tetraploÏdes du foie s'abaisse de 70% à 28%, tandis que le nombre des octoploÏdes diminue de 19,8% à 0%. 相似文献
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Schmutz J Wheeler J Grimwood J Dickson M Yang J Caoile C Bajorek E Black S Chan YM Denys M Escobar J Flowers D Fotopulos D Garcia C Gomez M Gonzales E Haydu L Lopez F Ramirez L Retterer J Rodriguez A Rogers S Salazar A Tsai M Myers RM 《Nature》2004,429(6990):365-368
As the final sequencing of the human genome has now been completed, we present the results of the largest examination of the quality of the finished DNA sequence. The completed study covers the major contributing sequencing centres and is based on a rigorous combination of laboratory experiments and computational analysis. 相似文献
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A recurrent 15q13.3 microdeletion syndrome associated with mental retardation and seizures 总被引:1,自引:0,他引:1
Sharp AJ Mefford HC Li K Baker C Skinner C Stevenson RE Schroer RJ Novara F De Gregori M Ciccone R Broomer A Casuga I Wang Y Xiao C Barbacioru C Gimelli G Bernardina BD Torniero C Giorda R Regan R Murday V Mansour S Fichera M Castiglia L Failla P Ventura M Jiang Z Cooper GM Knight SJ Romano C Zuffardi O Chen C Schwartz CE Eichler EE 《Nature genetics》2008,40(3):322-328
We report a recurrent microdeletion syndrome causing mental retardation, epilepsy and variable facial and digital dysmorphisms. We describe nine affected individuals, including six probands: two with de novo deletions, two who inherited the deletion from an affected parent and two with unknown inheritance. The proximal breakpoint of the largest deletion is contiguous with breakpoint 3 (BP3) of the Prader-Willi and Angelman syndrome region, extending 3.95 Mb distally to BP5. A smaller 1.5-Mb deletion has a proximal breakpoint within the larger deletion (BP4) and shares the same distal BP5. This recurrent 1.5-Mb deletion contains six genes, including a candidate gene for epilepsy (CHRNA7) that is probably responsible for the observed seizure phenotype. The BP4-BP5 region undergoes frequent inversion, suggesting a possible link between this inversion polymorphism and recurrent deletion. The frequency of these microdeletions in mental retardation cases is approximately 0.3% (6/2,082 tested), a prevalence comparable to that of Williams, Angelman and Prader-Willi syndromes. 相似文献
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Lucia Banci Ivano Bertini Francesca Cantini Simone Ciofi-Baffoni 《Cellular and molecular life sciences : CMLS》2010,67(15):2563-2589
Copper is an essential but potentially harmful trace element required in many enzymatic processes involving redox chemistry.
Cellular copper homeostasis in mammals is predominantly maintained by regulating copper transport through the copper import
CTR proteins and the copper exporters ATP7A and ATP7B. Once copper is imported into the cell, several pathways involving a
number of copper proteins are responsible for trafficking it specifically where it is required for cellular life, thus avoiding
the release of harmful free copper ions. In this study we review recent progress made in understanding the molecular mechanisms
of copper transport in cells by analyzing structural features of copper proteins, their mode of interaction, and their thermodynamic
and kinetic parameters, thus contributing to systems biology of copper within the cell. 相似文献
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Tommy Baumann Lucia Kuhn-Nentwig Carlo R. Largiadèr Wolfgang Nentwig 《Cellular and molecular life sciences : CMLS》2010,67(15):2643-2651
Defensins are a major family of antimicrobial peptides found throughout the phylogenetic tree. From the spider species: Cupiennius salei, Phoneutria reidyi, Polybetes pythagoricus, Tegenaria atrica, and Meta menardi, defensins belonging to the ‘ancestral’ class of invertebrate defensins were cloned and sequenced. The deduced amino acid
sequences contain the characteristic six cysteines of this class of defensins and reveal precursors of 60 or 61 amino acid
residues. The mature peptides consist of 37 amino acid residues, showing up to 70% identities with tick and scorpion defensins.
In C. salei, defensin mRNA was found to be constitutively expressed in hemocytes, ovaries, subesophageal nerve mass, hepatopancreas,
and muscle tissue. This is the first report presenting and comparing antimicrobial peptides belonging to the family of defensins
from spiders. 相似文献
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Isolation of an E. coli strain with a mutation affecting DNA polymerase 总被引:165,自引:0,他引:165
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Inherent toxicity of aggregates implies a common mechanism for protein misfolding diseases 总被引:68,自引:0,他引:68
Bucciantini M Giannoni E Chiti F Baroni F Formigli L Zurdo J Taddei N Ramponi G Dobson CM Stefani M 《Nature》2002,416(6880):507-511
A range of human degenerative conditions, including Alzheimer's disease, light-chain amyloidosis and the spongiform encephalopathies, is associated with the deposition in tissue of proteinaceous aggregates known as amyloid fibrils or plaques. It has been shown previously that fibrillar aggregates that are closely similar to those associated with clinical amyloidoses can be formed in vitro from proteins not connected with these diseases, including the SH3 domain from bovine phosphatidyl-inositol-3'-kinase and the amino-terminal domain of the Escherichia coli HypF protein. Here we show that species formed early in the aggregation of these non-disease-associated proteins can be inherently highly cytotoxic. This finding provides added evidence that avoidance of protein aggregation is crucial for the preservation of biological function and suggests common features in the origins of this family of protein deposition diseases. 相似文献