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251.
Protection of endangered desert plant species is frequently complicated by a lack of information about seeds, the most stress-resistant and populous life stage. We studied the relative locations of seeds and plants of a rare endangered herbaceous perennial, the Las Vegas bearpoppy ( Arctomecon californica ) in seven 1-ha sites randomly located within a 100-ha study area that was slated for development in Las Vegas, Nevada. We also measured the physical and biological attributes of the environment supporting the seeds and plants, including soil hardness, rock cover, cryptogamic crust cover, and associated vegetation. Arctomecon californica seed density was 0.651 seeds · m –2 , and seeds were found throughout the top 4 cm of soil. Seed viability among sites ranged from 26% to 79%; however, no significant changes between depths were detected. The spatial distribution of the seed bank coincided with the current A. californica adult distribution only in sites with both a high plant density and high numbers of seeds. Arctomecon californica adults occupied open areas within the gypsum environment that supported significantly less vegetative cover than the surrounding area. We observed a positive association between A. californica adults and shadscale ( Atriplex confertifolia ) and a negative association with 7 other plant species among sites. Sites with an indurated abiotic crust hosted higher numbers of A. californica adults than sites with softer soils. However, rock cover, cryptogamic crust cover, and soil hardness were poor explanatory factors for characterizing A. californica presence. We suggest that land managers should incorporate seed bank census into monitoring programs due to the extreme year-to-year fluctuations in A. californica plant population densities. A menudo la protección de especies de plantas desérticas en peligro de extinción se ve complicada por la falta de información sobre las semillas, la etapa de vida más abundante y resistente al estrés. Estudiamos la ubicación relativa de semillas y plantas de “Las Vegas bearpoppy” ( Arctomecon californica ), una planta perenne en peligro de extinción, en 7 sitios de 1 ha. localizados al azar dentro de un área de estudio de 100 ha que estaba destinada a la urbanización en Las Vegas, Nevada (E.U.A.). También medimos los atributos físicos y biológicos del ambiente que sostiene las semillas y plantas, tales como dureza del suelo, rocosidad, cobertura de costra cryptógama y vegetación asociada. La densidad de semillas de A. californica fue 0.651 semillas · m –2 y se observó en los 4 cm superiores del suelo. La viabilidad de las semillas varió del 26% al 79% entre los sitios; sin embargo, no detectamos cambios significativos entre profundidades. La distribución espacial del banco de semillas coincidió con la distribución actual de adultos de A. californica, sólo en sitios que tuvieron una alta densidad de plantas y altos números de semillas. Los adultos de A. californica ocuparon áreas abiertas dentro del ambiente de yeso que sostenía significativamente menos cobertura vegetal que las áreas circunvecinas. Observamos una asociación positiva entre los adultos de A. californica y el chamizo ( Atriplex confertifolia ) y una asociación negativa con siete otras especies de plantas entre sitios. Los sitios con una costra abiótica endurecida albergaron más adultos de A. californica que los sitios con suelos más blandos. No obstante, la cubierta rocosa, la costra cryptógama y la dureza del suelo fueron inadecuadas como factores para caracterizar la presencia de A. californica. Sugerimos que los manejadores de tierras deben incorporar censos de bancos de semillas en los programas de monitoreo, debido a las fluctuaciones extremas de un año a otro en la densidad poblacional de A. californica. Normal 0 MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman";} 相似文献
252.
Li M Zhao H Zhang X Wood LD Anders RA Choti MA Pawlik TM Daniel HD Kannangai R Offerhaus GJ Velculescu VE Wang L Zhou S Vogelstein B Hruban RH Papadopoulos N Cai J Torbenson MS Kinzler KW 《Nature genetics》2011,43(9):828-829
Through exomic sequencing of ten hepatitis C virus (HCV)-associated hepatocellular carcinomas (HCC) and subsequent evaluation of additional affected individuals, we discovered novel inactivating mutations of ARID2 in four major subtypes of HCC (HCV-associated HCC, hepatitis B virus (HBV)-associated HCC, alcohol-associated HCC and HCC with no known etiology). Notably, 18.2% of individuals with HCV-associated HCC in the United States and Europe harbored ARID2 inactivation mutations, suggesting that ARID2 is a tumor suppressor gene that is relatively commonly mutated in this tumor subtype. 相似文献
253.
Putoux A Thomas S Coene KL Davis EE Alanay Y Ogur G Uz E Buzas D Gomes C Patrier S Bennett CL Elkhartoufi N Frison MH Rigonnot L Joyé N Pruvost S Utine GE Boduroglu K Nitschke P Fertitta L Thauvin-Robinet C Munnich A Cormier-Daire V Hennekam R Colin E Akarsu NA Bole-Feysot C Cagnard N Schmitt A Goudin N Lyonnet S Encha-Razavi F Siffroi JP Winey M Katsanis N Gonzales M Vekemans M Beales PL Attié-Bitach T 《Nature genetics》2011,43(6):601-606
254.
Roman Goetzke Antonio Sechi Laura De Laporte Sabine Neuss Wolfgang Wagner 《Cellular and molecular life sciences : CMLS》2018,75(18):3297-3312
Mechanical stimulation affects growth and differentiation of stem cells. This may be used to guide lineage-specific cell fate decisions and therefore opens fascinating opportunities for stem cell biology and regenerative medicine. Several studies demonstrated functional and molecular effects of mechanical stimulation but on first sight these results often appear to be inconsistent. Comparison of such studies is hampered by a multitude of relevant parameters that act in concert. There are notorious differences between species, cell types, and culture conditions. Furthermore, the utilized culture substrates have complex features, such as surface chemistry, elasticity, and topography. Cell culture substrates can vary from simple, flat materials to complex 3D scaffolds. Last but not least, mechanical forces can be applied with different frequency, amplitude, and strength. It is therefore a prerequisite to take all these parameters into consideration when ascribing their specific functional relevance—and to only modulate one parameter at the time if the relevance of this parameter is addressed. Such research questions can only be investigated by interdisciplinary cooperation. In this review, we focus particularly on mesenchymal stem cells and pluripotent stem cells to discuss relevant parameters that contribute to the kaleidoscope of mechanical stimulation of stem cells. 相似文献
255.
Barton A Thomson W Ke X Eyre S Hinks A Bowes J Plant D Gibbons LJ;Wellcome Trust Case Control Consortium;YEAR Consortium;BIRAC Consortium Wilson AG Bax DE Morgan AW Emery P Steer S Hocking L Reid DM Wordsworth P Harrison P Worthington J 《Nature genetics》2008,40(10):1156-1159
The WTCCC study identified 49 SNPs putatively associated with rheumatoid arthritis at P = 1 x 10(-4) - 1 x 10(-5) (tier 3). Here we show that three of these SNPs, mapping to chromosome 10p15 (rs4750316), 12q13 (rs1678542) and 22q13 (rs3218253), are also associated (trend P = 4 x 10(-5), P = 4 x 10(-4) and P = 4 x 10(-4), respectively) in a validation study of 4,106 individuals with rheumatoid arthritis and an expanded reference group of 11,238 subjects, confirming them as true susceptibility loci in individuals of European ancestry. 相似文献
256.
Rosaria Scozzari Laura Ulizzi A. Ferracin M. Lunadei 《Cellular and molecular life sciences : CMLS》1980,36(6):645-646
Summary Phosphoglucomutase electrophoretic patterns have been studied in 60 tail homogenates ofTriturus cristatus carnifex. Our results show that the same sample produces a different electrophoretic pattern with homogenate ageing; a new band of intermediate mobility appears, together with the one produced by the fresh preparation. The phenomenon can mimic a true genetic polymorphism when differently stored samples are analyzed. 相似文献
257.
Laura Riboni Hortensia D'Albora Emilia Carlevaro R. Dominguez 《Cellular and molecular life sciences : CMLS》1969,25(7):754-755
Résumé Des greffons d'ovaire de rat impubère placés dans la chambre antérieure de l'il du mâle castré, après traitement avec 0,5 mg de dipropionate d'stradiol ou avec deux doses de 1 mg de progestérone, ont montré une évidente ovulation. On interprète ce fait comme dû á l'action des hormones injectées dans le système nerveux central et qui favorisent le mécanisme de l'ovulation. 相似文献
258.
259.
The sensing mechanisms of chemotactic bacteria allow them to respond sensitively to stimuli. Escherichia coli, for example, respond to changes in chemoattractant concentration of less than 10% over a range spanning six orders of magnitude. Sensitivity over this range depends on a nonlinear relationship between ligand concentration and output response. At low ligand concentrations, substantial amplification of the chemotactic signal is required; however, the mechanism responsible for this amplification remains unclear. Here we demonstrate that inter-receptor communication within a lattice acts to amplify and integrate sensory information. Synthetic multivalent ligands that interact through the low-abundance, galactose-sensing receptor Trg stabilize large clusters of chemoreceptors and markedly enhance signal output from these enforced clusters. On treatment with multivalent ligands, the response to the attractant serine is amplified by at least 100-fold. This amplification requires a full complement of chemoreceptors; deletion of the aspartate (Tar) or dipeptide (Tap) receptors diminishes the amplification of the serine response. These results demonstrate that the entire array is involved in sensing. This mode of information exchange has general implications for the processing of signals by cellular receptors. 相似文献
260.
Laura Foá-Tomasi F. Costanzo Gabriella Campadelli-Fiume 《Cellular and molecular life sciences : CMLS》1976,32(1):45-46
Summary The inhibition of RNA synthesis by -amanitin on vitro cultured cells is very slow. The action of various analogues of the toxin was tested and some of them proved more effective. Moreover pretreatment of cell cultures with DEAE-dextran greatly enhanced the effect of -amanitin.Acknowledgments. We thank ProfessorTh. Wieland and Dr.H. Faulstich (Max-Planck-Institut, Heidelberg) for the generous gift of amanitins. This investigation was supported by grant No. 74.00637.65 from C.N.R. (Roma). 相似文献