Rheogoniometric viscosity measurements of whole human blood at minimal shear rates down to 0.0009 sec−1

Zusammenfassung Messungen menschlichen Gesamtblutvolumens bei sehr niedrigen Schubzeiten bis 0,0009 sec^–1 (modifizierter Weissenberg-Rheogoniometer). Es wird gefunden, dass das Blut kein Newton'sches Verhalten und bei diesen niedrigen Schubzeitableitungen eine Fliessspannung aufweist.     

Genome sequence of Silicibacter pomeroyi reveals adaptations to the marine environment

Since the recognition of prokaryotes as essential components of the oceanic food web, bacterioplankton have been acknowledged as catalysts of most major biogeochemical processes in the sea. Studying heterotrophic bacterioplankton has been challenging, however, as most major clades have never been cultured or have only been grown to low densities in sea water. Here we describe the genome sequence of Silicibacter pomeroyi, a member of the marine Roseobacter clade (Fig. 1), the relatives of which comprise approximately 10-20% of coastal and oceanic mixed-layer bacterioplankton. This first genome sequence from any major heterotrophic clade consists of a chromosome (4,109,442 base pairs) and megaplasmid (491,611 base pairs). Genome analysis indicates that this organism relies upon a lithoheterotrophic strategy that uses inorganic compounds (carbon monoxide and sulphide) to supplement heterotrophy. Silicibacter pomeroyi also has genes advantageous for associations with plankton and suspended particles, including genes for uptake of algal-derived compounds, use of metabolites from reducing microzones, rapid growth and cell-density-dependent regulation. This bacterium has a physiology distinct from that of marine oligotrophs, adding a new strategy to the recognized repertoire for coping with a nutrient-poor ocean.     

Decoherence of quantum superpositions through coupling to engineered reservoirs

The theory of quantum mechanics applies to closed systems. In such ideal situations, a single atom can, for example, exist simultaneously in a superposition of two different spatial locations. In contrast, real systems always interact with their environment, with the consequence that macroscopic quantum superpositions (as illustrated by the 'Schrodinger's cat' thought-experiment) are not observed. Moreover, macroscopic superpositions decay so quickly that even the dynamics of decoherence cannot be observed. However, mesoscopic systems offer the possibility of observing the decoherence of such quantum superpositions. Here we present measurements of the decoherence of superposed motional states of a single trapped atom. Decoherence is induced by coupling the atom to engineered reservoirs, in which the coupling and state of the environment are controllable. We perform three experiments, finding that the decoherence rate scales with the square of a quantity describing the amplitude of the superposition state.     

hCds1-mediated phosphorylation of BRCA1 regulates the DNA damage response

Mutations in the BRCA1 (ref. 1) tumour suppressor gene are found in almost all of the families with inherited breast and ovarian cancers and about half of the families with only breast cancer. Although the biochemical function of BRCA1 is not well understood, it is important for DNA damage repair and cell-cycle checkpoint. BRCA1 exists in nuclear foci but is hyperphosphorylated and disperses after DNA damage. It is not known whether BRCA1 phosphorylation and dispersion and its function in DNA damage response are related. In yeast the DNA damage response and the replication-block checkpoint are mediated partly through the Cds1 kinase family. Here we report that the human Cds1 kinase (hCds1/Chk2) regulates BRCA1 function after DNA damage by phosphorylating serine 988 of BRCA1. We show that hCds1 and BRCA1 interact and co-localize within discrete nuclear foci but separate after gamma irradiation. Phosphorylation of BRCA1 at serine 988 is required for the release of BRCA1 from hCds1. This phosphorylation is also important for the ability of BRCA1 to restore survival after DNA damage in the BRCA1-mutated cell line HCC1937.     

Localization of type 1 diabetes susceptibility to the MHC class I genes HLA-B and HLA-A

The major histocompatibility complex (MHC) on chromosome 6 is associated with susceptibility to more common diseases than any other region of the human genome, including almost all disorders classified as autoimmune. In type 1 diabetes the major genetic susceptibility determinants have been mapped to the MHC class II genes HLA-DQB1 and HLA-DRB1 (refs 1-3), but these genes cannot completely explain the association between type 1 diabetes and the MHC region. Owing to the region's extreme gene density, the multiplicity of disease-associated alleles, strong associations between alleles, limited genotyping capability, and inadequate statistical approaches and sample sizes, which, and how many, loci within the MHC determine susceptibility remains unclear. Here, in several large type 1 diabetes data sets, we analyse a combined total of 1,729 polymorphisms, and apply statistical methods-recursive partitioning and regression-to pinpoint disease susceptibility to the MHC class I genes HLA-B and HLA-A (risk ratios >1.5; P(combined) = 2.01 x 10(-19) and 2.35 x 10(-13), respectively) in addition to the established associations of the MHC class II genes. Other loci with smaller and/or rarer effects might also be involved, but to find these, future searches must take into account both the HLA class II and class I genes and use even larger samples. Taken together with previous studies, we conclude that MHC-class-I-mediated events, principally involving HLA-B*39, contribute to the aetiology of type 1 diabetes.     

Warming trends in Asia amplified by brown cloud solar absorption

Atmospheric brown clouds are mostly the result of biomass burning and fossil fuel consumption. They consist of a mixture of light-absorbing and light-scattering aerosols and therefore contribute to atmospheric solar heating and surface cooling. The sum of the two climate forcing terms-the net aerosol forcing effect-is thought to be negative and may have masked as much as half of the global warming attributed to the recent rapid rise in greenhouse gases. There is, however, at least a fourfold uncertainty in the aerosol forcing effect. Atmospheric solar heating is a significant source of the uncertainty, because current estimates are largely derived from model studies. Here we use three lightweight unmanned aerial vehicles that were vertically stacked between 0.5 and 3 km over the polluted Indian Ocean. These unmanned aerial vehicles deployed miniaturized instruments measuring aerosol concentrations, soot amount and solar fluxes. During 18 flight missions the three unmanned aerial vehicles were flown with a horizontal separation of tens of metres or less and a temporal separation of less than ten seconds, which made it possible to measure the atmospheric solar heating rates directly. We found that atmospheric brown clouds enhanced lower atmospheric solar heating by about 50 per cent. Our general circulation model simulations, which take into account the recently observed widespread occurrence of vertically extended atmospheric brown clouds over the Indian Ocean and Asia, suggest that atmospheric brown clouds contribute as much as the recent increase in anthropogenic greenhouse gases to regional lower atmospheric warming trends. We propose that the combined warming trend of 0.25 K per decade may be sufficient to account for the observed retreat of the Himalayan glaciers.     

一个基于启发式分离向量搜索的凸多面体碰撞检测算法

碰撞检测是自然环境计算机模拟的基础,机器人等领域有着广泛的应用,本文提出了一个用于多面体碰撞检测的快速算法HS-jump,HP-jump算法建立了一个有效的检测模型,当两个凸多面体碰撞时,可以快速的报告它们之间碰撞, 当两个物体分离时,该算法提供了一个启发式搜索策略,用于快速的搜索分离物体的分离向量两个分离物体的分离平面的法 向量,与已有的算法相比,HS-jump算法可以更平衡的处理碰撞、分离的情况。