全文获取类型
收费全文 | 23292篇 |
免费 | 703篇 |
国内免费 | 227篇 |
专业分类
系统科学 | 1439篇 |
丛书文集 | 382篇 |
教育与普及 | 72篇 |
理论与方法论 | 329篇 |
现状及发展 | 7052篇 |
研究方法 | 776篇 |
综合类 | 13651篇 |
自然研究 | 521篇 |
出版年
2018年 | 781篇 |
2017年 | 787篇 |
2016年 | 507篇 |
2013年 | 280篇 |
2012年 | 727篇 |
2011年 | 1935篇 |
2010年 | 1156篇 |
2009年 | 788篇 |
2008年 | 1026篇 |
2007年 | 1355篇 |
2006年 | 609篇 |
2005年 | 582篇 |
2004年 | 514篇 |
2003年 | 454篇 |
2002年 | 372篇 |
2001年 | 507篇 |
2000年 | 560篇 |
1999年 | 411篇 |
1992年 | 288篇 |
1991年 | 255篇 |
1990年 | 250篇 |
1989年 | 229篇 |
1988年 | 209篇 |
1987年 | 226篇 |
1986年 | 203篇 |
1985年 | 245篇 |
1984年 | 235篇 |
1983年 | 159篇 |
1982年 | 172篇 |
1981年 | 157篇 |
1980年 | 199篇 |
1979年 | 445篇 |
1978年 | 340篇 |
1977年 | 367篇 |
1976年 | 264篇 |
1975年 | 322篇 |
1974年 | 446篇 |
1973年 | 379篇 |
1972年 | 360篇 |
1971年 | 421篇 |
1970年 | 484篇 |
1969年 | 425篇 |
1968年 | 407篇 |
1967年 | 443篇 |
1966年 | 384篇 |
1965年 | 301篇 |
1959年 | 155篇 |
1958年 | 231篇 |
1957年 | 179篇 |
1955年 | 154篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
71.
72.
Transgenesis in fish 总被引:1,自引:0,他引:1
Gene transfer into fish embryo is being performed in several species (trout, salmon, carps, tilapia, medaka, goldfish, zebrafish, loach, catfish, etc.). In most cases, pronuclei are not visible and microinjection must be done into the cytoplasm of early embryos. Several million copies of the gene are generally injected. In medaka, transgenesis was attempted by injection of the foreign gene into the nucleus of oocyte. Several reports indicate that the injected DNA was rapidly replicated in the early phase of embryo development, regardless of the origin and the sequence of the foreign DNA. The survival of the injected embryos was reasonably good and a large number reached maturity. The proportion of transgenic animals ranged from 1 to 50% or more, according to species and to experimentators. The reasons for this discrepancy have not been elucidated. In all species, the transgenic animals were mosaic. The copy number of the foreign DNA was different in the various tissues of an animal and a proportion lower than 50% of F1 offsprings received the gene from their parents. This suggests that the foreign DNA was integrated into the fish genome at the two cells stage or later. An examination of the integrated DNA in different cell types of an animal revealed that integration occurred mainly during early development. The transgene was found essentially unrearranged in the fish genome of the founders and offsprings. The transgenes were therefore stably transmitted to progeny in a Mendelian fashion. Southern blot analysis revealed the presence of possible junction fragments and also of minor bands which may result from a rearrangement of the injected DNA. In all species, the integrated DNA appeared mainly as random end-to-end concatemers. In adult trout blood cells, a small proportion of the foreign DNA was maintained in the form of non-integrated concatemers, as judged by the existence of end fragments. The transgenes were generally only poorly expressed. The majority of the injected gene constructs contained essentially mammalian or higher vertebrates sequences. The comparison of the expression efficiency of these constructs in transfected fish and mammalian cells indicates that some of the mammalian DNA sequences are most efficiently understood by the fish cell machinery. Chloramphenicol acetyl transferase gene under the control of promoters from Rous sarcoma virus, and human cytomegalovirus, was expressed in several tissues of transgenic fish. Chicken delta-crystallin gene was expressed in several tissues of transgenic fish.(ABSTRACT TRUNCATED AT 400 WORDS) 相似文献
73.
钢琴副科课是我国音乐院校针对非钢琴专业的学生开设的一门钢琴课程.本文以如何上好钢琴副科课为探讨的主要目的,从基础和提高视谱能力这两个角度来展开讨论,力图使学生学习更富有热情和效率,对知识的掌握更为主动和全面. 相似文献
74.
研究以水泥处理固体有机物时,有机物对水泥水化的影响。以膜流模式说明水份在水泥浆体内传输的现象。水膜存在半干之水泥浆体,其厚度小于100nm,膜流由离分压所驱动,而离分压来自分子间作用力。膜拟结果发现,水泥浆体内局部位置可用以进行水化之水量为Q=-Aslv/6vπ。当有机物添加在水泥浆体内时,会影响Hamaker常数,Aslv因此可能使膜流不稳定或完全抑制膜流,因而使水泥浆体内局部缺水,影响水化进行,造成成品强度降低,影响品质。 相似文献
75.
Frank Waaldijk 《Foundations of Science》2005,10(3):249-324
We discuss the foundations of constructive mathematics, including recursive mathematics and intuitionism, in relation to classical
mathematics. There are connections with the foundations of physics, due to the way in which the different branches of mathematics
reflect reality. Many different axioms and their interrelationship are discussed. We show that there is a fundamental problem
in BISH (Bishop’s school of constructive mathematics) with regard to its current definition of ‘continuous function’. This problem
is closely related to the definition in BISH of ‘locally compact’. Possible approaches to this problem are discussed. Topology seems to be a key to understanding many
issues. We offer several new simplifying axioms, which can form bridges between the various branches of constructive mathematics
and classical mathematics (‘reuniting the antipodes’). We give a simplification of basic intuitionistic theory, especially
with regard to so-called ‘bar induction’. We then plead for a limited number of axiomatic systems, which differentiate between
the various branches of mathematics. Finally, in the appendix we offer BISH an elegant topological definition of ‘locally compact’, which unlike the current definition is equivalent to the usual classical
and/or intuitionistic definition in classical and intuitionistic mathematics, respectively. 相似文献
76.
林国顺 《哈尔滨商业大学学报(自然科学版)》1996,(3)
以计算机模拟为辅助工具 ,讨论了机器人路径规划的算法 ,提出一种简化算法二次规划 ,既可避免组合爆炸 ,又能得到一个较优的解 ,即满意解 相似文献
77.
Induction of hemopoietic chimerism in the caprine fetus by intraperitoneal injection of fetal liver cells 总被引:1,自引:0,他引:1
R. D. Pearce D. Kiehm D. T. Armstrong P. B. Little J. W. Callahan L. R. Klunder J. T. R. Clarke 《Cellular and molecular life sciences : CMLS》1989,45(3):307-308
Summary Intraperitoneal injection of allogeneic liver cells from 43-day-old male fetuses into normal 60-day female goat fetuses resulted in persistent hemopoietic chimerism in surviving recipients without clinical evidence of graft-versus-host disease. Transplantation of normal fetal liver cells into preimmunocompetent goat fetuses affected with -D-mannosidosis may provide an alternative strategy for evaluating hemopoietic stem cell transplantation in the treatment of human lysosomal storage diseases. 相似文献
78.
Summary Monarch butterflies sequester cardenolides from their larval host plants in the milkweed genusAsclepias for use in defense against predation. Of 108Asclepias species in North America, monarchs are known to feed as larvae on 27. Research on 11 of these has shown that monarchs sequester cardenolides most effectively, to an asymptote of approximately 350 g/0.1 g dry butterfly, from plants with intermediate cardenolide contents rather than from those with very high or very low cardenolide contents. SinceAsclepias host plant species are distributed widely in space and time across the continent, monarchs exploit them by migration between breeding and overwintering areas. After overwintering in central Mexico, spring migrants east of the Rocky Mountains exploit three predominantAsclepias species in the southern USA that have moderately high cardenolide contents. Monarchs sequester cardenolides very effectively from these species. First generation butterflies are thus well protected against predators and continue the migration north. Across the northern USA and southern Canada most summer breeding occurs on a fourthAsclepias species and in autumn most of these monarchs migrate back to Mexican overwintering sites. The ecological implications of this cycle of cardenolide sequestration for the evolution of monarch migration are discussed. 相似文献
79.
B. Lindblad W. E. Burkel T. W. Wakefield L. M. Graham J. C. Stanley 《Cellular and molecular life sciences : CMLS》1988,44(3):223-224
Summary The most important effect of dihydroergotamine is venoconstriction, but certain metabolic effects and changes in vessel prostanoid activity have also been suggested. In this study endothelial cell production of 6-keto PGF1 and TxB2 was quantitated in vitro. No evidence of altered prostanoid production was noted after incubation with dihydroergotamine (exposure ranging from 5×10–3 to 5×10–7 g/l). Similarly, no effect of dihydroergotamine on the growth rates of endothelial cells or smooth muscle cells in vitro was documented. 相似文献
80.
A. Alkofahi J. K. Rupprecht D. L. Smith Ch. -J. Chang J. L. McLaughlin 《Cellular and molecular life sciences : CMLS》1988,44(1):83-85
Summary Using brine shrimp lethality for activity-directed fractionation, goniothalamicin (I), a new tetrahydroxy-mono-tetrahydrofuran fatty acid -lactone (acetogenin), has been isolated from ethanolic extracts of the stem bark ofGoniothalamus giganteus Hook. f., Thomas (Annonaceae). This novel compound was found to be cytotoxic and insecticidal and inhibited the formation of crown gall tumors on potato discs. Annonacin (II), the only other reported mono-tetrahydrofuran acetogenin, was also isolated; the previously reported 9ASK (astrocytoma reversal) activity ofII was confirmed, andII is now also found to be weakly active against 3PS murine leukemia. 相似文献