Specific binding of antigenic peptides to cell-associated MHC class I molecules

T lymphocytes recognize antigen in the form of peptides that associate with specific alleles of class I or class II major histocompatibility (MHC) molecules. By contrast with the clear MHC allele-specific binding of peptides to purified class II molecules purified solubilized class I molecules either bind relatively poorly or show degenerate specificity. Using photo-affinity labelling, we demonstrate here the specific interaction of peptides with cell-associated MHC class I molecules and show that this involves metabolically active processes.     

Structure of the detoxification catalyst mercuric ion reductase from Bacillus sp. strain RC607

Several hundred million tons of toxic mercurials are dispersed in the biosphere. Microbes can detoxify organo-mercurials and mercury salts through sequential action of two enzymes, organomercury lyase and mercuric ion reductase (MerA). The latter, a homodimer with homology to the FAD-dependent disulphide oxidoreductases, catalyses the reaction NADPH + Hg(II)----NADP+ + H+ + Hg(0), one of the very rare enzymic reactions with metal substrates. Human glutathione reductase serves as a reference molecule for FAD-dependent disulphide reductases and between its primary structure and that of MerA from Tn501 (Pseudomonas), Tn21 (Shigella), p1258 (Staphylococcus) and Bacillus, 25-30% of the residues have been conserved. All MerAs have a C-terminal extension about 15 residues long but have very varied N termini. Although the enzyme from Streptomyces lividans has no addition, from Pseudomonas aeruginosa Tn501 and Bacillus sp. strain RC607 it has one and two copies respectively of a domain of 80-85 residues, highly homologous to MerP, the periplasmic component of proteins encoded by the mer operon. These domains can be proteolytically cleaved off without changing the catalytic efficiency. We report here the crystal structure of MerA from the Gram-positive bacterium Bacillus sp. strain RC607. Analysis of its complexes with nicotinamide dinucleotide substrates and the inhibitor Cd(II) reveals how limited structural changes enable an enzyme to accept as substrate what used to be a dangerous inhibitor. Knowledge of the mode of mercury ligation is a prerequisite for understanding this unique detoxification mechanism.     

Genetic and photoperiodic control of an avian reproductive cycle

European blackcaps,Sylvia atricapilla, with one breeding season per year, have a single-peaked annual testes cycle. However, African conspecifics from the Cape Verde Islands with two breeding seasons per annum demonstrate a two-peaked cycle. Both population-specific cycles reflect differences in the respective endogenous circannual rhythms. Experimental hybridization of birds of the two populations resulted in an intermediate pattern of testes cycle, thus demonstrating that there are genetic components for some temporal aspects in an avian reproductive cycle. Another characteristic of the African birds, their extremely rapid juvenile development and early sexual maturity (at an age of 5–6 months) proved largely to be a photoperiodic (short-day) effect in birds hatched in autumn. The same effect could also be induced in European conspecifics exposed to correspondingly short day-lengths.     

Characterization of the rough endoplasmic reticulum ribosome-binding activity.

The rough endoplasmic reticulum membranes of mammalian cells contain specific ribosome-binding sites. A purification to apparent homogeneity of a negatively charged protein (ERp180) of relative molecular mass 180,000 (180 K) was reported which was proposed to function as a rough endoplasmic reticulum ribosome receptor. We report here that ribosome-binding site activity quantitatively solubilized from rough endoplasmic reticulum membranes does not cofractionate with ERp180. By contrast, ribosome-binding site activity fractionates as a much smaller, positively charged protein.     

Genotoxic effects of dithane M-45 on the bone marrow cells of mice in vivo

Genotoxic effects of dithane M-45 were studied on the bone marrow cells of male albino mice (Lacca strain) in vivo. Different doses (30 mg, 40 mg and 300 mg/kg b.wt) of dithane M-45 were injected intraperitoneally and their effects were investigated after time intervals of 1, 2, 5 and 10 days. The chromosomal aberrations observed in the bone marrow cells of male mice after treatment with dithane M-45 were fragments, rings, dicentric chromosomes, terminal chromatid deletions, chromatid gaps and breaks. In addition to these chromosomal aberrations, physiological effects such as uneven stretching of chromatin material, end-to-end chromosomal associations, exchange configurations, clumping, stickiness and centromeric associations were also observed.     

Cartesian causation: body–body interaction, motion, and eternal truths

There is considerable debate among scholars over whether Descartes allowed for genuine body–body interaction. I begin by considering Michael Della Rocca’s recent claim that Descartes accepted such interaction, and that his doctrine of the creation of the eternal truths indicates how this interaction could be acceptable to him. Though I agree that Descartes was inclined to accept real bodily causes of motion, I differ from Della Rocca in emphasizing that his ontology ultimately does not allow for them. This is not the end of the story however, since two of Descartes’s successors offered incompatible ways of developing his conflicted account of motion. I contrast the occasionalist view of Nicolas Malebranche that changes in motion derive directly from divine volitions with the non-occasionalist claim of Pierre-Sylvain Regis that such changes derive from a nature distinct from God. In light of Della Rocca’s interpretation, it is noteworthy that the issue of eternal truths is relevant to both alternative accounts. Indeed, Regis took the doctrine that such truths are created to provide crucial support for his alternative to an occasionalist account of body–body interaction. What does not help Della Rocca, however, is that Regis’s view of motion requires a fundamental revision of Descartes’s ontology.     

秋水仙素诱导青虾次级卵母细胞二倍体初探

以青虾卵巢为材料，采用秋水仙素诱导多倍体次级卵母细胞．经滴片试验，确立此实验最佳滴片高度为1．5cm，通过设置5个秋水仙素浓度(0．08～0．40mg／mL)及3个作用时间(6～24h)，观察发生染色体加倍的细胞相对数目．实验结果表明：在使用秋水仙素的浓度为0．08～0．40mg／mL，处理6～24h时都可以获得多倍体卵细胞．在秋水仙素浓度是0．32mg／mL，处理6h可得到最高二倍体突变率(32．58％)．还测试了0．32mg／mL COM作用6h时ATPase活性与生理盐水对照组的变化，结果显示COM会在一定程度上影响细胞的新陈代谢．但是50％左右的成活率表明，这种诱导突变的方法仍具有一定的应用价值．     

基于Windows NT的HRPS-Ⅱ设备的控制系统设计

介绍了华中科技大学开发的HRPS-Ⅱ型选择性激光烧结设备的体系结构，根据选择性激光烧结的工作原理并结合该系统的硬件结构开发了基于NT操作系统下的控制软件．该控制软件是一种分层的结构，各个不同优先级的任务在不同的层面上运行，实时性强的部分运行在硬件中断级别上，对控制软件的关键性技术进行了描述．通过试验运行，该设备成功地加工出了复杂的原型件，证明控制系统开发是成功的．     

Darstellung doppelbrechender Lipoidtropfen aus dem Serum

Summary (1) Doubly refracting lipoid droplets can be demonstrated in normal and pathological sera by means of alcohol ether extraction. This is not possible with ether extraction alone.(2) These lipoid droplets can be demonstrated only, or almost only, when they are precipitated out of alcohol or ether alcohol     

Hydrobiologische Anstalt Plön der Max-Planck-Gesellschaft zur Förderung der Wissenschaften

Ohne Zusammenfassung