Withdrawing Unfalsifiable Hypotheses

There has been little research into the weak kindsof negating hypotheses. Hypotheses may be unfalsifiable. In this case it is impossible tofind a contradiction in some area of the conceptualsystems in which they are incorporated.Notwithstanding this fact, it is sometimes necessaryto construct ways of rejecting the unfalsifiablehypothesis at hand by resorting to some external forms of negation, external because wewant to avoid any arbitrary and subjectiveelimination, which would be rationally orepistemologically unjustified. I will consider akind of ``weak' (unfalsifiable) hypotheses that arehard to negate and the ways for making it easy. Inthese cases the subject can ``rationally' decide towithdraw his hypotheses even in contexts where it is``impossible' to find ``explicit' contradictions: theuse of negation as failure (an interestingtechnique for negating hypotheses and accessing newones suggested by artificial intelligence) isilluminating. I plan to explore whether this kind ofnegation can be employed to model hypothesiswithdrawal in Poincaré's conventionalism of theprinciples of physics and in Freudian analyticreasoning.     

Stimulated platelets use serotonin to enhance their retention of procoagulant proteins on the cell surface.

Activated platelets bind numerous adhesive and procoagulant proteins by receptor-mediated processes. Although there is little evidence to suggest that these processes are heterogeneous in platelets, we previously found that platelets co-stimulated with collagen and thrombin express functional alpha-granule factor V only on a subpopulation of cells. Here we show that these cells, referred to as 'COAT-platelets', bind additional alpha-granule proteins, including fibrinogen, von Willebrand factor, thrombospondin, fibronectin and alpha2-antiplasmin. These proteins are all transglutaminase substrates, and inhibitors of transglutaminase prevent the production of COAT-platelets. A synthetic transglutaminase substrate (CP15) also binds to COAT-platelets, and analysis by high performance liquid chromatography/mass spectrometry shows that a product is formed with a relative molecular mass (Mr) equal to CP15 plus 176. Serotonin, an abundant component of platelet-dense granules, has an Mr of 176, and fibrinogen isolated from COAT-platelets contains covalently linked serotonin. Synthetic bovine serum albumin-(serotonin)6 binds selectively to COAT-platelets and also inhibits the retention of procoagulant proteins on COAT-platelets. These data indicate that COAT-platelets use serotonin conjugation to augment the retention of procoagulant proteins on their cell surface through an as yet unidentified serotonin receptor.     

Karyotypes of four Artemisia species: A. carruthii, A. filifolia, A. Frigida, and A. spinescens

Artemisia carruthii and A. frigida of the subgenus Artemisia and A. filifolia and A. spinescens of the subgenus Dracunculus all have chromosome numbers based on x = 9. Diploid (2n = 18) karyotypes of each species are composed of large, medium, and small chromosomes that are mainly metacentric and submetacentric. The individual karyotypes are similar but distinctive. Artemisia filifolia ’s karyotype and chemistry is quite similar to that of Section Tridentatae, but A. filifolia has significant morphological differences with respect to the Tridentatae. Artemisia spinescens includes a tetraploid (2n = 36) population as well as diploid populations. Karyotypic analysis of a tetraploid A. spinescens suggests that it is an autotetraploid, thus carrying out a common theme in Artemisia (autopolyploidy).     

Directional postcopulatory sexual selection revealed by artificial insemination

Postcopulatory sexual selection comprises both sperm competition, where the sperm from different males compete for fertilization, and cryptic female choice, where females bias sperm use in favour of particular males. Despite intense current interest in both processes as potential agents of directional sexual selection, few studies have attributed the success of attractive males to events that occur exclusively after insemination. This is because the interactions between pre- and post-insemination episodes of sexual selection can be important sources of variation in paternity. The use of artificial insemination overcomes this difficulty because it controls for variation in male fertilization success attributable to the female's perception of male quality, as well as effects due to mating order and the relative contribution of sperm from competing males. Here, we adopt this technique and show that in guppies, when equal numbers of sperm from two males compete for fertilization, relatively colourful individuals achieve greater parentage than their less ornamented counterparts. This finding indicates that precopulatory female mating preferences can be reinforced exclusively through postcopulatory processes occurring at a physiological level. Our analysis also revealed that relatively small individuals were advantaged in sperm competition, suggesting a possible trade-off between sperm competitive ability and body growth.     

Biographical Notes

Note

Biographical Notes     

Extended surface chirality from supramolecular assemblies of adsorbed chiral molecules

The increasing demand of the chemical and pharmaceutical industries for enantiomerically pure compounds has spurred the development of a range of so-called 'chiral technologies' (ref. 1), which aim to exert the ultimate control over a chemical reaction by directing its enantioselectivity. Heterogeneous enantioselective catalysis is particularly attractive because it allows the production and ready separation of large quantities of chiral product while using only small quantities of catalyst. Heterogeneous enantioselectivity is usually induced by adsorbing chiral molecules onto catalytically active surfaces. A mimic of one such catalyst is formed by adsorbing (R,R)-tartaric acid molecules on Cu(110) surfaces: this generates a variety of surface phases, of which only one is potentially catalytically active, and leaves the question of how adsorbed chiral molecules give rise to enantioselectivity. Here we show that the active phase consists of extended supramolecular assemblies of adsorbed (R,R)-tartaric acid, which destroy existing symmetry elements of the underlying metal and directly bestow chirality to the modified surface. The adsorbed assemblies create chiral 'channels' exposing bare metal atoms, and it is these chiral spaces that we believe to be responsible for imparting enantioselectivity, by forcing the orientation of reactant molecules docking onto catalytically active metal sites. Our findings demonstrate that it is possible to sustain a single chiral domain across an extended surface--provided that reflection domains of opposite handedness are removed by a rigid and chiral local adsorption geometry, and that inequivalent rotation domains are removed by successful matching of the rotational symmetry of the adsorbed molecule with that of the underlying metal surface.     

Chromosomal relationships among three species of jackrabbits ( Lepus : Leporidae) from Mexico

We studied differentially stained karyotypes of the leporids Lepus californicus, L. callotis, and L. flavigularis and described their banding patterns of euchromatin to decide whether results fit expectations of chromosomal rearrangements leading to the karyotypic evolution ancestral of the L. californicus--L. callotis--L. flavigularis lineage. Results confirmed previous findings on numbers of chromosomes (2 n 's) and fundamental numbers (FNs; except for L. californicus ). Homologous G-banding patterns allowed identification of chromosome rearrangements such as pericentric inversions and addition and deletion of euchromatin. Chromosomal rearrangements that we deduced mostly support hypotheses on karyotypic changes in the chromosomal evolution of the Lepus ancestral lineage that we examined. The karyotypic pattern coincides with patterns of geographic variation and distribution of the species examined.     

Isoform specific phosphorylation of p53 by protein kinase CK1

The ability of three isoforms of protein kinase CK1 (α, γ₁, and δ) to phosphorylate the N-terminal region of p53 has been assessed using either recombinant p53 or a synthetic peptide reproducing its 1–28 sequence. Both substrates are readily phosphoylated by CK1δ and CK1α, but not by the γ isoform. Affinity of full size p53 for CK1 is 3 orders of magnitude higher than that of its N-terminal peptide (K _m 0.82 μM vs 1.51 mM). The preferred target is S20, whose phosphorylation critically relies on E17, while S6 is unaffected despite displaying the same consensus (E-x-x-S). Our data support the concept that non-primed phosphorylation of p53 by CK1 is an isoform-specific reaction preferentially affecting S20 by a mechanism which is grounded both on a local consensus and on a remote docking site mapped to the K²²¹RQK²²⁴ loop according to modeling and mutational analysis.