Human cytomegalovirus UL97 open reading frame encodes a protein that phosphorylates the antiviral nucleoside analogue ganciclovir.

Human cytomegalovirus (HCMV, a betaherpes virus) is the cause of serious disease in immunologically compromised individuals, including those with acquired immunodeficiency syndrome. One of the compounds used in the chemotherapy of HCMV infections is the nucleoside analogue 9-(1,3-dihydroxy-2-propoxymethyl)-guanine (ganciclovir). The mechanism of action of this drug is dependent on the formation of the nucleoside triphosphate, which is a strong inhibitor of the viral DNA polymerase. Thymidine kinase, which is encoded by many of the herpesviruses, catalyses the initial phosphorylation of ganciclovir. But there is no evidence for the coding of this enzyme by HCMV, and DNA sequence analysis of the HCMV genome has shown that there is no open reading frame characteristic of a herpesvirus thymidine kinase. Here we present biochemical and immunological evidence that the HCMV UL97 open reading frame codes for a protein capable of phosphorylating ganciclovir. This protein seems to be responsible for the selectivity of ganciclovir and will be useful tool in the understanding and refinement of the antiviral activity of new selective anti-HCMV compounds.     

锡原子多重线5p6s—5p~2分析

本文分析ＳｎＩ共振谱线组，线性拟合计算基态和第一激发态的谱项参数．增加校正项─ａＬ（Ｌ＋１）和ａＪ（Ｊ＋１），大大改善拟会精度     

微机字处理软件CAI开发方法的研究

本文阐述了研究计算机软件ＣＡＩ开发方法学的意义，并介绍了字处理软件Ｗｏｒｄｓｔａｒ的教学软件的开发方法．     

Φ12LMQ型钢筋冷拉机原理及主要参数选择

介绍了一种新型钢筋冷拉机的工作原理和主要参数：变形速度Ｖ△Ｌ、单位时间伸长率ε、送进速度Ｖ２、冷拉速度Ｖ１及其之间的关系．这种冷拉机具有生产率高、冷拉质量稳定等特点．     

铝电解过程控制信号的滤波与噪声解析模型的研究

将快速傅里叶变换技术和数字滤波器设计技术应用于铝电解过程控制信号的滤波与噪声解析模型中,在160kA中间点式下料预焙槽上的试验结果表明了模型的有效性。     

Estimation and testing of time-varying coefficient regression models in the presence of linear restrictions

A linear regression model with random walk coefficients is extended to allow for linear restrictions between the coefficients to be satisfied at each point in time. Estimation in this model is shown to be no more involved than estimation in the standard model. It is also demonstrated how, after a slight modification to the testing problem, classical test procedures may be applied to the problem of testing for such restrictions. The performance of the Lagrange Multiplier test for a variety of different restrictions is then investigated via simulation. An empirical application involving testing for homogeneity in a random walk coefficient version of the AIDS model is given.     

Nuclear localization and signalling activity of phosphoinositidase C beta in Swiss 3T3 cells.

The hydrolysis of phosphatidylinositol 4,5-bisphosphate (PtdInsP2) is a widespread receptor-coupled signalling system at the plasma membrane of most eukaryotic cells. The existence of an entirely separate nuclear phosphoinositide signalling system is suggested from evidence that purified nuclei synthesize PtdInsP2 and phosphatidylinositol 4-phosphate (PtdInsP) in vitro and that a transient decrease in the mass of these lipids occurs when Swiss 3T3 cells are cultured in the presence of insulin-like growth factor-1 (IGF-1). These IGF-1-dependent changes in inositol lipids coincide with an increase in nuclear diacyglycerol and precede translocation to the nucleus and activation of protein kinase C (refs 5, 6). Circumstantial evidence that links these changes with mitosis comes from the isolation of a 3T3 clone that expresses the type-1 IGF receptor and binds IGF-1 peptide but does not respond mitogenically or show transient mass changes in nuclear inositol lipids. A key question is how IGF-1 initiates the rapid breakdown of PtdInsP and PtdInsP2 in the nucleus. Here we present evidence that nuclei of 3T3 cells contain the beta-isozyme of phosphoinositidase C, whereas the gamma-isozyme is confined to the cytoplasm and that IGF-1 treatment stimulates exclusively the activity of nuclear phosphoinositidase C.     

油田水CaCO_3结垢反应的计算与应用

以热力学计算为基础，广泛采用油田水的数据．计算了ＣａＣＯ３的预测条件，并根据油田生产实际，建立了使用防垢剂时的预测模型．根据理论模型编制成计算机程序，并与文献结果和油田观测结果进行了对比，提出了临界浓度和临界稳定常数的计算和应用方法．为防垢剂的选择提供了理论依据，大量计算表明，预测结果与文献和现场观测结果相符．     

关于P（△）的强端点的性质

本文首先引进了单位园△上算子值解析函数族：Ｐ（△）＝｛ｆ（Ｚ），ｆ（Ｚ）＝Ｉ＋Ｂ（Ｉ）Ｚ＋Ｂ（２）Ｚ￣２＋…在△内解析，且Ｒｅｆ（Ｚ）＞０，Ｂ（ｎ）为Ｈｉｌｂｅｒｔ空间Ｈ上的正规算子，ｎ＝１，２…｝的强端点的概念，然后指出Ｐ（△）中形如Ｉ＋Ｂ（ｎ）Ｚ￣ｎ＋Ｂ（ｎ＋１）Ｚ￣（ｎ＋１）＋…的元素成Ｘ＿１Ｐ（△）的一个强端点的必要条件为Ｂ（ｎ）不是自伴可逆算子。     

Seasonality in human sleep.

The timing of sleep and sleep EEG parameters in 10 healthy male subjects were investigated in four seasons under controlled conditions. The phase of nocturnal sleep was delayed about one and a half hours in winter as compared to that in summer. The duration of stage 4 sleep decreased and REM sleep increased significantly in winter compared with summer. The seasonality in the timing of sleep can be explained by photoperiodic time cues, but the changes in sleep EEG parameters are difficult to explain in terms of photoperiod.