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981.
介绍了IEEE802.1x协议的起源、体系结构、技术特点等,指出了需要认证的端口有受控和非受控两种状态,没有通过认证前,只有EAP报文可以通过端口到达认证服务器,认证通过后,其他数据报文才可以通过。 相似文献
982.
转美洲拟鲽抗冻蛋白基因(afp)番茄D4代植株可溶性蛋白分析 总被引:1,自引:0,他引:1
对转美洲拟鲽抗冻蛋白基因(afp)的第四代番茄及其对照经低温处理后,对幼苗叶片组织可溶性蛋白进行聚丙烯酰胺凝胶电泳分析和紫外吸收法测定含量。发现转基因各组经低温诱导后的可溶性蛋白均比对照明显增加,聚丙烯酰胺凝胶电泳图谱发生变化。其中A-Ⅲ区电泳谱带迁移率加快,C区和B区语带染色加深.说明导入的afp基因已经表达,并使番茄获得抗寒性。 相似文献
983.
黄勇 《河海大学常州分校学报》1997,(3)
本文研究把碳输入到纯铁和IC—1.4Cr钢(S135)的激光表面合金化处理.这种处理工艺包括试样表面用石墨粉预涂层;若用连续CO_2激光表面熔化处理时,在各个处理工艺间反复使用石墨涂料,而合金化处理在薄层的熔化区域和栓孔两个区域中进行.从亚共晶到过共晶范围碳合金化最高达6%C,在过共晶范围发现大裂纹和松孔. 相似文献
984.
理论上对CARS转换效率的公式作了进一步的推导.给出了气体CARS转换效率与压强的关系公式.绘出了转换效率与压强关系的模拟曲线.并与实验结果进行了比较 相似文献
985.
k consisting of k clusters, with k > 2. Bottom-up agglomerative approaches are also commonly used to construct partitions,
and we discuss these in terms of worst-case performance for metric data sets. Our main contribution derives from a new restricted
partition formulation that requires each cluster to be an interval of a given ordering of the objects being clustered. Dynamic programming can optimally split such an ordering into a partition Pk for a large class of objectives that includes min-diameter. We explore a variety of ordering heuristics and show that our
algorithm, when combined with an appropriate ordering heuristic, outperforms traditional algorithms on both random and non-random
data sets. 相似文献
986.
987.
988.
T. Matsuoka T. Nishizaki Y. Ikeuchi Y. Okada K. Sumino 《Cellular and molecular life sciences : CMLS》1997,53(3):233-236
Effects of serotonin (5-HT) on cerebral cortical neurons were examined by patch clamp techniques. 5-HT produced a variety
of responses such as outward (19/73 patches/neurons), slow inward (15/73 patches/neurons), fast inward (8/73 patches/neurons),
and mixed currents (initially fast inward deflection followed by an outward response: 2/73 patches/neurons), with a latency
of 12 sec, 15 sec, 0 sec, and 0 sec respectively, at a holding potential of −60 mV in whole-cell patches. The fast inward
currents were again evoked by a selective 5-HT3 receptor agonist, 1-(m-chlorophenyl)-biguanide hydrochloride (CPBG). In the
cell-attached patch clamp configuration, 5-HT inside the patch pipette elicited single channel currents with slope conductances
of 42 pS and 132 pS (4/42 patches/neurons). CPBG inside the patch pipette evoked inward single channel currents with a lower
slope conductance of 41 pS (3/23 patches/neurons). In contrast, application of 5-HT or a 5-HT2 receptor agonist, α-methyl-5-hydroxytryptamine-maleate, outside the patch pipette induced outward single channel currents with a major slope
conductance of 140 pS (8/30 patches/neurons) or 135 pS (6/20 patches/neurons), respectively. These results indicate that the
outward and fast inward currents may be mediated respectively by the 5-HT2 receptor, which is coupled to a G-protein, and by the 5-HT3 receptor, which contains the non-selective cation channel, and that the mixed type may be caused by both the 5-HT2 and 5-HT3 receptors.
Received 27 September 1996; received after revision 4 November 1996; accepted 7 November 1996 相似文献
989.
Regulation of cyclooxygenase enzymes by nitric oxide 总被引:12,自引:0,他引:12
D. Salvemini 《Cellular and molecular life sciences : CMLS》1997,53(7):576-582
990.
J. W. M. Lagerberg J. VanSteveninck T. M. A. R. Dubbelman 《Cellular and molecular life sciences : CMLS》1997,53(3):257-262
The fluorescent dye Merocyanine 540 (MC540) is often used as a probe to monitor the molecular packing of phospholipids in
the outer leaflet of biomembranes. In a previous study we showed that the increased staining of erythrocytes with a perturbed
membrane structure was mainly due to an increase in the fluorescence yield of cell-bound MC540, rather than to an increase
of the number of bound molecules. Erythrocytes and ghosts exposed to continuous fluxes of H2O2 exhibited pronounced lipid peroxidation. Further, red blood cells subjected to this form of oxidative stress also showed
increased staining with MC540. It appeared that this was caused by a strong increase in binding of MC540, together with a
slight red shift of the fluorescence emission maximum and a small increase in the fluorescence yield of bound MC540. The changed
MC540 binding characteristics were not observed when lipid peroxidation was suppressed by the presence of the antioxidant
BHT in the incubation medium. However, open ghosts exposed to H2O2 showed no increase of MC540 binding, excluding a direct involvement of lipid peroxidation. Measurement of fluorescence emission
spectra and gel filtration studies showed that MC540 can bind to H2O2-exposed hemoglobin. Experiments with erythrocytes lysed in hypotonic medium after exposure to H2O2 revealed that peroxidation of lipids with H2O2 induced a non-specific permeabilization of the plasma membrane to MC540, thereby allowing MC540 to bind to the oxidatively
denatured, more hydrophobic hemoglobin. These results indicate that conclusions about packing of phospholipids in the outer
leaflet of the membrane based on increased MC540-staining should be drawn with care.
Received 27 September 1996; received after revision 5 November 1996; accepted 27 November 1996 相似文献