Natural selection has driven population differentiation in modern humans

The considerable range of observed phenotypic variation in human populations may reflect, in part, distinctive processes of natural selection and adaptation to variable environmental conditions. Although recent genome-wide studies have identified candidate regions under selection, it is not yet clear how natural selection has shaped population differentiation. Here, we have analyzed the degree of population differentiation at 2.8 million Phase II HapMap single-nucleotide polymorphisms. We find that negative selection has globally reduced population differentiation at amino acid-altering mutations, particularly in disease-related genes. Conversely, positive selection has ensured the regional adaptation of human populations by increasing population differentiation in gene regions, primarily at nonsynonymous and 5'-UTR variants. Our analyses identify a fraction of loci that have contributed, and probably still contribute, to the morphological and disease-related phenotypic diversity of current human populations.     

Distribution patterns of mammalian-like peptide immunoreactive cells in the midgut ofAeshna cyanea (Insecta,Odonata)

Summary The distribution of cells reacting with antisera to cholecystokinin, substance P, gonadoliberin, methionine-enkephalin, and vasoactive intestinal peptide, demonstrated by the indirect immunoperoxidase method, was studied along the entire midgut of an insect,Aeshna cyanea. For each antiserum, the number of reacting cells increased from the middle part to the end of the midgut. Only a few cells reacted to somatoliberin, leucin-enkephalin and somatostatin antisera. In the connective sheath surrounding the midgut epithelium, nerve fibers were stained by antisera to serotonin, somatostatin, cholecystokinin, vasoactive intestinal peptide and methionine-enkephalin.     

The structure and function of Escherichia coli penicillin-binding protein 3

Escherichia coli penicillin-binding protein PBP3 is a key element in cell septation. It is presumed to catalyse a transpeptidation reaction during biosynthesis of the septum peptidoglycan but, in vitro, its enzymatic activity has only been demonstrated with thiolester analogues of the natural peptide substrate. It has no detectable transglycosylase activity with lipid II as substrate. This tripartite protein is constructed of an N-terminal membrane anchor-containing module that is essential for cell septation, a non-penicillin-binding (n-PB) module of unknown function and a C-terminal penicillin-binding (PB) module exhibiting all the characteristic motifs of penicilloyl serine transferases. The n-PB module, which is required for the folding and stability of the PB module, may provide recognition sites for other cell division proteins. Initiation of septum formation is not PBP3-dependent but rests on the appearance of the FtsZ ring, and is thus penicillin-insensitive. The control of PBP3 activity during the cell cycle is briefly discussed.     

Casein, a prohormone with an immunomodulating role for the newborn?

Maternal colostrum and milk, the earliest food of the newborn, should not only be considered as supplying nutrients, but also as agents providing protection against aggressions from the new environment. Indeed by enzymatic digestion of the main milk proteins, the caseins, biologically active peptides are released; they may be implicated in the stimulation of the newborn's immune system. From this point of view a 'strategic active zone' has been characterized in beta-casein. A possible role of casein as a 'prohormone' for the newborn is suggested.     

Radioprotective effects of the immunostimulating lauroylpeptide LtriP (RP 56142)

The lipopeptide lauroyl-L-Ala--D-Glu-L,L-A₂pm (LtriP) increased the resistance of mice to the lethal effect of -ray irradiation. The radioprotective effect was dependent on the doses of LtriP and of radiation. Maximum survival was observed when the lipopeptide was injected on two successive days before irradiation. This activity seems to be related to immunostimulating functions, since the non-immunostimulating analog lauroyl-L-Ala--D-Glu-D,D-A₂pm-Gly, containing D,D-diaminopimelic acid, was not radioprotective. The protective activity might result from an induction of cytokines, such as IL-1, TNF and M-CSF, since LtriP induced the mRNA expression and the secretion of these immunomodulators.     

Influence of phenylbutazone on bile flow in the rat

Summary Phenylbutazone, a well-known enzyme inducer, at a dose of 80 mg·kg^–1 once daily for 8 days increases liver weight and bile flow expressed per g of liver (p<0.01). The bile salt secretory rate is not increased.     

Anti-feedant activity of the polyacetylene,phenylheptatriyne (PHT), from the Asteraceae toEuxoa messoria (Lepidoptera: Noctuidae)

Summary Phenylheptatriyne (PHT), a polyacetylene from various species of Asteraceae reduced feeding and weight gain of larvae of the polyphagous insectEuxoa messoria when incorporated into an artificial diet at concentrations of 10–300 ppm. These results suggest a role as insect antifeedants for the widely distributed polyacetylenes of the Asteraceae.Acknowledgment. This work was supported by NSERC and Agriculture Canada (E.M.R.) We thank Dr R. J. Byers (Agriculture Canada) for eggs ofEuxoa. To whom reprint requests should be addressed.     

Chlorpyrifos (Dursban®) resistance inCulex pipiens pipiens L. from Southern France: Inheritance and linkage

Summary InCulex pipiens mosquitos from Southern France, resistance to the organophosphorus insecticide chlorpyrifos is due to the dominant allele (Chl ^R) of an autosomal gene. TheChl gene is localized between thea-Gpd andEst-2 loci at 26.8 and 5.8 units of crossing-over respectively.     

Are anticholinergic effects responsible for the heterogeneous quinidine-induced modifications of heart muscle refractoriness?

Cellular and Molecular Life Sciences - Vagal tone is responsible for the heterogeneous reactivity of atrial and ventricular contractile tissues to quinidine. Acetylcholine may make atrial cells...