Physiological studies on the effects of nutritional imbalance on the central nervous system. II. Effects of thiamine deficiency on oxidative enzymes in the brain of chicken, Gallus domesticus.

The activitiy levels of succinate dehydrogenase, glutamate dehydrogenase and pyruvate dehydrogenase in the fore, mid and hind brain regions of the thiamine deficient chicken, Gallus domesticus were determined. The activity levels of succinate dehydrogenase and glutamate dehydrogenase in all the 3 regions of brain showed augmentation on inducing thiamine deficiency. In contrast the activity levels of pyruvate dehydrogenase decreased in the brain of thiamine deficient animals. It is suggested that these changes in the oxidative enzymes indicate disturbance caused in the operation of the tricarboxylic acid cycle in thiamine deficiency.     

Increase of interferon antiviral activity by exogenous cyclic adenosine-3':5'-monophosphate (cAMP).

Some effects of cAMP on replication of Semliki Forest Virus in chick embryo fibroblast cell cultures are described. Depending on concentration, the incorporation of [3H]-uridine into viral RNA or the formation of plaque-forming units is inhibited; the highest concentration tested was 8 mM. Cyclic AMP has an effect of its own and increases the Interferon action in the lower concentration ranges of Interferon (up to 1 unit/ml). The effect of cyclic AMP is fast, needs no induction and is also visible in late phases of viral replication. However, these experiments do not establish a causal relation between cAMP and Interferon.     

用变系数微分方程进行船舶波浪弯矩的时域模拟

对船舶在波浪上的刚体运动理论加以改进，采用变系数微分方程对船舶在波浪上的运动进行模拟，并计算外力，计算表明，船舶在正弦波上的运动是一种近似于“拍”的运动，不是通常理论所假设的正弦周期运动，在微机上开发的程序能模拟各种规则波、非规则波输入时的船舶运动情况，为船舶结构可靠性分析中的外力随机分析奠定了基础。算例与文献进行了比较，表明了其正确性。     

Intraclonal generation of antibody mutants in germinal centres

The generation and selection of somatic antibody mutants are key elements of acquired immunity, essential for the affinity maturation of antibody responses dependent on T cells. The mutants are generated through a mechanism that introduces point mutations at high rate into rearranged variable (V) region genes in the course of cell proliferation. Their appearance coincides with the generation of germinal centres, which are characterized by oligoclonal B-cell proliferation and have been suggested to be the microenvironment in which antibody mutants are generated. We report here direct evidence for this hypothesis. Rearranged V-region genes were amplified from the genomic DNA of cells picked from individual germinal centres. The sequence analysis of these genes revealed that most represent cells of distinct B-cell clones which expanded locally, generating somatic antibody mutants at high rate. By contrast, antigen-induced proliferation of B cells at another site, periarteriolar lymphocyte sheath-associated foci, was not associated with somatic hypermutation.     

一种评价自动目标检测算法性能的方法

提出一种自动目标检测算法性能的评价方法，设计了一种实景目标背景图像合成方法，由此可以得到满足测试目标检测算法性能的要求且度量参数调整方便的测试图像集，确定了１６个图像质量度量指标，建立了性能评价的响应函数模型，采用因子分析、多元相关回归分析和Ｌｏｇｉｓｔｉｃ回归分析方法，研究算法性能与图像度量的统计关系，实验表明，所提出的目标检测算法性能评价方法是合理的。     

Regulation of p34CDC28 tyrosine phosphorylation is not required for entry into mitosis in S. cerevisiae.

Progression from G2 to M phase in eukaryotes requires activation of a protein kinase composed of p34cdc2/CDC28 associated with G1-specific cyclins. In some organisms the activation of the kinase at the G2/M boundary is due to dephosphorylation of a highly conserved tyrosine residue at position 15 (Y15) of the cdc2 protein. Here we report that in the budding yeast Saccharomyces cerevisiae, p34CDC28 also undergoes cell-cycle regulated dephosphorylation on an equivalent tyrosine residue (Y19). However, in contrast to previous observations in S. pombe, Xenopus and mammalian cells, dephosphorylation of Y19 is not required for the activation of the CDC28/cyclin kinase. Furthermore, mutation of this tyrosine residue does not affect dependence of mitosis on DNA synthesis nor does it abolish G2 arrest induced by DNA damage. Our data imply that regulated phosphorylation of this tyrosine residue is not the 'universal' means by which the onset of mitosis is determined. We propose that there are other unidentified controls that regulate entry into mitosis.     

FMS建模和形式化验证

提出了一种FMS的建模方法,此方法是基于时间自动机模型。运用模型检查工具UPPAAL来进行建模,仿真和验证。由于时间的因素被考虑到建模方法中,因此FMS的调度和控制问题可以被集成到这个模型中。在系统的行为被控制的同时,可以保证系统的性能指标。从而最优调度和最优控制可以得到检查和验证。