Mapping CD20 molecules on the lymphoma cell surface using atomic force microscopy

Atomic force microscopy (AFM) was used to locate CD20 molecules on the surface of lymphoma Raji cells. Rituximab (a monoclonal antibody against CD20) molecules were linked onto the AFM tip via a polyethylene glycol (PEG) linker. Raji cells were adsorbed onto glass slides coated with poly-L-lysine. First, the CD20 distribution in a local area of the cell surface was visualized using the AFM lift scan mode. Second, 16 × 16 force curves were obtained from the same cell area to construct the CD20-rituximab binding force map. Finally, free rituximab was added to block the CD20 molecules on the cell surface and the lift phase image and CD20-rituximab force map were obtained again. The experimental results indicated that when the lift height was greater than the length of the PEG linker, no recognition sites were observed in the lift phase image. However, as the lift height decreased to the length of the PEG linker, some recognition sites were observed in the lift phase image and these sites were generally consistent with the pixels in the force map. After blocking, both the recognition sites in the lift phase image and the gray pixels in the binding force map decreased markedly. These results can improve our understanding of the distribution of protein molecules on the cell surface and facilitate further investigations into cellular functions.     

浅析元白判文

唐代中期，元白的判文盛行一时，他们的判文在内容和风格上极为相似。并被广大学子引为判文之圭臬，竞相仿作。究其原因，无非是时代的因素和元白判文的精妙所造就。文章从元白判文的内容和艺术特色等方面进行了研究，从而揭示了元白判文盛行的原因所在。     

无意识笼罩下的俄狄浦斯情结——《雷雨》中男性人物的心理分析

用精神分析中的无意识方法分析了《雷雨》中三个男性人物的行为和心理，从中审视作者的创作心理，揭示了二者的内在联系。     

山西省黄土风情旅游研究

文章利用层次分析法这一定性和定量相结合的方法对山西省黄土风情旅游进行了分析,得出山西黄土风情旅游资源品质高,特征独特文化底蕴深厚,奇特度、美感度较高,但山西的旅游环境和交通设施以及人员素质都需提高的结论.文章在此基础上提出了对策,为山西旅游业可持续发展提供了思路和依据.     

科学构建高等教育体系的思考

大众化高等教育迅猛发展,一举打破原有的精英教育格局,而适应大众化高等教育发展阶段的新的高等教育体系尚未建立起来,这必然制约大众化高等教育进一步健康发展.因此,科学构建高等教育体系成为迫切需要解决的问题.通过高等教育的本质分析,同时兼顾高等教育的价值和功能为分类依据,提出将高等教育体系划分为学术性高等教育、应用性高等教育、成人继续教育.     

论陈寅恪对中国比较文学学科发展的贡献

从学科自觉的角度看，陈寅恪先生对比较文学学科发展的贡献主要体现在三个方面：第一，他是中国第一位从学科自觉高度提出比较文学定义的学者；第二，他用理论与实践活动丰富与发展了比较文学的研究类型、范式与研究方法；第三，他在跨文化传播与接受方面提出了自己一系列独到的见解。     

通信电子战信号反演处理——I：概论

通信电子战信号处理迫切需要一个具有宏观指导意义的方法论。反演方法既是地球物理学中的基本处理方法，又是人类两大基本推理方法的一个子方法。讨论了反演方法及其理论的一般定义。指出在电子学与通信及其信号处理领域，将问题统一到信号反演的框架下，不仅有利于对处理方法的理论研究，而且有利于吸收那些在非电子学领域已经建立的反演方法及其理论。给出了关于通信电子战信号反演处理的定义，并提出其概念模型和目标参数。     

Effect of intermittent aeration on the treatment performance in a submerged membrane bioreactor

In order to improve removal for nitrogen in a pilot-scale submerged membrane bioreactor (SMBR), intermittent aeration was conducted, and the effect on the treatment performance under four kinds of operation condition (run 1, continuous aeration; run 2, 60/60 min aeration on/off time; run 3, 60/90 min aeration on/off time; run 4, 60/75 min aeration on/off time) was evaluated. The results showed that depending on the specific on/off of the aeration time ratio, removal efficiency of nitrogen could be improved significantly, and the removal rates of total nitrogen (TN) under different operation conditions were 28.0%, 59.5%, 66.8% and 70.7%, respectively. There were no obvious differences for removal rates for COD_Cr and ammonia among different operation conditions. In general, intermittent aeration could be used as a feasible way to improve treatment performance for nitrogen in the SMBR.     

隧道地质预报技术和方法

隧道地质超前预报是防止隧道开挖突发性灾害、保证施工工期、及时调整支护参数、保证施工质量和控制隧道建设成本的有效手段。在施工阶段采用短时间对岩体进行物理探测,通过各种信息来判断岩体特征的物理探测方法。     

Research on Deformation Regularity of Surface Migrating With Waste-fill Mining

Using FLAC3D numerical simulation software,the influence laws that the different mining depth,mining width and filling step have on the surface subsidence and horizontal movement under the condition of filling mining has been studied.The functional relation among full subsidence and horizontal movement of surface,mining depth,mining width and filling step has been given,which provides theoretical formulas been referenced for filling mining theory and has a certain guiding value.